Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil
Staphylococcus saprophyticus is an important agent of urinary tract infection (UTI) in young women, but information about this pathogen in human microbiota and in common environment is lacking. The aim of this study was to characterize S. saprophyticus isolates from genitoanal microbiota of 621 preg...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/4287547 |
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| author | Viviane Santos de Sousa Ana Paula de Souza da-Silva Leif Sorenson Raphael Paiva Paschoal Renata Fernandes Rabello Eloiza Helena Campana Márcia Soares Pinheiro Lyssa Oliveira Ferreira dos Santos Natacha Martins Ana Carolina Nunes Botelho Renata Cristina Picão Sérgio Eduardo Longo Fracalanzza Lee Woodland Riley George Sensabaugh Beatriz Meurer Moreira |
| author_facet | Viviane Santos de Sousa Ana Paula de Souza da-Silva Leif Sorenson Raphael Paiva Paschoal Renata Fernandes Rabello Eloiza Helena Campana Márcia Soares Pinheiro Lyssa Oliveira Ferreira dos Santos Natacha Martins Ana Carolina Nunes Botelho Renata Cristina Picão Sérgio Eduardo Longo Fracalanzza Lee Woodland Riley George Sensabaugh Beatriz Meurer Moreira |
| author_sort | Viviane Santos de Sousa |
| collection | DOAJ |
| description | Staphylococcus saprophyticus is an important agent of urinary tract infection (UTI) in young women, but information about this pathogen in human microbiota and in common environment is lacking. The aim of this study was to characterize S. saprophyticus isolates from genitoanal microbiota of 621 pregnant women, 10 minas cheese packs, and five beaches in Rio de Janeiro city and compare PFGE profiles of these isolates with five UTI PFGE clusters described in this city. We investigated 65 S. saprophyticus isolates from microbiota, 13 from minas cheese, and 30 from beaches and 32 UTI isolates. Antimicrobial resistance was determined by disk diffusion, MIC by agar dilution, and PCR. Erythromycin-resistance genes erm(C), msr(A), msr(B), mph(C), and lin(A) were found in 93% of isolates. Trimethoprim-sulfamethoxazole resistance correlated with dfrG or dfrA genes. Three cefoxitin-resistant isolates carried the mecA gene. All isolates obtained from cheese were susceptible to all antimicrobial agents. Six of 10 pregnant women with >1 isolate had monoclonal colonization. Isolates from pregnant women shared 100% similarity with UTI PFGE cluster types A and E obtained almost 10 years previously, suggesting temporal persistence of S. saprophyticus. Antimicrobial resistance of beach isolates reflected the profiles of human isolates. Taken together, results indicate a shared source for human and environmental isolates. |
| format | Article |
| id | doaj-art-322acd009484416bb404b0186cbe3e8f |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-322acd009484416bb404b0186cbe3e8f2025-02-03T05:47:53ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/42875474287547Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, BrazilViviane Santos de Sousa0Ana Paula de Souza da-Silva1Leif Sorenson2Raphael Paiva Paschoal3Renata Fernandes Rabello4Eloiza Helena Campana5Márcia Soares Pinheiro6Lyssa Oliveira Ferreira dos Santos7Natacha Martins8Ana Carolina Nunes Botelho9Renata Cristina Picão10Sérgio Eduardo Longo Fracalanzza11Lee Woodland Riley12George Sensabaugh13Beatriz Meurer Moreira14Laboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilLaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilSchool of Public Health, University of California, Berkeley, Berkeley, CA, USALaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilInstituto Biomédico, Universidade Federal Fluminense, Niterói, RJ, BrazilLaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilInstituto Biomédico, Universidade Federal Fluminense, Niterói, RJ, BrazilLaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilLaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilLaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilLaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilLaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilSchool of Public Health, University of California, Berkeley, Berkeley, CA, USASchool of Public Health, University of California, Berkeley, Berkeley, CA, USALaboratório de Investigação em Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, BrazilStaphylococcus saprophyticus is an important agent of urinary tract infection (UTI) in young women, but information about this pathogen in human microbiota and in common environment is lacking. The aim of this study was to characterize S. saprophyticus isolates from genitoanal microbiota of 621 pregnant women, 10 minas cheese packs, and five beaches in Rio de Janeiro city and compare PFGE profiles of these isolates with five UTI PFGE clusters described in this city. We investigated 65 S. saprophyticus isolates from microbiota, 13 from minas cheese, and 30 from beaches and 32 UTI isolates. Antimicrobial resistance was determined by disk diffusion, MIC by agar dilution, and PCR. Erythromycin-resistance genes erm(C), msr(A), msr(B), mph(C), and lin(A) were found in 93% of isolates. Trimethoprim-sulfamethoxazole resistance correlated with dfrG or dfrA genes. Three cefoxitin-resistant isolates carried the mecA gene. All isolates obtained from cheese were susceptible to all antimicrobial agents. Six of 10 pregnant women with >1 isolate had monoclonal colonization. Isolates from pregnant women shared 100% similarity with UTI PFGE cluster types A and E obtained almost 10 years previously, suggesting temporal persistence of S. saprophyticus. Antimicrobial resistance of beach isolates reflected the profiles of human isolates. Taken together, results indicate a shared source for human and environmental isolates.http://dx.doi.org/10.1155/2017/4287547 |
| spellingShingle | Viviane Santos de Sousa Ana Paula de Souza da-Silva Leif Sorenson Raphael Paiva Paschoal Renata Fernandes Rabello Eloiza Helena Campana Márcia Soares Pinheiro Lyssa Oliveira Ferreira dos Santos Natacha Martins Ana Carolina Nunes Botelho Renata Cristina Picão Sérgio Eduardo Longo Fracalanzza Lee Woodland Riley George Sensabaugh Beatriz Meurer Moreira Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil International Journal of Microbiology |
| title | Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil |
| title_full | Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil |
| title_fullStr | Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil |
| title_full_unstemmed | Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil |
| title_short | Staphylococcus saprophyticus Recovered from Humans, Food, and Recreational Waters in Rio de Janeiro, Brazil |
| title_sort | staphylococcus saprophyticus recovered from humans food and recreational waters in rio de janeiro brazil |
| url | http://dx.doi.org/10.1155/2017/4287547 |
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