Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig
Spermatogenesis is an advanced biological process, relying on intricate interactions between somatic and germ cells in testes. Investigating various cell types is challenging because of cellular heterogeneity. Single-cell RNA sequencing (scRNA-seq) offers a method to analyze cellular heterogeneity....
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MDPI AG
2024-12-01
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| Series: | Current Issues in Molecular Biology |
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| author | Zunqiang Yan Qiaoli Yang Pengfei Wang Shuangbao Gun |
| author_facet | Zunqiang Yan Qiaoli Yang Pengfei Wang Shuangbao Gun |
| author_sort | Zunqiang Yan |
| collection | DOAJ |
| description | Spermatogenesis is an advanced biological process, relying on intricate interactions between somatic and germ cells in testes. Investigating various cell types is challenging because of cellular heterogeneity. Single-cell RNA sequencing (scRNA-seq) offers a method to analyze cellular heterogeneity. In this research, we performed 10× Genomics scRNA-seq to conduct an unbiased single-cell transcriptomic analysis in Hezuo pig (HZP) testis at one month of age during prepuberty. We collected 14,276 cells and identified 8 cell types (including 2 germ cells types and 6 somatic cell types). Pseudo-timing analysis demonstrated that Leydig cells (LCs) and myoid cells (MCs) originated from a shared progenitor cell lineage. Moreover, the functional enrichment analyses showed that the genes of differential expression were enriched in spermatogonia (SPG) and were enriched in the cell cycle, reproduction, and spermatogenesis. Expressed genes in spermatocytes (SPCs) were enriched in the cAMP, cell cycle, male gamete generation, reproductive system development, and sexual reproduction, while growth hormone synthesis, gamete generation, reproductive process, and spermine synthase activity were enriched in Sertoli cells (SCs). Additionally, chemokine, B cell receptor, activation of immune response, and enzyme binding were enriched in macrophages. Our study investigated transcriptional alterations across different cell types during spermatogenesis, yielding new understandings of spermatogenic processes and cell development. This research delivers an exploration of spermatogenesis and testicular cell biology in HZP, establishing the groundwork for upcoming breeding initiatives. |
| format | Article |
| id | doaj-art-337a9e1073eb46ad8a4b6d06759bb755 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-337a9e1073eb46ad8a4b6d06759bb7552025-01-24T13:27:23ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-014711010.3390/cimb47010010Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo PigZunqiang Yan0Qiaoli Yang1Pengfei Wang2Shuangbao Gun3College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaSpermatogenesis is an advanced biological process, relying on intricate interactions between somatic and germ cells in testes. Investigating various cell types is challenging because of cellular heterogeneity. Single-cell RNA sequencing (scRNA-seq) offers a method to analyze cellular heterogeneity. In this research, we performed 10× Genomics scRNA-seq to conduct an unbiased single-cell transcriptomic analysis in Hezuo pig (HZP) testis at one month of age during prepuberty. We collected 14,276 cells and identified 8 cell types (including 2 germ cells types and 6 somatic cell types). Pseudo-timing analysis demonstrated that Leydig cells (LCs) and myoid cells (MCs) originated from a shared progenitor cell lineage. Moreover, the functional enrichment analyses showed that the genes of differential expression were enriched in spermatogonia (SPG) and were enriched in the cell cycle, reproduction, and spermatogenesis. Expressed genes in spermatocytes (SPCs) were enriched in the cAMP, cell cycle, male gamete generation, reproductive system development, and sexual reproduction, while growth hormone synthesis, gamete generation, reproductive process, and spermine synthase activity were enriched in Sertoli cells (SCs). Additionally, chemokine, B cell receptor, activation of immune response, and enzyme binding were enriched in macrophages. Our study investigated transcriptional alterations across different cell types during spermatogenesis, yielding new understandings of spermatogenic processes and cell development. This research delivers an exploration of spermatogenesis and testicular cell biology in HZP, establishing the groundwork for upcoming breeding initiatives.https://www.mdpi.com/1467-3045/47/1/10scRNA-seqHezuo pigspermatogenesistestis |
| spellingShingle | Zunqiang Yan Qiaoli Yang Pengfei Wang Shuangbao Gun Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig Current Issues in Molecular Biology scRNA-seq Hezuo pig spermatogenesis testis |
| title | Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig |
| title_full | Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig |
| title_fullStr | Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig |
| title_full_unstemmed | Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig |
| title_short | Transcriptional Profiling of Testis Development in Pre-Sexually-Mature Hezuo Pig |
| title_sort | transcriptional profiling of testis development in pre sexually mature hezuo pig |
| topic | scRNA-seq Hezuo pig spermatogenesis testis |
| url | https://www.mdpi.com/1467-3045/47/1/10 |
| work_keys_str_mv | AT zunqiangyan transcriptionalprofilingoftestisdevelopmentinpresexuallymaturehezuopig AT qiaoliyang transcriptionalprofilingoftestisdevelopmentinpresexuallymaturehezuopig AT pengfeiwang transcriptionalprofilingoftestisdevelopmentinpresexuallymaturehezuopig AT shuangbaogun transcriptionalprofilingoftestisdevelopmentinpresexuallymaturehezuopig |