Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance
In this work, we evaluated the effects of administration of OVA on phenotype and function of intraepithelial lymphocytes (IELs) from small intestine of transgenic (TGN) DO11.10 and wild-type BALB/c mice. While the small intestines from BALB/c presented a well preserved structure, those from TGN show...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/208054 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832564676032987136 |
|---|---|
| author | Maristela Ruberti Luis Gustavo Romani Fernandes Patricia Ucelli Simioni Dirce Lima Gabriel Áureo Tatsumi Yamada Wirla Maria da Silva Cunha Tamashiro |
| author_facet | Maristela Ruberti Luis Gustavo Romani Fernandes Patricia Ucelli Simioni Dirce Lima Gabriel Áureo Tatsumi Yamada Wirla Maria da Silva Cunha Tamashiro |
| author_sort | Maristela Ruberti |
| collection | DOAJ |
| description | In this work, we evaluated the effects of administration of OVA on phenotype and function of intraepithelial lymphocytes (IELs) from small intestine of transgenic (TGN) DO11.10 and wild-type BALB/c mice. While the small intestines from BALB/c presented a well preserved structure, those from TGN showed an inflamed aspect. The ingestion of OVA induced a reduction in the number of IELs in small intestines of TGN, but it did not change the frequencies of CD8+ and CD4+ T-cell subsets. Administration of OVA via oral + ip increased the frequency of CD103+ cells in CD4+ T-cell subset in IELs of both BALB/c and TGN mice and elevated its expression in CD8β+ T-cell subset in IELs of TGN. The frequency of Foxp3+ cells increased in all subsets in IELs of BALB/c treated with OVA; in IELs of TGN, it increased only in CD25+ subset. IELs from BALB/c tolerant mice had lower expression of all cytokines studied, whereas those from TGN showed high expression of inflammatory cytokines, especially of IFN-γ, TGF-β, and TNF-α. Overall, our results suggest that the inability of TGN to become tolerant may be related to disorganization and altered proportions of inflammatory/regulatory T cells in its intestinal mucosa. |
| format | Article |
| id | doaj-art-32321f778e6f4e7db2d90a935fd5f6cc |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-32321f778e6f4e7db2d90a935fd5f6cc2025-02-03T01:10:24ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/208054208054Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral ToleranceMaristela Ruberti0Luis Gustavo Romani Fernandes1Patricia Ucelli Simioni2Dirce Lima Gabriel3Áureo Tatsumi Yamada4Wirla Maria da Silva Cunha Tamashiro5Department of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato of 255, CP 6109, 13083-970 Campinas, SP, BrazilDepartment of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato of 255, CP 6109, 13083-970 Campinas, SP, BrazilDepartment of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato of 255, CP 6109, 13083-970 Campinas, SP, BrazilDepartment of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato of 255, CP 6109, 13083-970 Campinas, SP, BrazilDepartment of Histology and Embryology, Institute of Biology, University of Campinas (UNICAMP), CP 6109, 13083-970 Campinas, SP, BrazilDepartment of Genetics, Evolution and Bioagents, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato of 255, CP 6109, 13083-970 Campinas, SP, BrazilIn this work, we evaluated the effects of administration of OVA on phenotype and function of intraepithelial lymphocytes (IELs) from small intestine of transgenic (TGN) DO11.10 and wild-type BALB/c mice. While the small intestines from BALB/c presented a well preserved structure, those from TGN showed an inflamed aspect. The ingestion of OVA induced a reduction in the number of IELs in small intestines of TGN, but it did not change the frequencies of CD8+ and CD4+ T-cell subsets. Administration of OVA via oral + ip increased the frequency of CD103+ cells in CD4+ T-cell subset in IELs of both BALB/c and TGN mice and elevated its expression in CD8β+ T-cell subset in IELs of TGN. The frequency of Foxp3+ cells increased in all subsets in IELs of BALB/c treated with OVA; in IELs of TGN, it increased only in CD25+ subset. IELs from BALB/c tolerant mice had lower expression of all cytokines studied, whereas those from TGN showed high expression of inflammatory cytokines, especially of IFN-γ, TGF-β, and TNF-α. Overall, our results suggest that the inability of TGN to become tolerant may be related to disorganization and altered proportions of inflammatory/regulatory T cells in its intestinal mucosa.http://dx.doi.org/10.1155/2012/208054 |
| spellingShingle | Maristela Ruberti Luis Gustavo Romani Fernandes Patricia Ucelli Simioni Dirce Lima Gabriel Áureo Tatsumi Yamada Wirla Maria da Silva Cunha Tamashiro Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance Clinical and Developmental Immunology |
| title | Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance |
| title_full | Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance |
| title_fullStr | Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance |
| title_full_unstemmed | Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance |
| title_short | Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance |
| title_sort | phenotypical and functional analysis of intraepithelial lymphocytes from small intestine of mice in oral tolerance |
| url | http://dx.doi.org/10.1155/2012/208054 |
| work_keys_str_mv | AT maristelaruberti phenotypicalandfunctionalanalysisofintraepitheliallymphocytesfromsmallintestineofmiceinoraltolerance AT luisgustavoromanifernandes phenotypicalandfunctionalanalysisofintraepitheliallymphocytesfromsmallintestineofmiceinoraltolerance AT patriciaucellisimioni phenotypicalandfunctionalanalysisofintraepitheliallymphocytesfromsmallintestineofmiceinoraltolerance AT dircelimagabriel phenotypicalandfunctionalanalysisofintraepitheliallymphocytesfromsmallintestineofmiceinoraltolerance AT aureotatsumiyamada phenotypicalandfunctionalanalysisofintraepitheliallymphocytesfromsmallintestineofmiceinoraltolerance AT wirlamariadasilvacunhatamashiro phenotypicalandfunctionalanalysisofintraepitheliallymphocytesfromsmallintestineofmiceinoraltolerance |