Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in Brazil
Parasitic infections in non-human primates (NHPs) kept ex situ can be caused by zoonotic protists like <i>Balantioides coli</i> and <i>Entamoeba histolytica</i>. In Brazil, little is known about these infections in neotropical species. This study aimed to identify Amoebozoa a...
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2025-01-01
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| author | Laís Dib Breno da Silva Lais Correa Alcides Pissinatti Silvia Moreira Maria Clotilde Tavares Rodrigo Teixeira André Luíz da Costa José Augusto Muniz Amauri Junglos Zelinda Maria Hirano Aline Dada Sidnei da Silva Maria Regina Amendoeira Alynne Barbosa |
| author_facet | Laís Dib Breno da Silva Lais Correa Alcides Pissinatti Silvia Moreira Maria Clotilde Tavares Rodrigo Teixeira André Luíz da Costa José Augusto Muniz Amauri Junglos Zelinda Maria Hirano Aline Dada Sidnei da Silva Maria Regina Amendoeira Alynne Barbosa |
| author_sort | Laís Dib |
| collection | DOAJ |
| description | Parasitic infections in non-human primates (NHPs) kept ex situ can be caused by zoonotic protists like <i>Balantioides coli</i> and <i>Entamoeba histolytica</i>. In Brazil, little is known about these infections in neotropical species. This study aimed to identify Amoebozoa and Ciliophora groups in fecal samples through in vitro isolation and molecular analysis, mapping their distribution in Brazil. Among 511 NHP and 74 handler’s fecal samples, Amoebozoa were found in 61 (11.9%) NHP samples, and Ciliophora in 6 (1.2%). Amoebic cysts were present in 12 (16.2%) human samples. <i>Iodamoeba</i> sp. from <i>S. xanthosternos</i>, <i>E. coli</i> from a handler, and <i>B. coli</i> from <i>P. troglodytes</i> and <i>A. guariba</i> were isolated in vitro. Molecular techniques identified <i>E. dispar</i> (34.2%), <i>E. histolytica</i> (5.1%), <i>E. hartmanni</i> (26.6%), <i>E. coli</i> (15.2%), <i>Iodamoeba</i> sp. (12.6%), <i>E. nana</i> (8.9%), and <i>B. coli</i> (7.6%). Greater protist diversity occurred in northern and southeastern regions, with <i>E. histolytica</i> and <i>B. coli</i> detected in endangered species, such as <i>Saguinus bicolor</i> and <i>Alouatta guariba</i>. Protist overlap between humans and NHPs underscores zoonotic risks. This study presents the first molecular characterization of Amoebozoa and Ciliophora in neotropical NHPs kept ex situ in Brazil, highlighting the need for improved hygiene and management protocols in primate institutions. |
| format | Article |
| id | doaj-art-32a48f0668b64b5babc4f2388ba7c913 |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-32a48f0668b64b5babc4f2388ba7c9132025-01-24T13:44:45ZengMDPI AGPathogens2076-08172025-01-011415610.3390/pathogens14010056Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in BrazilLaís Dib0Breno da Silva1Lais Correa2Alcides Pissinatti3Silvia Moreira4Maria Clotilde Tavares5Rodrigo Teixeira6André Luíz da Costa7José Augusto Muniz8Amauri Junglos9Zelinda Maria Hirano10Aline Dada11Sidnei da Silva12Maria Regina Amendoeira13Alynne Barbosa14Laboratory of Protozoology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, RJ, BrazilLaboratory of Parasitology, Fluminense Federal University, Alameda Barros Terra Blvd., no number, Niterói 24020-150, RJ, BrazilLaboratory of Parasitology, Fluminense Federal University, Alameda Barros Terra Blvd., no number, Niterói 24020-150, RJ, BrazilRio de Janeiro Primatology Center—CPRJ/INEA, Paraíso St., no number, Guapimirim 25940-000, RJ, BrazilRio de Janeiro Primatology Center—CPRJ/INEA, Paraíso St., no number, Guapimirim 25940-000, RJ, BrazilPrimatology Center, University of