Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation
DNA vaccination has been developed in the last two decades in human and animal species as a promising alternative to conventional vaccination. It consists in the injection, in the muscle, for example, of plasmid DNA encoding the vaccinating polypeptide. Electroporation which forces the entrance of t...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/326825 |
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| author | Daniel Dory Vincent Le Moigne Roland Cariolet Véronique Béven André Keranflec’h André Jestin |
| author_facet | Daniel Dory Vincent Le Moigne Roland Cariolet Véronique Béven André Keranflec’h André Jestin |
| author_sort | Daniel Dory |
| collection | DOAJ |
| description | DNA vaccination has been developed in the last two decades in human and animal species as a promising alternative to conventional vaccination. It consists in the injection, in the muscle, for example, of plasmid DNA encoding the vaccinating polypeptide. Electroporation which forces the entrance of the plasmid DNA in cells at the injection point has been described as a powerful and promising strategy to enhance DNA vaccine efficacy. Due to the fact that the vaccine is composed of DNA, close attention on the fate of the plasmid DNA upon vaccination has to be taken into account, especially at the injection point. To perform such studies, the muscle injection point has to be precisely recovered and collected several weeks after injection. This is even more difficult for large and growing animals. A technique has been developed to localize precisely and collect efficiently the muscle injection points in growing piglets 6 weeks after DNA vaccination accompanied or not by electroporation. Electroporation did not significantly increase the level of remaining plasmids compared to nonelectroporated piglets, and, in all the cases, the levels were below the limit recommended by the FDA to research integration events of plasmid DNA into the host DNA. |
| format | Article |
| id | doaj-art-322612b29e864a10ab043e2dec12091e |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-322612b29e864a10ab043e2dec12091e2025-02-03T00:59:07ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/326825326825Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without ElectroporationDaniel Dory0Vincent Le Moigne1Roland Cariolet2Véronique Béven3André Keranflec’h4André Jestin5French Agency for Food, Environmental and Occupational Health Safety (ANSES), Viral Genetics and Biosafety Unit, 22440 Ploufragan, FranceFrench Agency for Food, Environmental and Occupational Health Safety (ANSES), Viral Genetics and Biosafety Unit, 22440 Ploufragan, FranceFrench Agency for Food, Environmental and Occupational Health Safety (ANSES), Pathogen-Free Pig Breeding and Testing Facility, 22440 Ploufragan, FranceFrench Agency for Food, Environmental and Occupational Health Safety (ANSES), Viral Genetics and Biosafety Unit, 22440 Ploufragan, FranceFrench Agency for Food, Environmental and Occupational Health Safety (ANSES), Pathogen-Free Pig Breeding and Testing Facility, 22440 Ploufragan, FranceFrench Agency for Food, Environmental and Occupational Health Safety (ANSES), Viral Genetics and Biosafety Unit, 22440 Ploufragan, FranceDNA vaccination has been developed in the last two decades in human and animal species as a promising alternative to conventional vaccination. It consists in the injection, in the muscle, for example, of plasmid DNA encoding the vaccinating polypeptide. Electroporation which forces the entrance of the plasmid DNA in cells at the injection point has been described as a powerful and promising strategy to enhance DNA vaccine efficacy. Due to the fact that the vaccine is composed of DNA, close attention on the fate of the plasmid DNA upon vaccination has to be taken into account, especially at the injection point. To perform such studies, the muscle injection point has to be precisely recovered and collected several weeks after injection. This is even more difficult for large and growing animals. A technique has been developed to localize precisely and collect efficiently the muscle injection points in growing piglets 6 weeks after DNA vaccination accompanied or not by electroporation. Electroporation did not significantly increase the level of remaining plasmids compared to nonelectroporated piglets, and, in all the cases, the levels were below the limit recommended by the FDA to research integration events of plasmid DNA into the host DNA.http://dx.doi.org/10.1155/2015/326825 |
| spellingShingle | Daniel Dory Vincent Le Moigne Roland Cariolet Véronique Béven André Keranflec’h André Jestin Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation Journal of Immunology Research |
| title | Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation |
| title_full | Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation |
| title_fullStr | Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation |
| title_full_unstemmed | Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation |
| title_short | Targeted Collection of Plasmid DNA in Large and Growing Animal Muscles 6 Weeks after DNA Vaccination with and without Electroporation |
| title_sort | targeted collection of plasmid dna in large and growing animal muscles 6 weeks after dna vaccination with and without electroporation |
| url | http://dx.doi.org/10.1155/2015/326825 |
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