Increased Expression of VEGF and CD31 in Postradiation Rectal Tissue: Implications for Radiation Proctitis
Background. Inflammation mediators related to radiation proctitis are partially elucidated, and neovascularization is thought to play a key role. Objectives. To investigate the expression of vascular endothelial growth factor (VEGF) and CD31 as angiogenetic markers in postradiation rectal tissue. Me...
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| Format: | Article |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/515048 |
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| author | G. Karamanolis I. Delladetsima V. Kouloulias K. Papaxoinis I. Panayiotides D. Haldeopoulos K. Triantafyllou N. Kelekis S. D. Ladas |
| author_facet | G. Karamanolis I. Delladetsima V. Kouloulias K. Papaxoinis I. Panayiotides D. Haldeopoulos K. Triantafyllou N. Kelekis S. D. Ladas |
| author_sort | G. Karamanolis |
| collection | DOAJ |
| description | Background. Inflammation mediators related to radiation proctitis are partially elucidated, and neovascularization is thought to play a key role. Objectives. To investigate the expression of vascular endothelial growth factor (VEGF) and CD31 as angiogenetic markers in postradiation rectal tissue. Methods. Rectal mucosa biopsies from 11 patients who underwent irradiation for prostate cancer were examined immunohistochemically for the expression of VEGF and CD31 at three time settings—before, at the completion of, and 6 months after radiotherapy. VEGF expressing vascular endothelial cells and CD31 expressing microvessels were counted separately in 10 high-power fields (HPFs). VEGF vascular index (VEGF-VI) and microvascular density (MVD) were calculated as the mean number of VEGF positive cells per vessel or the mean number of vessels per HPF, respectively. Histological features were also evaluated. Results. VEGF-VI was significantly higher at the completion of radiotherapy (0.17±0.15 versus 0.41±0.24, P=0.001) declining 6 months after. MVD increased significantly only 6 months after radiotherapy (7.3±3.2 versus 10.5±3.1, P<0.005). The histopathological examination revealed inflammatory changes at the completion of radiotherapy regressing in the majority of cases 6 months after. Conclusions. Our results showed that in postradiation rectal biopsy specimens neoangiogenesis seems to be inflammation-related and constitutes a significant postradiation component of the tissue injury. |
| format | Article |
| id | doaj-art-3238cf210da24acd8d82e901b2e46e54 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-3238cf210da24acd8d82e901b2e46e542025-02-03T06:07:10ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/515048515048Increased Expression of VEGF and CD31 in Postradiation Rectal Tissue: Implications for Radiation ProctitisG. Karamanolis0I. Delladetsima1V. Kouloulias2K. Papaxoinis3I. Panayiotides4D. Haldeopoulos5K. Triantafyllou6N. Kelekis7S. D. Ladas8Hepatogastroenterology Unit, 1st Department of Internal Medicine—Propaedeutic, “Laikon” General Hospital, Athens Medical School, 75 Micras Asias Street, Goudi, 11527 Athens, Greece1st Pathology Laboratory, Athens Medical School, 75 Micras Asias Street, Goudi, 11527 Athens, GreeceRadiotherapy Unit, 2nd Radiology Department, “Attikon” University General Hospital, Athens Medical School, Rimini 1, Xaidari, 12462 Athens, GreeceHepatogastroenterology Unit, 1st Department of Internal Medicine—Propaedeutic, “Laikon” General Hospital, Athens Medical School, 75 Micras Asias Street, Goudi, 11527 Athens, Greece2nd Pathology Laboratory, “Attikon” University General Hospital, Athens Medical School, Rimini 1, Xaidari, 12462 Athens, GreeceRadiotherapy Unit, 2nd Radiology Department, “Attikon” University General Hospital, Athens Medical School, Rimini 1, Xaidari, 12462 Athens, Greece2nd Pathology Laboratory, “Attikon” University General Hospital, Athens Medical School, Rimini 1, Xaidari, 12462 Athens, GreeceRadiotherapy Unit, 2nd Radiology Department, “Attikon” University General Hospital, Athens Medical School, Rimini 1, Xaidari, 12462 Athens, GreeceHepatogastroenterology Unit, 1st Department of Internal Medicine—Propaedeutic, “Laikon” General Hospital, Athens Medical School, 75 Micras Asias Street, Goudi, 11527 Athens, GreeceBackground. Inflammation mediators related to radiation proctitis are partially elucidated, and neovascularization is thought to play a key role. Objectives. To investigate the expression of vascular endothelial growth factor (VEGF) and CD31 as angiogenetic markers in postradiation rectal tissue. Methods. Rectal mucosa biopsies from 11 patients who underwent irradiation for prostate cancer were examined immunohistochemically for the expression of VEGF and CD31 at three time settings—before, at the completion of, and 6 months after radiotherapy. VEGF expressing vascular endothelial cells and CD31 expressing microvessels were counted separately in 10 high-power fields (HPFs). VEGF vascular index (VEGF-VI) and microvascular density (MVD) were calculated as the mean number of VEGF positive cells per vessel or the mean number of vessels per HPF, respectively. Histological features were also evaluated. Results. VEGF-VI was significantly higher at the completion of radiotherapy (0.17±0.15 versus 0.41±0.24, P=0.001) declining 6 months after. MVD increased significantly only 6 months after radiotherapy (7.3±3.2 versus 10.5±3.1, P<0.005). The histopathological examination revealed inflammatory changes at the completion of radiotherapy regressing in the majority of cases 6 months after. Conclusions. Our results showed that in postradiation rectal biopsy specimens neoangiogenesis seems to be inflammation-related and constitutes a significant postradiation component of the tissue injury.http://dx.doi.org/10.1155/2013/515048 |
| spellingShingle | G. Karamanolis I. Delladetsima V. Kouloulias K. Papaxoinis I. Panayiotides D. Haldeopoulos K. Triantafyllou N. Kelekis S. D. Ladas Increased Expression of VEGF and CD31 in Postradiation Rectal Tissue: Implications for Radiation Proctitis Mediators of Inflammation |
| title | Increased Expression of VEGF and CD31 in Postradiation Rectal Tissue: Implications for Radiation Proctitis |
| title_full | Increased Expression of VEGF and CD31 in Postradiation Rectal Tissue: Implications for Radiation Proctitis |
| title_fullStr | Increased Expression of VEGF and CD31 in Postradiation Rectal Tissue: Implications for Radiation Proctitis |
| title_full_unstemmed | Increased Expression of VEGF and CD31 in Postradiation Rectal Tissue: Implications for Radiation Proctitis |
| title_short | Increased Expression of VEGF and CD31 in Postradiation Rectal Tissue: Implications for Radiation Proctitis |
| title_sort | increased expression of vegf and cd31 in postradiation rectal tissue implications for radiation proctitis |
| url | http://dx.doi.org/10.1155/2013/515048 |
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