Brasília, Park Way, no number, Brasília 71750-000, DF, BrazilQuinzinho de Barros Municipal Zoo (Zoo Sorocaba), Teodoro Kaisel St., 883, Sorocaba 18020-268, SP, BrazilQuinzinho de Barros Municipal Zoo (Zoo Sorocaba), Teodoro Kaisel St., 883, Sorocaba 18020-268, SP, BrazilNational Primate Center, BR-316 Hwy., no number, Ananindeua 67033-009, PA, BrazilNational Primate Center, BR-316 Hwy., no number, Ananindeua 67033-009, PA, BrazilBugio Project, Regional University of Blumenau, Rio de Janeiro St., 401, Indaial 89086-000, SC, BrazilBugio Project, Regional University of Blumenau, Rio de Janeiro St., 401, Indaial 89086-000, SC, BrazilEvandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, RJ, BrazilLaboratory of Protozoology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, RJ, BrazilLaboratory of Protozoology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, RJ, BrazilParasitic infections in non-human primates (NHPs) kept ex situ can be caused by zoonotic protists like <i>Balantioides coli</i> and <i>Entamoeba histolytica</i>. In Brazil, little is known about these infections in neotropical species. This study aimed to identify Amoebozoa and Ciliophora groups in fecal samples through in vitro isolation and molecular analysis, mapping their distribution in Brazil. Among 511 NHP and 74 handler’s fecal samples, Amoebozoa were found in 61 (11.9%) NHP samples, and Ciliophora in 6 (1.2%). Amoebic cysts were present in 12 (16.2%) human samples. <i>Iodamoeba</i> sp. from <i>S. xanthosternos</i>, <i>E. coli</i> from a handler, and <i>B. coli</i> from <i>P. troglodytes</i> and <i>A. guariba</i> were isolated in vitro. Molecular techniques identified <i>E. dispar</i> (34.2%), <i>E. histolytica</i> (5.1%), <i>E. hartmanni</i> (26.6%), <i>E. coli</i> (15.2%), <i>Iodamoeba</i> sp. (12.6%), <i>E. nana</i> (8.9%), and <i>B. coli</i> (7.6%). Greater protist diversity occurred in northern and southeastern regions, with <i>E. histolytica</i> and <i>B. coli</i> detected in endangered species, such as <i>Saguinus bicolor</i> and <i>Alouatta guariba</i>. Protist overlap between humans and NHPs underscores zoonotic risks. This study presents the first molecular characterization of Amoebozoa and Ciliophora in neotropical NHPs kept ex situ in Brazil, highlighting the need for improved hygiene and management protocols in primate institutions.https://www.mdpi.com/2076-0817/14/1/56protistszoonosisin vitro isolationmolecular characterization |
| spellingShingle | Laís Dib Breno da Silva Lais Correa Alcides Pissinatti Silvia Moreira Maria Clotilde Tavares Rodrigo Teixeira André Luíz da Costa José Augusto Muniz Amauri Junglos Zelinda Maria Hirano Aline Dada Sidnei da Silva Maria Regina Amendoeira Alynne Barbosa Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in Brazil Pathogens protists zoonosis in vitro isolation molecular characterization |
| title | Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in Brazil |
| title_full | Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in Brazil |
| title_fullStr | Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in Brazil |
| title_full_unstemmed | Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in Brazil |
| title_short | Diversity of the Amoebozoa and Ciliophora Groups in Non-Human Primates Kept Ex Situ and in Their Handlers in Different Institutions in Brazil |
| title_sort | diversity of the amoebozoa and ciliophora groups in non human primates kept ex situ and in their handlers in different institutions in brazil |
| topic | protists zoonosis in vitro isolation molecular characterization |
| url | https://www.mdpi.com/2076-0817/14/1/56 |
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