Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of Usvyaty
Background. The data are presented on the architectonics of ancient barley spikes from the 12th century, excavated in 2019 at Usvyaty Settlement. Modern molecular genetics approaches were used to study domestication genes (Btr1, Btr2, and Vrs) in ancient and contemporary barleys (germplasm accession...
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| Format: | Article |
| Language: | English |
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N.I. Vavilov All-Russian Institute of Plant Genetic Resources
2024-10-01
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| Series: | Труды по прикладной ботанике, генетике и селекции |
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| Online Access: | https://elpub.vir.nw.ru/jour/article/view/2041 |
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| author | T. V. Semilet N. V. Smirnova N. A. Shvachko O. N. Kovaleva E. K. Khlestkina |
| author_facet | T. V. Semilet N. V. Smirnova N. A. Shvachko O. N. Kovaleva E. K. Khlestkina |
| author_sort | T. V. Semilet |
| collection | DOAJ |
| description | Background. The data are presented on the architectonics of ancient barley spikes from the 12th century, excavated in 2019 at Usvyaty Settlement. Modern molecular genetics approaches were used to study domestication genes (Btr1, Btr2, and Vrs) in ancient and contemporary barleys (germplasm accessions preserved at VIR).Materials and methods. The carbonized kernels found by archaeologists during the excavations at Usvyaty were analyzed. Primers for domestication genes were designed, and PCR was performed on contemporary and ancient barley grains. Ancient kernels were studied in accordance with the rules established for organizing a paleogenetics laboratory, which excluded any contamination with contemporary DNA. Fragments of domestication genes from contemporary and ancient barley grain samples underwent Sanger sequencing. Results. Ancient DNA was isolated and enriched. The analysis of domestication gene sequences made it possible to reconstruct the ancient barley spike’s features. Conclusion. The ancient cereal crop architectonics was restored to ascertain a brittle two-row spike of ancient barley |
| format | Article |
| id | doaj-art-31be2b63c0924b63b1f5bfca8d7cf4a9 |
| institution | Kabale University |
| issn | 2227-8834 2619-0982 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | N.I. Vavilov All-Russian Institute of Plant Genetic Resources |
| record_format | Article |
| series | Труды по прикладной ботанике, генетике и селекции |
| spelling | doaj-art-31be2b63c0924b63b1f5bfca8d7cf4a92025-02-03T08:31:17ZengN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesТруды по прикладной ботанике, генетике и селекции2227-88342619-09822024-10-01185319920910.30901/2227-8834-2024-3-199-209827Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of UsvyatyT. V. Semilet0N. V. Smirnova1N. A. Shvachko2O. N. Kovaleva3E. K. Khlestkina4N.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic ResourcesN.I. Vavilov All-Russian Institute of Plant Genetic Resources; Sirius University of Science and Technology, Research Center of Genetics and Life SciencesBackground. The data are presented on the architectonics of ancient barley spikes from the 12th century, excavated in 2019 at Usvyaty Settlement. Modern molecular genetics approaches were used to study domestication genes (Btr1, Btr2, and Vrs) in ancient and contemporary barleys (germplasm accessions preserved at VIR).Materials and methods. The carbonized kernels found by archaeologists during the excavations at Usvyaty were analyzed. Primers for domestication genes were designed, and PCR was performed on contemporary and ancient barley grains. Ancient kernels were studied in accordance with the rules established for organizing a paleogenetics laboratory, which excluded any contamination with contemporary DNA. Fragments of domestication genes from contemporary and ancient barley grain samples underwent Sanger sequencing. Results. Ancient DNA was isolated and enriched. The analysis of domestication gene sequences made it possible to reconstruct the ancient barley spike’s features. Conclusion. The ancient cereal crop architectonics was restored to ascertain a brittle two-row spike of ancient barleyhttps://elpub.vir.nw.ru/jour/article/view/2041ancient dna<i>btr1</i><i>btr2</i><i>vrs</i><i>hordeum vulgare</i> l. |
| spellingShingle | T. V. Semilet N. V. Smirnova N. A. Shvachko O. N. Kovaleva E. K. Khlestkina Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of Usvyaty Труды по прикладной ботанике, генетике и селекции ancient dna <i>btr1</i> <i>btr2</i> <i>vrs</i> <i>hordeum vulgare</i> l. |
| title | Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of Usvyaty |
| title_full | Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of Usvyaty |
| title_fullStr | Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of Usvyaty |
| title_full_unstemmed | Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of Usvyaty |
| title_short | Restoration of the spike architectonics in ancient barley excavated at the twelfth-century settlement of Usvyaty |
| title_sort | restoration of the spike architectonics in ancient barley excavated at the twelfth century settlement of usvyaty |
| topic | ancient dna <i>btr1</i> <i>btr2</i> <i>vrs</i> <i>hordeum vulgare</i> l. |
| url | https://elpub.vir.nw.ru/jour/article/view/2041 |
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