<i>In Vitro</i> and <i>In Vivo</i> Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate <i>Lactobacillus reuteri</i> FW2
Microorganisms synthesize diverse types of exopolysaccharides (EPSs). EPSs with varying structural and physical properties can demonstrate unique health benefits, which allow for their tailored applications as functional foods such as prebiotics. Levan, a fructose-based EPS, is gaining considerable...
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2025-01-01
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| author | Waqar Ahmad Anam Nasir Satya Prakash Azam Hayat Mujaddad ur Rehman Shazia Khaliq Kalsoom Akhtar Munir Ahmad Anwar Nayla Munawar |
| author_facet | Waqar Ahmad Anam Nasir Satya Prakash Azam Hayat Mujaddad ur Rehman Shazia Khaliq Kalsoom Akhtar Munir Ahmad Anwar Nayla Munawar |
| author_sort | Waqar Ahmad |
| collection | DOAJ |
| description | Microorganisms synthesize diverse types of exopolysaccharides (EPSs). EPSs with varying structural and physical properties can demonstrate unique health benefits, which allow for their tailored applications as functional foods such as prebiotics. Levan, a fructose-based EPS, is gaining considerable attention as an effective prebiotic to support the growth of beneficial gut bacteria. Consequently, this enhances digestive health, boosts the immune system, and reduces the risk of chronic diseases. Unfortunately, limited studies are available on levan-type EPSs to demonstrate their role as prebiotics. Therefore, in this study, we conducted in vitro and in vivo experiments, concerning intestinal cell integrity and metabolic syndrome, to assess the therapeutic potential of levan derived from <i>Lactobacillus reuteri</i> FW2. The in vitro experimental results revealed that levan improved the survival of impaired HT-29 epithelial cells of the intestine and also exerted antioxidant effects. In the in vivo experiments, mice fed with levan-supplemented feed exhibited low body weight gain, blood glucose, and serum cholesterol levels compared to the control group. These findings highlight the biotherapeutic potential of <i>L. reuteri</i> FW2-derived levan for improving metabolic syndrome and its associated aspects. It also signifies the need for a further detailed investigation based on clinical trials to include levan in dietary supplements for improved health and well-being. |
| format | Article |
| id | doaj-art-336b81ff43a645aa914db8d58438d56b |
| institution | Kabale University |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-336b81ff43a645aa914db8d58438d56b2025-01-24T13:38:44ZengMDPI AGLife2075-17292025-01-011518910.3390/life15010089<i>In Vitro</i> and <i>In Vivo</i> Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate <i>Lactobacillus reuteri</i> FW2Waqar Ahmad0Anam Nasir1Satya Prakash2Azam Hayat3Mujaddad ur Rehman4Shazia Khaliq5Kalsoom Akhtar6Munir Ahmad Anwar7Nayla Munawar8Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, PakistanIndustrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, PakistanBiomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC H3A 2B4, CanadaDepartment of Microbiology, Abbottabad University of Science and Technology, Havelian, Abbottabad 22500, PakistanDepartment of Microbiology, Abbottabad University of Science and Technology, Havelian, Abbottabad 22500, PakistanIndustrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, PakistanIndustrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, PakistanIndustrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Faisalabad 38000, PakistanDepartment of Chemistry, College of Sciences, United Arab Emirates University, Al-Ain 15551, United Arab EmiratesMicroorganisms synthesize diverse types of exopolysaccharides (EPSs). EPSs with varying structural and physical properties can demonstrate unique health benefits, which allow for their tailored applications as functional foods such as prebiotics. Levan, a fructose-based EPS, is gaining considerable attention as an effective prebiotic to support the growth of beneficial gut bacteria. Consequently, this enhances digestive health, boosts the immune system, and reduces the risk of chronic diseases. Unfortunately, limited studies are available on levan-type EPSs to demonstrate their role as prebiotics. Therefore, in this study, we conducted in vitro and in vivo experiments, concerning intestinal cell integrity and metabolic syndrome, to assess the therapeutic potential of levan derived from <i>Lactobacillus reuteri</i> FW2. The in vitro experimental results revealed that levan improved the survival of impaired HT-29 epithelial cells of the intestine and also exerted antioxidant effects. In the in vivo experiments, mice fed with levan-supplemented feed exhibited low body weight gain, blood glucose, and serum cholesterol levels compared to the control group. These findings highlight the biotherapeutic potential of <i>L. reuteri</i> FW2-derived levan for improving metabolic syndrome and its associated aspects. It also signifies the need for a further detailed investigation based on clinical trials to include levan in dietary supplements for improved health and well-being.https://www.mdpi.com/2075-1729/15/1/89levanprebioticsintestinal healthantioxidant<i>Lactobacillus</i> |
| spellingShingle | Waqar Ahmad Anam Nasir Satya Prakash Azam Hayat Mujaddad ur Rehman Shazia Khaliq Kalsoom Akhtar Munir Ahmad Anwar Nayla Munawar <i>In Vitro</i> and <i>In Vivo</i> Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate <i>Lactobacillus reuteri</i> FW2 Life levan prebiotics intestinal health antioxidant <i>Lactobacillus</i> |
| title | <i>In Vitro</i> and <i>In Vivo</i> Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate <i>Lactobacillus reuteri</i> FW2 |
| title_full | <i>In Vitro</i> and <i>In Vivo</i> Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate <i>Lactobacillus reuteri</i> FW2 |
| title_fullStr | <i>In Vitro</i> and <i>In Vivo</i> Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate <i>Lactobacillus reuteri</i> FW2 |
| title_full_unstemmed | <i>In Vitro</i> and <i>In Vivo</i> Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate <i>Lactobacillus reuteri</i> FW2 |
| title_short | <i>In Vitro</i> and <i>In Vivo</i> Interventions Reveal the Health Benefits of Levan-Type Exopolysaccharide Produced by a Fish Gut Isolate <i>Lactobacillus reuteri</i> FW2 |
| title_sort | i in vitro i and i in vivo i interventions reveal the health benefits of levan type exopolysaccharide produced by a fish gut isolate i lactobacillus reuteri i fw2 |
| topic | levan prebiotics intestinal health antioxidant <i>Lactobacillus</i> |
| url | https://www.mdpi.com/2075-1729/15/1/89 |
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