CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand
Background: Currently, antimicrobial agents are widely used in both animals and agriculture, causing the crisis of multidrug-resistant (MDR) bacteria. In this study we surveyed for 4 important antimicrobial-resistant bacteria: extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, Klebsiel...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Journal of Infection and Public Health |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1876034124003587 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832592777621274624 |
|---|---|
| author | Arpasiri Srisrattakarn Bhanubong Saiboonjan Patcharaporn Tippayawat Sunpetch Angkititrakul Aroonwadee Chanawong Chanakan Pornchoo Chokdee Smithkittipol Aroonlug Lulitanond |
| author_facet | Arpasiri Srisrattakarn Bhanubong Saiboonjan Patcharaporn Tippayawat Sunpetch Angkititrakul Aroonwadee Chanawong Chanakan Pornchoo Chokdee Smithkittipol Aroonlug Lulitanond |
| author_sort | Arpasiri Srisrattakarn |
| collection | DOAJ |
| description | Background: Currently, antimicrobial agents are widely used in both animals and agriculture, causing the crisis of multidrug-resistant (MDR) bacteria. In this study we surveyed for 4 important antimicrobial-resistant bacteria: extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, Klebsiella pneumoniae and Salmonella spp., and methicillin-resistant Staphylococcus aureus (MRSA) from the environment around chicken and pig farms. Methods: Forty-four soils, 44 water and 15 bootstrap samples were collected from upstream and downstream of the riverside close to 22 animal farms in 2020 (9 farms) and 2023 (13 farms). The samples were cultured in appropriate media and isolated colonies were further identified. Antimicrobial susceptibility and ESBL production were tested by disk diffusion method except for colistin susceptibility determined by broth disk elution test. Common resistance genes were tested by polymerase chain reaction (PCR) methods. Results: A total of 123 E. coli (n = 59) and Klebsiella species (n = 64) were identified and 25 isolates of them were MDR; 21 and 4 isolates from the downstream and upstream areas respectively. In addition, 13 isolates were ESBL producers, of which 12 isolates were from the downstream area. The most common ESBL gene among both organisms was blaCTX-M, whereas colistin resistance gene, mcr-1, was found in 2 E. coli isolates. Salmonella spp. and MRSA were not isolated from any sample. Conclusions: Antimicrobial-resistant bacteria were found in the environment around the animal farms especially from the downstream area, suggesting the urgent need of judicious use of antimicrobial agents in animal farms. |
| format | Article |
| id | doaj-art-326a6644dfa9477186f2430550a4abcc |
| institution | Kabale University |
| issn | 1876-0341 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Infection and Public Health |
| spelling | doaj-art-326a6644dfa9477186f2430550a4abcc2025-01-21T04:12:55ZengElsevierJournal of Infection and Public Health1876-03412025-02-01182102624CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in ThailandArpasiri Srisrattakarn0Bhanubong Saiboonjan1Patcharaporn Tippayawat2Sunpetch Angkititrakul3Aroonwadee Chanawong4Chanakan Pornchoo5Chokdee Smithkittipol6Aroonlug Lulitanond7Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandCenter for Innovation and Standard for Medical Technology and Physical Therapy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandDivision of Veterinary Public Health, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, ThailandFood System, World Animal Protection (Thailand), ThailandCentre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand; Corresponding author.Background: Currently, antimicrobial agents are widely used in both animals and agriculture, causing the crisis of multidrug-resistant (MDR) bacteria. In this study we surveyed for 4 important antimicrobial-resistant bacteria: extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, Klebsiella pneumoniae and Salmonella spp., and methicillin-resistant Staphylococcus aureus (MRSA) from the environment around chicken and pig farms. Methods: Forty-four soils, 44 water and 15 bootstrap samples were collected from upstream and downstream of the riverside close to 22 animal farms in 2020 (9 farms) and 2023 (13 farms). The samples were cultured in appropriate media and isolated colonies were further identified. Antimicrobial susceptibility and ESBL production were tested by disk diffusion method except for colistin susceptibility determined by broth disk elution test. Common resistance genes were tested by polymerase chain reaction (PCR) methods. Results: A total of 123 E. coli (n = 59) and Klebsiella species (n = 64) were identified and 25 isolates of them were MDR; 21 and 4 isolates from the downstream and upstream areas respectively. In addition, 13 isolates were ESBL producers, of which 12 isolates were from the downstream area. The most common ESBL gene among both organisms was blaCTX-M, whereas colistin resistance gene, mcr-1, was found in 2 E. coli isolates. Salmonella spp. and MRSA were not isolated from any sample. Conclusions: Antimicrobial-resistant bacteria were found in the environment around the animal farms especially from the downstream area, suggesting the urgent need of judicious use of antimicrobial agents in animal farms.http://www.sciencedirect.com/science/article/pii/S1876034124003587Chicken and pig farmsDownstreamEnvironmentMultidrug-resistant (MDR) bacteriaUpstream |
| spellingShingle | Arpasiri Srisrattakarn Bhanubong Saiboonjan Patcharaporn Tippayawat Sunpetch Angkititrakul Aroonwadee Chanawong Chanakan Pornchoo Chokdee Smithkittipol Aroonlug Lulitanond CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand Journal of Infection and Public Health Chicken and pig farms Downstream Environment Multidrug-resistant (MDR) bacteria Upstream |
| title | CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand |
| title_full | CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand |
| title_fullStr | CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand |
| title_full_unstemmed | CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand |
| title_short | CTX-M, SHV, TEM and VEB β-lactamases, and MCR-1 among multidrug-resistant Escherichia coli and Klebsiella isolates from environment near animal farms in Thailand |
| title_sort | ctx m shv tem and veb β lactamases and mcr 1 among multidrug resistant escherichia coli and klebsiella isolates from environment near animal farms in thailand |
| topic | Chicken and pig farms Downstream Environment Multidrug-resistant (MDR) bacteria Upstream |
| url | http://www.sciencedirect.com/science/article/pii/S1876034124003587 |
| work_keys_str_mv | AT arpasirisrisrattakarn ctxmshvtemandvebblactamasesandmcr1amongmultidrugresistantescherichiacoliandklebsiellaisolatesfromenvironmentnearanimalfarmsinthailand AT bhanubongsaiboonjan ctxmshvtemandvebblactamasesandmcr1amongmultidrugresistantescherichiacoliandklebsiellaisolatesfromenvironmentnearanimalfarmsinthailand AT patcharaporntippayawat ctxmshvtemandvebblactamasesandmcr1amongmultidrugresistantescherichiacoliandklebsiellaisolatesfromenvironmentnearanimalfarmsinthailand AT sunpetchangkititrakul ctxmshvtemandvebblactamasesandmcr1amongmultidrugresistantescherichiacoliandklebsiellaisolatesfromenvironmentnearanimalfarmsinthailand AT aroonwadeechanawong ctxmshvtemandvebblactamasesandmcr1amongmultidrugresistantescherichiacoliandklebsiellaisolatesfromenvironmentnearanimalfarmsinthailand AT chanakanpornchoo ctxmshvtemandvebblactamasesandmcr1amongmultidrugresistantescherichiacoliandklebsiellaisolatesfromenvironmentnearanimalfarmsinthailand AT chokdeesmithkittipol ctxmshvtemandvebblactamasesandmcr1amongmultidrugresistantescherichiacoliandklebsiellaisolatesfromenvironmentnearanimalfarmsinthailand AT aroonluglulitanond ctxmshvtemandvebblactamasesandmcr1amongmultidrugresistantescherichiacoliandklebsiellaisolatesfromenvironmentnearanimalfarmsinthailand |