Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes
Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strate...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2011/756905 |
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| author | Sarah Tuttleton Arron Peter Skewes-Cox Phong H. Do Eric Dybbro Maria Da Costa Joel M. Palefsky Joseph L. DeRisi |
| author_facet | Sarah Tuttleton Arron Peter Skewes-Cox Phong H. Do Eric Dybbro Maria Da Costa Joel M. Palefsky Joseph L. DeRisi |
| author_sort | Sarah Tuttleton Arron |
| collection | DOAJ |
| description | Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values. |
| format | Article |
| id | doaj-art-31dcb4253771452e9f8615797a33c52b |
| institution | Kabale University |
| issn | 2090-021X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-31dcb4253771452e9f8615797a33c52b2025-02-03T00:59:29ZengWileyJournal of Nucleic Acids2090-021X2011-01-01201110.4061/2011/756905756905Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 GenotypesSarah Tuttleton Arron0Peter Skewes-Cox1Phong H. Do2Eric Dybbro3Maria Da Costa4Joel M. Palefsky5Joseph L. DeRisi6Department of Dermatology, University of California San Francisco, San Francisco, CA 94143, USABiological and Medical Informatics Program, University of California San Francisco, San Francisco, CA 94143, USADepartment of Dermatology, University of California San Francisco, San Francisco, CA 94143, USADepartment of Dermatology, University of California San Francisco, San Francisco, CA 94143, USADepartment of Medicine, University of California San Francisco, San Francisco, CA 94143, USADepartment of Medicine, University of California San Francisco, San Francisco, CA 94143, USADepartment of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USAPapillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values.http://dx.doi.org/10.4061/2011/756905 |
| spellingShingle | Sarah Tuttleton Arron Peter Skewes-Cox Phong H. Do Eric Dybbro Maria Da Costa Joel M. Palefsky Joseph L. DeRisi Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes Journal of Nucleic Acids |
| title | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
| title_full | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
| title_fullStr | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
| title_full_unstemmed | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
| title_short | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
| title_sort | validation of a diagnostic microarray for human papillomavirus coverage of 102 genotypes |
| url | http://dx.doi.org/10.4061/2011/756905 |
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