Efficacies of corynebacterium pseudotuberculosis vaccines against caseous lymphadenitis in mice and sheep
<b>Aim:</b> This study was aimed to prepare Corynebacterium pseudotuberculosis vaccines against Caseous Lymphadenitis (CLA) and to determine the effectiveness of vaccines in mice and sheep.4<p> <b>Materials and Methods:</b> C. pseudotuberculosis vaccines were prepared i...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1034 |
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| Summary: | <b>Aim:</b> This study was aimed to prepare Corynebacterium pseudotuberculosis
vaccines against Caseous Lymphadenitis (CLA) and
to determine the effectiveness of vaccines in mice and sheep.4<p>
<b>Materials and Methods:</b> C. pseudotuberculosis vaccines were
prepared in 3 different combination (PLD, bacterin and combined).
Vaccines were subcutaneously administered twice (0.1
mL) 3 weeks intervals to mice. After vaccination at 15 days, mice
were subcutaneously challenged with C. pseudotuberculosis and
PLD of the challenge strain with LD50 dose. All mice were observed
throughout 20 days for morbidity and mortality. Internal
organs of deaths/euthanized mice were cultured for re-isolation
of bacteria. Sheep were subcutaneously vaccinated twice (1 mL)
with vaccines of commercial or combined, 3 weeks intervals.
Blood sera were taken before and after vaccination at 45-60 days
intervals. In addition, lymph nodes were examined microscopically
by the presence any abscess. Antibody titers to C. psedotuberculosis
in mice and sheep were determined by modified
ELISA.<p>
<b>Results:</b> When compared to controls, antibody titers were significantly
higher in vaccinated mice (P<0.05). In challenged trials
with PLD, the ratio of morbidity and mortality was not observed
in the vaccines groups PLD and combined. But, the lower ratio
of morbidity and mortality in challenged trials with live bacteria
were determined in the groups of bacterin and combined. When
the levels of antibody in sheep were found to be high, abscess
formation were lower in, comparison to controls.<p>
<b>Conclusions:</b> Especially combined C. pseudotuberculosis vaccines
were concluded to be useful in prevention of CLA in sheep. |
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| ISSN: | 1309-6958 2146-1953 |