Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibition
In the face of increasingly serious environmental pollution, the health of human lung tissues is also facing serious threats. Mogroside ⅡE (M2E) is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori. The study elucidated the anti-inflammatory...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2024-01-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023001362 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832553386506977280 |
|---|---|
| author | Weichao Lü Guoqing Ren Kuniyoshi Shimizu Renshi Li Chaofeng Zhang |
| author_facet | Weichao Lü Guoqing Ren Kuniyoshi Shimizu Renshi Li Chaofeng Zhang |
| author_sort | Weichao Lü |
| collection | DOAJ |
| description | In the face of increasingly serious environmental pollution, the health of human lung tissues is also facing serious threats. Mogroside ⅡE (M2E) is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori. The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury (ALI). A lipopolysaccharide (LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting, co-immunoprecipitation, and quantitative real time-PCR analysis. The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA (Pla2g2a)-epidermal growth factor receptor (EGFR). The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation. In addition, M2E protected ALI induced with LPS against inflammatory and damage which were significantly dependent upon the downregulation of AKT and mTOR via the inhibition of Pla2g2a-EGFR. Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury, which may represent a promising strategy to treat ALI. |
| format | Article |
| id | doaj-art-31c700b7339944aa904a14e88453128e |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-31c700b7339944aa904a14e88453128e2025-02-03T05:54:06ZengTsinghua University PressFood Science and Human Wellness2213-45302024-01-01131299312Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibitionWeichao Lü0Guoqing Ren1Kuniyoshi Shimizu2Renshi Li3Chaofeng Zhang4State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaState Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, ChinaSino-Jan Joint Lab of Natural Health Products Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Faculty of Agriculture, Kyushu University, Fukuoka 819-0395, JapanState Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Sino-Jan Joint Lab of Natural Health Products Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Corresponding author at: School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Sino-Jan Joint Lab of Natural Health Products Research, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China; Corresponding author at: School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China.In the face of increasingly serious environmental pollution, the health of human lung tissues is also facing serious threats. Mogroside ⅡE (M2E) is the main metabolite of sweetening agents mogrosides from the anti-tussive Chinese herbal Siraitia grosvenori. The study elucidated the anti-inflammatory action and molecular mechanism of M2E against acute lung injury (ALI). A lipopolysaccharide (LPS)-induced ALI model was established in mice and MH-S cells were employed to explore the protective mechanism of M2E through the western blotting, co-immunoprecipitation, and quantitative real time-PCR analysis. The results indicated that M2E alleviated LPS-induced lung injury through restraining the activation of secreted phospholipase A2 type IIA (Pla2g2a)-epidermal growth factor receptor (EGFR). The interaction of Pla2g2a and EGFR was identified by co-immunoprecipitation. In addition, M2E protected ALI induced with LPS against inflammatory and damage which were significantly dependent upon the downregulation of AKT and mTOR via the inhibition of Pla2g2a-EGFR. Pla2g2a may represent a potential target for M2E in the improvement of LPS-induced lung injury, which may represent a promising strategy to treat ALI.http://www.sciencedirect.com/science/article/pii/S2213453023001362Mogroside ⅡEAcute lung injurySecreted phospholipase A2 type IIA (Pla2g2a)Epidermal growth factor receptor (EGFR) |
| spellingShingle | Weichao Lü Guoqing Ren Kuniyoshi Shimizu Renshi Li Chaofeng Zhang Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibition Food Science and Human Wellness Mogroside ⅡE Acute lung injury Secreted phospholipase A2 type IIA (Pla2g2a) Epidermal growth factor receptor (EGFR) |
| title | Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibition |
| title_full | Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibition |
| title_fullStr | Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibition |
| title_full_unstemmed | Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibition |
| title_short | Mogroside ⅡE, an in vivo metabolite of sweet agent, alleviates acute lung injury via Pla2g2a-EGFR inhibition |
| title_sort | mogroside iie an in vivo metabolite of sweet agent alleviates acute lung injury via pla2g2a egfr inhibition |
| topic | Mogroside ⅡE Acute lung injury Secreted phospholipase A2 type IIA (Pla2g2a) Epidermal growth factor receptor (EGFR) |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023001362 |
| work_keys_str_mv | AT weichaolu mogrosideiieaninvivometaboliteofsweetagentalleviatesacutelunginjuryviapla2g2aegfrinhibition AT guoqingren mogrosideiieaninvivometaboliteofsweetagentalleviatesacutelunginjuryviapla2g2aegfrinhibition AT kuniyoshishimizu mogrosideiieaninvivometaboliteofsweetagentalleviatesacutelunginjuryviapla2g2aegfrinhibition AT renshili mogrosideiieaninvivometaboliteofsweetagentalleviatesacutelunginjuryviapla2g2aegfrinhibition AT chaofengzhang mogrosideiieaninvivometaboliteofsweetagentalleviatesacutelunginjuryviapla2g2aegfrinhibition |