Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages
Macrophages are important cells of the innate immunity that play a major role in Bovine Viral Diarrhoea Virus (BVDV) pathogenesis. Macrophages are not a homogenous population; they exist in different phenotypes, typically divided into two main categories: classically (pro-inflammatory) and alternati...
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Taylor & Francis Group
2024-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2023.2283899 |
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| author | Lucas José Barone Nancy Patricia Cardoso Florencia Celeste Mansilla Mariángeles Castillo Alejandra Victoria Capozzo |
| author_facet | Lucas José Barone Nancy Patricia Cardoso Florencia Celeste Mansilla Mariángeles Castillo Alejandra Victoria Capozzo |
| author_sort | Lucas José Barone |
| collection | DOAJ |
| description | Macrophages are important cells of the innate immunity that play a major role in Bovine Viral Diarrhoea Virus (BVDV) pathogenesis. Macrophages are not a homogenous population; they exist in different phenotypes, typically divided into two main categories: classically (pro-inflammatory) and alternatively activated (anti-inflammatory) or M1 and M2, respectively. The role of bovine macrophage phenotypes on BVDV infection is still unclear. This study characterized the interaction between BVDV and monocyte-derived macrophages (Mo-Mφ) collected from healthy cattle and polarized to an M1 or M2 state by using LPS, INF-γ, IL-4, or azithromycin. Arginase activity quantitation was utilized as a marker of the M2 Mo-Mφ spectrum. There was a significant association between arginase activity and the replication rate of BVDV strains of different genotypes and biotypes. Inhibition of arginase activity also reduced BVDV infectivity. Calves treated with azithromycin-induced Mo-Mφ of the M2 state produced high levels of arginase. Interestingly, azithromycin administered in vivo increased the susceptibility of macrophages to BVDV infection ex vivo. Mo-Mφ from pregnant dams and calves produced higher arginase levels than those from non-pregnant adult animals. The increased infection of arginase-producing alternatively activated bovine macrophages with BVDV supports the need to delve into a possible leading role of M2 macrophages in establishing the immune-suppressive state during BVDV convalescence. |
| format | Article |
| id | doaj-art-31b78bc90d244fa18102f5c48013b9f2 |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Virulence |
| spelling | doaj-art-31b78bc90d244fa18102f5c48013b9f22025-01-23T03:42:51ZengTaylor & Francis GroupVirulence2150-55942150-56082024-12-0115110.1080/21505594.2023.2283899Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophagesLucas José Barone0Nancy Patricia Cardoso1Florencia Celeste Mansilla2Mariángeles Castillo3Alejandra Victoria Capozzo4Institute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, ArgentinaInstitute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, ArgentinaInstitute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, ArgentinaInstitute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, ArgentinaInstitute of Virology and Technical Innovations, INTA- CONICET. National Research Council (CONICET), Buenos Aires, ArgentinaMacrophages are important cells of the innate immunity that play a major role in Bovine Viral Diarrhoea Virus (BVDV) pathogenesis. Macrophages are not a homogenous population; they exist in different phenotypes, typically divided into two main categories: classically (pro-inflammatory) and alternatively activated (anti-inflammatory) or M1 and M2, respectively. The role of bovine macrophage phenotypes on BVDV infection is still unclear. This study characterized the interaction between BVDV and monocyte-derived macrophages (Mo-Mφ) collected from healthy cattle and polarized to an M1 or M2 state by using LPS, INF-γ, IL-4, or azithromycin. Arginase activity quantitation was utilized as a marker of the M2 Mo-Mφ spectrum. There was a significant association between arginase activity and the replication rate of BVDV strains of different genotypes and biotypes. Inhibition of arginase activity also reduced BVDV infectivity. Calves treated with azithromycin-induced Mo-Mφ of the M2 state produced high levels of arginase. Interestingly, azithromycin administered in vivo increased the susceptibility of macrophages to BVDV infection ex vivo. Mo-Mφ from pregnant dams and calves produced higher arginase levels than those from non-pregnant adult animals. The increased infection of arginase-producing alternatively activated bovine macrophages with BVDV supports the need to delve into a possible leading role of M2 macrophages in establishing the immune-suppressive state during BVDV convalescence.https://www.tandfonline.com/doi/10.1080/21505594.2023.2283899Bovine macrophagesarginase activitypestivirusazithromycin immunosuppressionreplication |
| spellingShingle | Lucas José Barone Nancy Patricia Cardoso Florencia Celeste Mansilla Mariángeles Castillo Alejandra Victoria Capozzo Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages Virulence Bovine macrophages arginase activity pestivirus azithromycin immunosuppression replication |
| title | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_full | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_fullStr | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_full_unstemmed | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_short | Enhanced infectivity of bovine viral diarrhoea virus (BVDV) in arginase-producing bovine monocyte-derived macrophages |
| title_sort | enhanced infectivity of bovine viral diarrhoea virus bvdv in arginase producing bovine monocyte derived macrophages |
| topic | Bovine macrophages arginase activity pestivirus azithromycin immunosuppression replication |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2023.2283899 |
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