Phytochemicals and Antioxidant Activity of Microalgae <I>Dunaliella salina</I> and <I>Botryococcus braunii</I>
Highlight Research • The methanolic extracts of salinaand B. braunii contained alkaloids, steroids, triterpenoids, flavonoids, and saponins. • Chlorophyll a, chlorophyll b, and carotenoids were most abundant in the ethyl acetate extracts of salinaand B. braunii. • The maximum total phenolic con...
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Faculty of Fisheries and Marine Universitas Airlangga
2023-05-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/36740 |
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| author | Ali Ridlo Delianis Pringgenies Ribka Anindita Br Perangin-angin Dafit Ariyanto |
| author_facet | Ali Ridlo Delianis Pringgenies Ribka Anindita Br Perangin-angin Dafit Ariyanto |
| author_sort | Ali Ridlo |
| collection | DOAJ |
| description | Highlight Research
• The methanolic extracts of salinaand B. braunii contained alkaloids, steroids, triterpenoids, flavonoids, and saponins.
• Chlorophyll a, chlorophyll b, and carotenoids were most abundant in the ethyl acetate extracts of salinaand B. braunii.
• The maximum total phenolic content was observed in the n-hexane extract of salina(61.28 mg GAE/g sample) and the ethyl acetate extract of B. braunii (46.94 mg GAE/g sample).
• The n-hexane extracts exhibited the highest antioxidant activity, whereas saponins were most abundant in the methanol extracts.
Abstract
Microalgal species such as Dunaliella salina and Botryococcus braunii are reportedly rich in natural antioxidants and phytochemicals. This study aimed to determine the phytochemicals and the antioxidant activity of D. salina and B. braunii. Microalgal samples were obtained from the Brackish Water Cultivation Fisheries Center (BPBAP), Situbondo, East Java. The extracts were prepared using the multilevel maceration method. The antioxidant activity of the algal species was analyzed using 1,1-diphenyl-2-picrylhydraxyl (DPPH). Quantitative analysis revealed that D. salina and B. braunii contained antioxidants, indicated by the appearance of yellow spots on the purple background of the TLC plate. The n-hexane extract of D. salina exhibited the highest antioxidant activity with a half-maximal inhibitory concentration (IC50) of 443.28 ppm, 61.28 mg GAE/g sample of total phenolics, 0.106 mg/g of chlorophyll a, 0.165 mg/g of chlorophyll b, and 1,697 mol/g of carotenoids. In contrast, the ethyl acetate extract of B. braunii exhibited the highest antioxidant activity with an IC50 of 634.55 ppm, 46.94 mg GAE/g sample of total phenolics, 18.146 mg/g of chlorophyll a, 12.592 mg/g of chlorophyll b, and 4573 mol/g of carotenoids. The microalgal species used in this study exhibited extremely weak antioxidant activity. |
| format | Article |
| id | doaj-art-32249f53ff2845d1a1a373cd7b30c582 |
| institution | Kabale University |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
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| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-32249f53ff2845d1a1a373cd7b30c5822025-02-03T05:30:46ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592023-05-0115243844710.20473/jipk.v15i2.3674034781Phytochemicals and Antioxidant Activity of Microalgae <I>Dunaliella salina</I> and <I>Botryococcus braunii</I>Ali Ridlo0https://orcid.org/0000-0001-8645-115XDelianis Pringgenies1https://orcid.org/0000-0001-7388-0000Ribka Anindita Br Perangin-angin2https://orcid.org/0000-0003-1740-7969Dafit Ariyanto3https://orcid.org/0000-0001-6749-0997Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Semarang, Central Java. IndonesiaDepartment of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Semarang, Central Java. IndonesiaDepartment of Marine Sciences, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Semarang, Central Java. IndonesiaResearch Center for Oceanography, National Research and Innovation Agency, Jakarta. IndonesiaHighlight Research • The methanolic extracts of salinaand B. braunii contained alkaloids, steroids, triterpenoids, flavonoids, and saponins. • Chlorophyll a, chlorophyll b, and carotenoids were most abundant in the ethyl acetate extracts of salinaand B. braunii. • The maximum total phenolic content was observed in the n-hexane extract of salina(61.28 mg GAE/g sample) and the ethyl acetate extract of B. braunii (46.94 mg GAE/g sample). • The n-hexane extracts exhibited the highest antioxidant activity, whereas saponins were most abundant in the methanol extracts. Abstract Microalgal species such as Dunaliella salina and Botryococcus braunii are reportedly rich in natural antioxidants and phytochemicals. This study aimed to determine the phytochemicals and the antioxidant activity of D. salina and B. braunii. Microalgal samples were obtained from the Brackish Water Cultivation Fisheries Center (BPBAP), Situbondo, East Java. The extracts were prepared using the multilevel maceration method. The antioxidant activity of the algal species was analyzed using 1,1-diphenyl-2-picrylhydraxyl (DPPH). Quantitative analysis revealed that D. salina and B. braunii contained antioxidants, indicated by the appearance of yellow spots on the purple background of the TLC plate. The n-hexane extract of D. salina exhibited the highest antioxidant activity with a half-maximal inhibitory concentration (IC50) of 443.28 ppm, 61.28 mg GAE/g sample of total phenolics, 0.106 mg/g of chlorophyll a, 0.165 mg/g of chlorophyll b, and 1,697 mol/g of carotenoids. In contrast, the ethyl acetate extract of B. braunii exhibited the highest antioxidant activity with an IC50 of 634.55 ppm, 46.94 mg GAE/g sample of total phenolics, 18.146 mg/g of chlorophyll a, 12.592 mg/g of chlorophyll b, and 4573 mol/g of carotenoids. The microalgal species used in this study exhibited extremely weak antioxidant activity.https://e-journal.unair.ac.id/JIPK/article/view/36740carotenoidschlorophyllsdpphmicroalgaetotal phenolics |
| spellingShingle | Ali Ridlo Delianis Pringgenies Ribka Anindita Br Perangin-angin Dafit Ariyanto Phytochemicals and Antioxidant Activity of Microalgae <I>Dunaliella salina</I> and <I>Botryococcus braunii</I> Jurnal Ilmiah Perikanan dan Kelautan carotenoids chlorophylls dpph microalgae total phenolics |
| title | Phytochemicals and Antioxidant Activity of Microalgae <I>Dunaliella salina</I> and <I>Botryococcus braunii</I> |
| title_full | Phytochemicals and Antioxidant Activity of Microalgae <I>Dunaliella salina</I> and <I>Botryococcus braunii</I> |
| title_fullStr | Phytochemicals and Antioxidant Activity of Microalgae <I>Dunaliella salina</I> and <I>Botryococcus braunii</I> |
| title_full_unstemmed | Phytochemicals and Antioxidant Activity of Microalgae <I>Dunaliella salina</I> and <I>Botryococcus braunii</I> |
| title_short | Phytochemicals and Antioxidant Activity of Microalgae <I>Dunaliella salina</I> and <I>Botryococcus braunii</I> |
| title_sort | phytochemicals and antioxidant activity of microalgae i dunaliella salina i and i botryococcus braunii i |
| topic | carotenoids chlorophylls dpph microalgae total phenolics |
| url | https://e-journal.unair.ac.id/JIPK/article/view/36740 |
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