In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions
<b>Aim: </b>This study aimed to show the effects of glutathione, recognized by its antioxidant specialties, on the potential DNA damage (8-hydroxy-2- deoxyguanosine) and the antioxidant system changes upon its implementation in BHK-21 cells cultured with high glucose.<p> <b>M...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
2022-12-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1409 |
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| author | Fatmagul Yur Semiha Dede Sedat Cetin Mehmet Taspinar Ayse Usta |
| author_facet | Fatmagul Yur Semiha Dede Sedat Cetin Mehmet Taspinar Ayse Usta |
| author_sort | Fatmagul Yur |
| collection | DOAJ |
| description | <b>Aim: </b>This study aimed to show the effects of glutathione, recognized by
its antioxidant specialties, on the potential DNA damage (8-hydroxy-2-
deoxyguanosine) and the antioxidant system changes upon its implementation
in BHK-21 cells cultured with high glucose.<p>
<b>Materials and Methods: </b>BHK-21 cell line was regularly surpassed in vitro
conditions (5% FBS, 10% horse serum, 1% L-Glutamine, 1% penicillin/
streptomycin in RPMI 1640 medium, and 5% CO2 and 95% humidity and
37ºC) incubated. The control group determined glucose's IC50 value based on
the viability tests executed on MTT cells. Cells were seeded in plates as each
would have 2x106 cells. The control, the test, and the crossbreed test (glucose;
(285 mM), glutathione (250 µ
,M)) groups were prepared. After 24 hours of
incubation, trypsinized cells were designed for analysis through vitrification.
In the lysate of the cell culture that was procured, Oxidative DNA damage, TAS,
TSO, and OSI were measured by the spectrophotometric system with ELISA.<p>
<b>Results: </b>It was observed that 8-OHdG levels increased significantly with glucose
application. Moreover, the increase in the HG+GSH group was more significant when
compared to the control group (p≤0.05). No difference with the control group was
found only in the group where GSH was applied. As for TAS, whereas any difference was
observed in GSH used groups, the increase in the HG+GSH group was significant compared
to the control group (p≤0.05). that were the same as the control group. TOS and OSI
considerably increased in HG + GSH implemented groups as to the control group (p≤0.05).<p>
<b>Conclusion: </b>According to the results, no protective impacts of glutathione at
the cellular level in the doses mentioned above were observed on high-dose
glucose implemented cells. On the other hand, it was revealed that the applied
amounts of glutathione in the process did not cause any toxic effects. |
| format | Article |
| id | doaj-art-326f29bd9ef341b7b167c960604d0941 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-326f29bd9ef341b7b167c960604d09412025-02-03T11:25:37ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532022-12-013842422471409In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditionsFatmagul Yur0Semiha Dede1Sedat Cetin2Mehmet Taspinar3Ayse Usta4Mugla Sıtkı Kocam University, Fethiye Faculty of Health Sciences, Department of Nutrition and Dietetics, Mugla, TurkeyVan Yuzuncu Yil University, Veterinary Faculty, Department of Biochemistry, Van, TurkeyAnkara Yildirim Beyazıt University, Vocational School of Health Services, Department of Veterinary Medicine Laboratory and Veterinary Health Program, Ankara, TurkeyAksaray University, Medicine Faculty, Department of Medical Biology, Aksaray, TurkeyVan Yuzuncu Yil University, Science Faculty, Department of Chemistry, Van, Turkey<b>Aim: </b>This study aimed to show the effects of glutathione, recognized by its antioxidant specialties, on the potential DNA damage (8-hydroxy-2- deoxyguanosine) and the antioxidant system changes upon its implementation in BHK-21 cells cultured with high glucose.<p> <b>Materials and Methods: </b>BHK-21 cell line was regularly surpassed in vitro conditions (5% FBS, 10% horse serum, 1% L-Glutamine, 1% penicillin/ streptomycin in RPMI 1640 medium, and 5% CO2 and 95% humidity and 37ºC) incubated. The control group determined glucose's IC50 value based on the viability tests executed on MTT cells. Cells were seeded in plates as each would have 2x106 cells. The control, the test, and the crossbreed test (glucose; (285 mM), glutathione (250 µ ,M)) groups were prepared. After 24 hours of incubation, trypsinized cells were designed for analysis through vitrification. In the lysate of the cell culture that was procured, Oxidative DNA damage, TAS, TSO, and OSI were measured by the spectrophotometric system with ELISA.<p> <b>Results: </b>It was observed that 8-OHdG levels increased significantly with glucose application. Moreover, the increase in the HG+GSH group was more significant when compared to the control group (p≤0.05). No difference with the control group was found only in the group where GSH was applied. As for TAS, whereas any difference was observed in GSH used groups, the increase in the HG+GSH group was significant compared to the control group (p≤0.05). that were the same as the control group. TOS and OSI considerably increased in HG + GSH implemented groups as to the control group (p≤0.05).<p> <b>Conclusion: </b>According to the results, no protective impacts of glutathione at the cellular level in the doses mentioned above were observed on high-dose glucose implemented cells. On the other hand, it was revealed that the applied amounts of glutathione in the process did not cause any toxic effects.http://eurasianjvetsci.org/pdf.php3?id=1409dna damageglutathionecell culturetastos |
| spellingShingle | Fatmagul Yur Semiha Dede Sedat Cetin Mehmet Taspinar Ayse Usta In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions Eurasian Journal of Veterinary Sciences dna damage glutathione cell culture tas tos |
| title | In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions |
| title_full | In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions |
| title_fullStr | In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions |
| title_full_unstemmed | In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions |
| title_short | In vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions |
| title_sort | in vitro evaluation of glutathione implementation on oxidative dna damage and oxidant status in high glucose conditions |
| topic | dna damage glutathione cell culture tas tos |
| url | http://eurasianjvetsci.org/pdf.php3?id=1409 |
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