Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810
The aim of this study was to investigate the effect of intramammary infusions of natural composition GLP 810 with immunomodulating properties on the local nonspecific cellular and humoral immune response in cows with subclinical mastitis. The composition GLP 810 consists of lactic acid, lysozyme, gl...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/8238029 |
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| author | Gundega Gulbe Māra Pilmane Vaira Saulīte Simona Doniņa Jevgenijs Jermolajevs Lilija Peškova Anda Valdovska |
| author_facet | Gundega Gulbe Māra Pilmane Vaira Saulīte Simona Doniņa Jevgenijs Jermolajevs Lilija Peškova Anda Valdovska |
| author_sort | Gundega Gulbe |
| collection | DOAJ |
| description | The aim of this study was to investigate the effect of intramammary infusions of natural composition GLP 810 with immunomodulating properties on the local nonspecific cellular and humoral immune response in cows with subclinical mastitis. The composition GLP 810 consists of lactic acid, lysozyme, glycopeptides, and 0.9% solution of NaCl. The following parameters were studied: (1) leukocyte differential distribution in milk, (2) expression of cytokines in milk leukocytes, (3) antibacterial activity, and (4) milk quality. Nineteen mammary glands in five lactating cows were infused with 10 mL of GLP 810, and nineteen other glands from five control cows were treated with 10 mL 0.9% NaCl. The results showed that after intramammary administration of the composition GLP 810 three times with 48 h intervals, the following effects on leukocyte populations in milk were observed: (1) an increase in the number of polymorphonuclear leukocytes and lymphocytes and (2) a decrease in the number of macrophages. A reduction in the number of pathogenic bacteria was also detected. The analyses of tumour necrosis factor-alpha, interleukin-10, and beta-defensin-2 revealed that the production of the aforementioned cytokines significantly increased, whereas no significant effects on interleukin-1 and caspase-6 expression in milk leukocytes were recorded. However, there were significant differences between mammary glands with high and low milk somatic cell count. The results suggest that the composition GLP 810 has an immunomodulatory effect on mammary glands and it could be used for improving the immune response in cows with subclinical mastitis during lactation. |
| format | Article |
| id | doaj-art-31e541dd808f437aa272636e60d45be0 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-31e541dd808f437aa272636e60d45be02025-02-03T01:05:06ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/82380298238029Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810Gundega Gulbe0Māra Pilmane1Vaira Saulīte2Simona Doniņa3Jevgenijs Jermolajevs4Lilija Peškova5Anda Valdovska6Riga Stradins University, Institute of Microbiology and Virology, 5 Ratsupites Street, Riga LV-1067, LatviaRiga Stradins University, Faculty of Medicine, Institute of Anatomy and Anthropology, Anatomical Theatre, 9 Kronvalda Bulvaris, Riga LV-1010, LatviaRiga Stradins University, Institute of Microbiology and Virology, 5 Ratsupites Street, Riga LV-1067, LatviaRiga Stradins University, Institute of Microbiology and Virology, 5 Ratsupites Street, Riga LV-1067, LatviaRiga Stradins University, Institute of Microbiology and Virology, 5 Ratsupites Street, Riga LV-1067, LatviaRiga Stradins University, Institute of Microbiology and Virology, 5 Ratsupites Street, Riga LV-1067, LatviaLatvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, 8 Kr. Helmana Street, Jelgava LV-3004, LatviaThe aim of this study was to investigate the effect of intramammary infusions of natural composition GLP 810 with immunomodulating properties on the local nonspecific cellular and humoral immune response in cows with subclinical mastitis. The composition GLP 810 consists of lactic acid, lysozyme, glycopeptides, and 0.9% solution of NaCl. The following parameters were studied: (1) leukocyte differential distribution in milk, (2) expression of cytokines in milk leukocytes, (3) antibacterial activity, and (4) milk quality. Nineteen mammary glands in five lactating cows were infused with 10 mL of GLP 810, and nineteen other glands from five control cows were treated with 10 mL 0.9% NaCl. The results showed that after intramammary administration of the composition GLP 810 three times with 48 h intervals, the following effects on leukocyte populations in milk were observed: (1) an increase in the number of polymorphonuclear leukocytes and lymphocytes and (2) a decrease in the number of macrophages. A reduction in the number of pathogenic bacteria was also detected. The analyses of tumour necrosis factor-alpha, interleukin-10, and beta-defensin-2 revealed that the production of the aforementioned cytokines significantly increased, whereas no significant effects on interleukin-1 and caspase-6 expression in milk leukocytes were recorded. However, there were significant differences between mammary glands with high and low milk somatic cell count. The results suggest that the composition GLP 810 has an immunomodulatory effect on mammary glands and it could be used for improving the immune response in cows with subclinical mastitis during lactation.http://dx.doi.org/10.1155/2020/8238029 |
| spellingShingle | Gundega Gulbe Māra Pilmane Vaira Saulīte Simona Doniņa Jevgenijs Jermolajevs Lilija Peškova Anda Valdovska Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810 Mediators of Inflammation |
| title | Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810 |
| title_full | Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810 |
| title_fullStr | Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810 |
| title_full_unstemmed | Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810 |
| title_short | Cells and Cytokines in Milk of Subclinically Infected Bovine Mammary Glands after the Use of Immunomodulatory Composition GLP 810 |
| title_sort | cells and cytokines in milk of subclinically infected bovine mammary glands after the use of immunomodulatory composition glp 810 |
| url | http://dx.doi.org/10.1155/2020/8238029 |
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