Interferon-gamma: A Potent Antiviral Agent Targeting Macrophages Infected with LP-BM5 Murine Leukemia Virus, the Causative Agent of 'AIDS' in Mice
Cells of the monocyte/macrophage lineage (MM cells) are known to be infected by retroviruses, including the human immunodeficiency virus (HIV), without cytopathic changes and may serve as a persistent reservoir for the virus during the development of immunodeficiency disease. LP-BM5 murine leukemia...
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| Format: | Article |
| Language: | English |
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Wiley
1992-01-01
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| Series: | Canadian Journal of Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/1992/460394 |
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| _version_ | 1832546518580592640 |
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| author | Jens J Kort Julie L Eiseman |
| author_facet | Jens J Kort Julie L Eiseman |
| author_sort | Jens J Kort |
| collection | DOAJ |
| description | Cells of the monocyte/macrophage lineage (MM cells) are known to be
infected by retroviruses, including the human immunodeficiency virus (HIV), without cytopathic changes
and may serve as a persistent reservoir for the virus during the development of immunodeficiency disease.
LP-BM5 murine leukemia virus (MuLV) infection of C57BL/6 mice and cell lines has been used to optimize
therapy directed against macrophages. Findings in this murine system may be applicable to HN infection
in humans. The effect of recombinant murine interferon-gamma (IFN-γ) and 3' -azido-2',3' -dideoxythymidine
(AZT) as single agents or in combination was investigated in both LP-BM5 MuLV de novo infection and
chronic infection of macrophages. Results indicate that the therapeutic effects of these single agents were
dose-dependent and both agents were similarly effective in reducing the production of infectious virus
determined by XC-plaque assay and by measurements of reverse transcriptase activity in culture supernatants;
and AZT and IFN-γ reduced the production of virus proteins, quantified by laser densitometry of
fluorographs from immunoprecipitated viral proteins using virus-specific antiserum. A combination of IFN-γ
and that AZT showed greater antiviral activity in both LP-BM5 MuLV de novo and chronic infection of
macrophages than either agent alone, suggesting that IFN-γ and AZT represent a potent combination of
antiviral agents targeting macrophages. Further, since a lower concentration of each agent was required
for efficacy in combination therapy, toxicity associated with single agent therapy may be avoided. |
| format | Article |
| id | doaj-art-32868927bc354458a39974e7d897f53e |
| institution | Kabale University |
| issn | 1180-2332 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases |
| spelling | doaj-art-32868927bc354458a39974e7d897f53e2025-02-03T06:48:35ZengWileyCanadian Journal of Infectious Diseases1180-23321992-01-013Suppl B11512210.1155/1992/460394Interferon-gamma: A Potent Antiviral Agent Targeting Macrophages Infected with LP-BM5 Murine Leukemia Virus, the Causative Agent of 'AIDS' in MiceJens J KortJulie L EisemanCells of the monocyte/macrophage lineage (MM cells) are known to be infected by retroviruses, including the human immunodeficiency virus (HIV), without cytopathic changes and may serve as a persistent reservoir for the virus during the development of immunodeficiency disease. LP-BM5 murine leukemia virus (MuLV) infection of C57BL/6 mice and cell lines has been used to optimize therapy directed against macrophages. Findings in this murine system may be applicable to HN infection in humans. The effect of recombinant murine interferon-gamma (IFN-γ) and 3' -azido-2',3' -dideoxythymidine (AZT) as single agents or in combination was investigated in both LP-BM5 MuLV de novo infection and chronic infection of macrophages. Results indicate that the therapeutic effects of these single agents were dose-dependent and both agents were similarly effective in reducing the production of infectious virus determined by XC-plaque assay and by measurements of reverse transcriptase activity in culture supernatants; and AZT and IFN-γ reduced the production of virus proteins, quantified by laser densitometry of fluorographs from immunoprecipitated viral proteins using virus-specific antiserum. A combination of IFN-γ and that AZT showed greater antiviral activity in both LP-BM5 MuLV de novo and chronic infection of macrophages than either agent alone, suggesting that IFN-γ and AZT represent a potent combination of antiviral agents targeting macrophages. Further, since a lower concentration of each agent was required for efficacy in combination therapy, toxicity associated with single agent therapy may be avoided.http://dx.doi.org/10.1155/1992/460394 |
| spellingShingle | Jens J Kort Julie L Eiseman Interferon-gamma: A Potent Antiviral Agent Targeting Macrophages Infected with LP-BM5 Murine Leukemia Virus, the Causative Agent of 'AIDS' in Mice Canadian Journal of Infectious Diseases |
| title | Interferon-gamma: A Potent Antiviral Agent Targeting Macrophages Infected with LP-BM5 Murine Leukemia Virus, the Causative Agent of 'AIDS' in Mice |
| title_full | Interferon-gamma: A Potent Antiviral Agent Targeting Macrophages Infected with LP-BM5 Murine Leukemia Virus, the Causative Agent of 'AIDS' in Mice |
| title_fullStr | Interferon-gamma: A Potent Antiviral Agent Targeting Macrophages Infected with LP-BM5 Murine Leukemia Virus, the Causative Agent of 'AIDS' in Mice |
| title_full_unstemmed | Interferon-gamma: A Potent Antiviral Agent Targeting Macrophages Infected with LP-BM5 Murine Leukemia Virus, the Causative Agent of 'AIDS' in Mice |
| title_short | Interferon-gamma: A Potent Antiviral Agent Targeting Macrophages Infected with LP-BM5 Murine Leukemia Virus, the Causative Agent of 'AIDS' in Mice |
| title_sort | interferon gamma a potent antiviral agent targeting macrophages infected with lp bm5 murine leukemia virus the causative agent of aids in mice |
| url | http://dx.doi.org/10.1155/1992/460394 |
| work_keys_str_mv | AT jensjkort interferongammaapotentantiviralagenttargetingmacrophagesinfectedwithlpbm5murineleukemiavirusthecausativeagentofaidsinmice AT julieleiseman interferongammaapotentantiviralagenttargetingmacrophagesinfectedwithlpbm5murineleukemiavirusthecausativeagentofaidsinmice |