Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combination
The induction effect of excess Ca2+ and Mg2+ alone or in combination on settlement and metamorphosis of Mercenaria mercenaria larvae were studied by initiating ion supplementation at two different developmental stages. Pediveligers were supplemented with a range of excess Ca2+ (0–18 mM), Mg2+ (0–40 ...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006768 |
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| author | Jian Liang Bajun Shi Yongren Li Shuang Liang Huiru Liu Wei Dai Yongjun Guo |
| author_facet | Jian Liang Bajun Shi Yongren Li Shuang Liang Huiru Liu Wei Dai Yongjun Guo |
| author_sort | Jian Liang |
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| description | The induction effect of excess Ca2+ and Mg2+ alone or in combination on settlement and metamorphosis of Mercenaria mercenaria larvae were studied by initiating ion supplementation at two different developmental stages. Pediveligers were supplemented with a range of excess Ca2+ (0–18 mM), Mg2+ (0–40 mM) and Ca2＋/ Mg2+ (0–7.5/0–37.5 mM), respectively. Results showed excess Ca2+ and Mg2+ alone or in combination could induce settlement and metamorphosis of M. mercenaria. The optimal concentrations of Ca2+, Mg2+, and Ca2+/Mg2+ were 10 mM, 20 mM and 3.125/15.625 mM, respectively. The supplementation of the above optimal concentration of ions from D-shaped larvae stage also significantly induced larval metamorphosis (P < 0.05), and at the same concentration, the induction effect was better than that initiating from pediveliger stage. In addition, compared with the control group, ion supplementation from D-shaped larvae stage significantly increased the survival rate of pediveligers (P < 0.05) and prolonged the planktonic duration. The longer the planktonic duration, the larger the larval size at the end of the planktonic stage and the higher metamorphosis rate of larvae. In the hatchery seed production of M. mercenaria, it is recommended to supplement 3.125/15.625 mM Ca2＋/ Mg2+ from the D-shaped larvae stage to induce the settlement and metamorphosis. |
| format | Article |
| id | doaj-art-31d67765fc534d55ab25e67ff03ce268 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-31d67765fc534d55ab25e67ff03ce2682025-02-06T05:12:13ZengElsevierAquaculture Reports2352-51342025-03-0140102588Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combinationJian Liang0Bajun Shi1Yongren Li2Shuang Liang3Huiru Liu4Wei Dai5Yongjun Guo6Fisheries College, Guangdong Ocean University, Zhanjiang, China; Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, China; Key Laboratory of Smart Breeding (Co-construction by Ministry and Province, Ministry of Agriculture and Rural Affairs), Tianjin Agricultural University, Tianjin 300384, ChinaTianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, ChinaTianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, China; Key Laboratory of Smart Breeding (Co-construction by Ministry and Province, Ministry of Agriculture and Rural Affairs), Tianjin Agricultural University, Tianjin 300384, ChinaTianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, China; Key Laboratory of Smart Breeding (Co-construction by Ministry and Province, Ministry of Agriculture and Rural Affairs), Tianjin Agricultural University, Tianjin 300384, ChinaTianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, China; Key Laboratory of Smart Breeding (Co-construction by Ministry and Province, Ministry of Agriculture and Rural Affairs), Tianjin Agricultural University, Tianjin 300384, ChinaTianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, China; Key Laboratory of Smart Breeding (Co-construction by Ministry and Province, Ministry of Agriculture and Rural Affairs), Tianjin Agricultural University, Tianjin 300384, ChinaTianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, China; Key Laboratory of Smart Breeding (Co-construction by Ministry and Province, Ministry of Agriculture and Rural Affairs), Tianjin Agricultural University, Tianjin 300384, China; Corresponding author at: Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, China.The induction effect of excess Ca2+ and Mg2+ alone or in combination on settlement and metamorphosis of Mercenaria mercenaria larvae were studied by initiating ion supplementation at two different developmental stages. Pediveligers were supplemented with a range of excess Ca2+ (0–18 mM), Mg2+ (0–40 mM) and Ca2＋/ Mg2+ (0–7.5/0–37.5 mM), respectively. Results showed excess Ca2+ and Mg2+ alone or in combination could induce settlement and metamorphosis of M. mercenaria. The optimal concentrations of Ca2+, Mg2+, and Ca2+/Mg2+ were 10 mM, 20 mM and 3.125/15.625 mM, respectively. The supplementation of the above optimal concentration of ions from D-shaped larvae stage also significantly induced larval metamorphosis (P < 0.05), and at the same concentration, the induction effect was better than that initiating from pediveliger stage. In addition, compared with the control group, ion supplementation from D-shaped larvae stage significantly increased the survival rate of pediveligers (P < 0.05) and prolonged the planktonic duration. The longer the planktonic duration, the larger the larval size at the end of the planktonic stage and the higher metamorphosis rate of larvae. In the hatchery seed production of M. mercenaria, it is recommended to supplement 3.125/15.625 mM Ca2＋/ Mg2+ from the D-shaped larvae stage to induce the settlement and metamorphosis.http://www.sciencedirect.com/science/article/pii/S2352513424006768IonInduction effectSettlementMetamorphosisM. mercenaria |
| spellingShingle | Jian Liang Bajun Shi Yongren Li Shuang Liang Huiru Liu Wei Dai Yongjun Guo Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combination Aquaculture Reports Ion Induction effect Settlement Metamorphosis M. mercenaria |
| title | Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combination |
| title_full | Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combination |
| title_fullStr | Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combination |
| title_full_unstemmed | Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combination |
| title_short | Induction of larval settlement and metamorphosis of Mercenaria mercenaria using excess Ca2＋and Mg2+alone or in combination |
| title_sort | induction of larval settlement and metamorphosis of mercenaria mercenaria using excess ca2 and mg2 alone or in combination |
| topic | Ion Induction effect Settlement Metamorphosis M. mercenaria |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006768 |
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