Potential Modulatory Role of Phoenixin-14 in Epithelial–Mesenchymal Transition of Endometriotic 12Z Cells
<b>Background/Objectives</b>: Endometriosis is a painful chronic condition in which the endometrium grows outside the uterus. The epithelial–mesenchymal transition (EMT) is critical to endometriosis progression, where cells lose epithelial traits and gain invasiveness. <b>Methods&l...
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2025-01-01
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| author | Karolina Iwona Kulinska Magdalena Wierzbicka Anna Dera-Szymanowska Krzysztof Szymanowski Mirosław Andrusiewicz Maria Wołuń-Cholewa |
| author_facet | Karolina Iwona Kulinska Magdalena Wierzbicka Anna Dera-Szymanowska Krzysztof Szymanowski Mirosław Andrusiewicz Maria Wołuń-Cholewa |
| author_sort | Karolina Iwona Kulinska |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Endometriosis is a painful chronic condition in which the endometrium grows outside the uterus. The epithelial–mesenchymal transition (EMT) is critical to endometriosis progression, where cells lose epithelial traits and gain invasiveness. <b>Methods</b>: This study investigates the effects of phoenixin-14 (PNX-14), a neuropeptide found at reduced levels in endometriosis patients, on the expression of two molecular EMT markers, CDH1 (E-cadherin) and THBS2 (thrombospondin 2), as well as cell viability in the endometriosis-derived 12Z cell line. Cells were treated with physiological (0.2 nM) and endometriosis-relevant (0.05 nM) concentrations of PNX-14. Gene expression was analyzed using RT-qPCR, while protein localization was assessed by immunocytochemistry. Cell viability was measured using an XTT assay. <b>Results</b>: <i>THBS2</i> gene expression was significantly decreased, and <i>CDH1</i> remained unchanged in cells stimulated by 0.05 nM PNX-14. Immunolocalization indicates a weaker THBS2 and CDH1 protein immunosignal reaction for 0.05 nM PNX-14. PNX-14 treatment also exhibited a biphasic effect on cell viability. Lower concentration initially decreased viability at 48 h but then significantly increased it at 72 h. This increase coincided with the decrease in <i>THBS2</i> expression, suggesting a potential link between PNX-14, <i>THBS2</i>, and cell viability. <b>Conclusions</b>: A negative correlation between cell viability and the expression of both EMT markers further highlights their possible involvement in the survival and adaptability of ectopic epithelial cells. Our findings suggest a complex interplay between PNX-14, EMT markers, and cell viability in ectopic epithelial cells. PNX-14’s ability to modulate these factors warrants further investigation to elucidate its role in endometriosis. |
| format | Article |
| id | doaj-art-330752bc34ae4ac28ee51867927ac5a8 |
| institution | Kabale University |
| issn | 2227-9059 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-330752bc34ae4ac28ee51867927ac5a82025-01-24T13:24:13ZengMDPI AGBiomedicines2227-90592025-01-0113115810.3390/biomedicines13010158Potential Modulatory Role of Phoenixin-14 in Epithelial–Mesenchymal Transition of Endometriotic 12Z CellsKarolina Iwona Kulinska0Magdalena Wierzbicka1Anna Dera-Szymanowska2Krzysztof Szymanowski3Mirosław Andrusiewicz4Maria Wołuń-Cholewa5Department of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, PolandDepartment of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, PolandClinic of Perinatology and Gynaecology, Poznan University of Medical Sciences, 60-535 Poznań, PolandClinic of Perinatology and Gynaecology, Poznan University of Medical Sciences, 60-535 Poznań, PolandDepartment of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, PolandDepartment of Cell Biology, Poznan University of Medical Sciences, 60-806 Poznań, Poland<b>Background/Objectives</b>: Endometriosis is a painful chronic condition in which the endometrium grows outside the uterus. The epithelial–mesenchymal transition (EMT) is critical to endometriosis progression, where cells lose epithelial traits and gain invasiveness. <b>Methods</b>: This study investigates the effects of phoenixin-14 (PNX-14), a neuropeptide found at reduced levels in endometriosis patients, on the expression of two molecular EMT markers, CDH1 (E-cadherin) and THBS2 (thrombospondin 2), as well as cell viability in the endometriosis-derived 12Z cell line. Cells were treated with physiological (0.2 nM) and endometriosis-relevant (0.05 nM) concentrations of PNX-14. Gene expression was analyzed using RT-qPCR, while protein localization was assessed by immunocytochemistry. Cell viability was measured using an XTT assay. <b>Results</b>: <i>THBS2</i> gene expression was significantly decreased, and <i>CDH1</i> remained unchanged in cells stimulated by 0.05 nM PNX-14. Immunolocalization indicates a weaker THBS2 and CDH1 protein immunosignal reaction for 0.05 nM PNX-14. PNX-14 treatment also exhibited a biphasic effect on cell viability. Lower concentration initially decreased viability at 48 h but then significantly increased it at 72 h. This increase coincided with the decrease in <i>THBS2</i> expression, suggesting a potential link between PNX-14, <i>THBS2</i>, and cell viability. <b>Conclusions</b>: A negative correlation between cell viability and the expression of both EMT markers further highlights their possible involvement in the survival and adaptability of ectopic epithelial cells. Our findings suggest a complex interplay between PNX-14, EMT markers, and cell viability in ectopic epithelial cells. PNX-14’s ability to modulate these factors warrants further investigation to elucidate its role in endometriosis.https://www.mdpi.com/2227-9059/13/1/158endometriosis12Z human ectopic epithelial cell linephoenixin-14 (PNX-14)epithelial cadherin (E-cadherin/<i>CDH1</i>)thrombospondin 2 (<i>THBS2</i>) |
| spellingShingle | Karolina Iwona Kulinska Magdalena Wierzbicka Anna Dera-Szymanowska Krzysztof Szymanowski Mirosław Andrusiewicz Maria Wołuń-Cholewa Potential Modulatory Role of Phoenixin-14 in Epithelial–Mesenchymal Transition of Endometriotic 12Z Cells Biomedicines endometriosis 12Z human ectopic epithelial cell line phoenixin-14 (PNX-14) epithelial cadherin (E-cadherin/<i>CDH1</i>) thrombospondin 2 (<i>THBS2</i>) |
| title | Potential Modulatory Role of Phoenixin-14 in Epithelial–Mesenchymal Transition of Endometriotic 12Z Cells |
| title_full | Potential Modulatory Role of Phoenixin-14 in Epithelial–Mesenchymal Transition of Endometriotic 12Z Cells |
| title_fullStr | Potential Modulatory Role of Phoenixin-14 in Epithelial–Mesenchymal Transition of Endometriotic 12Z Cells |
| title_full_unstemmed | Potential Modulatory Role of Phoenixin-14 in Epithelial–Mesenchymal Transition of Endometriotic 12Z Cells |
| title_short | Potential Modulatory Role of Phoenixin-14 in Epithelial–Mesenchymal Transition of Endometriotic 12Z Cells |
| title_sort | potential modulatory role of phoenixin 14 in epithelial mesenchymal transition of endometriotic 12z cells |
| topic | endometriosis 12Z human ectopic epithelial cell line phoenixin-14 (PNX-14) epithelial cadherin (E-cadherin/<i>CDH1</i>) thrombospondin 2 (<i>THBS2</i>) |
| url | https://www.mdpi.com/2227-9059/13/1/158 |
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