Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency
Pluripotency and self-renewal of embryonic stem cells (ESCs) are marked by core transcription regulators such as Oct4, Sox2, and Nanog. Another important marker of pluripotency is the long noncoding RNA (lncRNA). Here, we ind that a novel long noncoding RNA (lncRNA) Lx8-SINE B2 is a marker of plurip...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/6657597 |
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| _version_ | 1832558376376074240 |
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| author | Fuquan Chen Miao Zhang Xiao Feng Xiaomin Li Haotian Sun Xinyi Lu |
| author_facet | Fuquan Chen Miao Zhang Xiao Feng Xiaomin Li Haotian Sun Xinyi Lu |
| author_sort | Fuquan Chen |
| collection | DOAJ |
| description | Pluripotency and self-renewal of embryonic stem cells (ESCs) are marked by core transcription regulators such as Oct4, Sox2, and Nanog. Another important marker of pluripotency is the long noncoding RNA (lncRNA). Here, we ind that a novel long noncoding RNA (lncRNA) Lx8-SINE B2 is a marker of pluripotency. LncRNA Lx8-SINE B2 is enriched in ESCs and downregulated during ESC differentiation. By rapid amplification of cDNA ends, we identified the full-length sequence of lncRNA Lx8-SINE B2. We further showed that transposable elements at upstream of lncRNA Lx8-SINE B2 could drive the expression of lncRNA Lx8-SINE B2. Furthermore, ESC-specific expression of lncRNA Lx8-SINE B2 was driven by Oct4 and Sox2. In summary, we identified a novel marker lncRNA of ESCs, which is driven by core pluripotency regulators. |
| format | Article |
| id | doaj-art-32e833e182734d3eb22c21af5c3b3b7b |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-32e833e182734d3eb22c21af5c3b3b7b2025-02-03T01:32:26ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/66575976657597Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of PluripotencyFuquan Chen0Miao Zhang1Xiao Feng2Xiaomin Li3Haotian Sun4Xinyi Lu5State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300307, ChinaState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300307, ChinaState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300307, ChinaState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300307, ChinaState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300307, ChinaState Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Nankai University, Tianjin 300307, ChinaPluripotency and self-renewal of embryonic stem cells (ESCs) are marked by core transcription regulators such as Oct4, Sox2, and Nanog. Another important marker of pluripotency is the long noncoding RNA (lncRNA). Here, we ind that a novel long noncoding RNA (lncRNA) Lx8-SINE B2 is a marker of pluripotency. LncRNA Lx8-SINE B2 is enriched in ESCs and downregulated during ESC differentiation. By rapid amplification of cDNA ends, we identified the full-length sequence of lncRNA Lx8-SINE B2. We further showed that transposable elements at upstream of lncRNA Lx8-SINE B2 could drive the expression of lncRNA Lx8-SINE B2. Furthermore, ESC-specific expression of lncRNA Lx8-SINE B2 was driven by Oct4 and Sox2. In summary, we identified a novel marker lncRNA of ESCs, which is driven by core pluripotency regulators.http://dx.doi.org/10.1155/2021/6657597 |
| spellingShingle | Fuquan Chen Miao Zhang Xiao Feng Xiaomin Li Haotian Sun Xinyi Lu Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency Stem Cells International |
| title | Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency |
| title_full | Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency |
| title_fullStr | Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency |
| title_full_unstemmed | Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency |
| title_short | Discovery of a Novel Long Noncoding RNA Lx8-SINE B2 as a Marker of Pluripotency |
| title_sort | discovery of a novel long noncoding rna lx8 sine b2 as a marker of pluripotency |
| url | http://dx.doi.org/10.1155/2021/6657597 |
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