HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor Microenvironment
Background/Objectives: Effectively targeting treatment-resistant tumor cells, particularly cancer stem cells (CSCs) involved in tumor recurrence, remains a major challenge in immunotherapy. This study examines the potential of combining mechanical high-intensity focused ultrasound (M-HIFU) with dend...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/17/1/65 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587700376436736 |
|---|---|
| author | Bum-Seo Baek Hyunmi Park Ji-Woong Choi Eun-Young Lee Seung-Yong Seong |
| author_facet | Bum-Seo Baek Hyunmi Park Ji-Woong Choi Eun-Young Lee Seung-Yong Seong |
| author_sort | Bum-Seo Baek |
| collection | DOAJ |
| description | Background/Objectives: Effectively targeting treatment-resistant tumor cells, particularly cancer stem cells (CSCs) involved in tumor recurrence, remains a major challenge in immunotherapy. This study examines the potential of combining mechanical high-intensity focused ultrasound (M-HIFU) with dendritic cell (DC) vaccines to enhance immune responses against OLFM4-expressing tumors, a CSC marker linked to immune evasion and tumor growth. Methods: M-HIFU was applied to induce immunogenic cell death by mechanically disrupting tumor cells, releasing tumor-associated antigens and creating an immunostimulatory environment. DC vaccines loaded with OLFM4 were then administered to boost the immune response within this primed environment. Results: The combination of M-HIFU and DC vaccine significantly inhibited tumor growth and metastasis, with enhanced T-cell activation and increased recruitment of immune cells due to elevated chemokines CCL19 and CCL21. This synergy promoted immune memory, reducing the likelihood of recurrence. Conclusions: M-HIFU effectively promotes the migration of DC vaccines through CCL19/21, presenting a promising approach for cancer treatment. Further studies are recommended to optimize this combination for clinical applications, with potential to improve patient outcomes in challenging cancer types. |
| format | Article |
| id | doaj-art-31cde5553fd746de932c21e09bd18e44 |
| institution | Kabale University |
| issn | 1999-4923 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj-art-31cde5553fd746de932c21e09bd18e442025-01-24T13:45:47ZengMDPI AGPharmaceutics1999-49232025-01-011716510.3390/pharmaceutics17010065HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor MicroenvironmentBum-Seo Baek0Hyunmi Park1Ji-Woong Choi2Eun-Young Lee3Seung-Yong Seong4Wide River Institute of Immunology, Seoul National University College of Medicine, Hongcheon 25159, Gangwon, Republic of KoreaWide River Institute of Immunology, Seoul National University College of Medicine, Hongcheon 25159, Gangwon, Republic of KoreaWide River Institute of Immunology, Seoul National University College of Medicine, Hongcheon 25159, Gangwon, Republic of KoreaWide River Institute of Immunology, Seoul National University College of Medicine, Hongcheon 25159, Gangwon, Republic of KoreaWide River Institute of Immunology, Seoul National University College of Medicine, Hongcheon 25159, Gangwon, Republic of KoreaBackground/Objectives: Effectively targeting treatment-resistant tumor cells, particularly cancer stem cells (CSCs) involved in tumor recurrence, remains a major challenge in immunotherapy. This study examines the potential of combining mechanical high-intensity focused ultrasound (M-HIFU) with dendritic cell (DC) vaccines to enhance immune responses against OLFM4-expressing tumors, a CSC marker linked to immune evasion and tumor growth. Methods: M-HIFU was applied to induce immunogenic cell death by mechanically disrupting tumor cells, releasing tumor-associated antigens and creating an immunostimulatory environment. DC vaccines loaded with OLFM4 were then administered to boost the immune response within this primed environment. Results: The combination of M-HIFU and DC vaccine significantly inhibited tumor growth and metastasis, with enhanced T-cell activation and increased recruitment of immune cells due to elevated chemokines CCL19 and CCL21. This synergy promoted immune memory, reducing the likelihood of recurrence. Conclusions: M-HIFU effectively promotes the migration of DC vaccines through CCL19/21, presenting a promising approach for cancer treatment. Further studies are recommended to optimize this combination for clinical applications, with potential to improve patient outcomes in challenging cancer types.https://www.mdpi.com/1999-4923/17/1/65mechanical high-intensity focused ultrasoundtumor microenvironmentcancer immunotherapydendritic cell vaccines |
| spellingShingle | Bum-Seo Baek Hyunmi Park Ji-Woong Choi Eun-Young Lee Seung-Yong Seong HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor Microenvironment Pharmaceutics mechanical high-intensity focused ultrasound tumor microenvironment cancer immunotherapy dendritic cell vaccines |
| title | HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor Microenvironment |
| title_full | HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor Microenvironment |
| title_fullStr | HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor Microenvironment |
| title_full_unstemmed | HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor Microenvironment |
| title_short | HIFU-CCL19/21 Axis Enhances Dendritic Cell Vaccine Efficacy in the Tumor Microenvironment |
| title_sort | hifu ccl19 21 axis enhances dendritic cell vaccine efficacy in the tumor microenvironment |
| topic | mechanical high-intensity focused ultrasound tumor microenvironment cancer immunotherapy dendritic cell vaccines |
| url | https://www.mdpi.com/1999-4923/17/1/65 |
| work_keys_str_mv | AT bumseobaek hifuccl1921axisenhancesdendriticcellvaccineefficacyinthetumormicroenvironment AT hyunmipark hifuccl1921axisenhancesdendriticcellvaccineefficacyinthetumormicroenvironment AT jiwoongchoi hifuccl1921axisenhancesdendriticcellvaccineefficacyinthetumormicroenvironment AT eunyounglee hifuccl1921axisenhancesdendriticcellvaccineefficacyinthetumormicroenvironment AT seungyongseong hifuccl1921axisenhancesdendriticcellvaccineefficacyinthetumormicroenvironment |