Exploring the Effects of <i>Gracilaria lemaneiformis</i> Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome Sequencing
Sixty Duroc × (Landrace × Yorkshire) castrated male finishing pigs were randomly divided into negative control (NC) and <i>gracilaria lemaneiformis</i> polysaccharides (GLP) groups to study the effects of GLP on the fecal microbiota and fecal metabolites of fattening pigs. The NC group w...
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2025-01-01
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| author | Mingyang Jia Qiang Ma Hongjun Wang Xiangzhou Yan Lei Wang Baosong Xing Qingxia Lu Jing Wang |
| author_facet | Mingyang Jia Qiang Ma Hongjun Wang Xiangzhou Yan Lei Wang Baosong Xing Qingxia Lu Jing Wang |
| author_sort | Mingyang Jia |
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| description | Sixty Duroc × (Landrace × Yorkshire) castrated male finishing pigs were randomly divided into negative control (NC) and <i>gracilaria lemaneiformis</i> polysaccharides (GLP) groups to study the effects of GLP on the fecal microbiota and fecal metabolites of fattening pigs. The NC group was fed a basic diet, and the GLP group was fed a basic diet supplemented with 0.1% GLP. The diversity of the species and the fecal metabolites were analyzed using 16S rDNA sequencing and metabolome sequencing, respectively. The serum immune and antioxidant indices were analyzed using the ELISA assay. The abundances of hazardous bacteria such as Proteobacteria at the phylum level and <i>Shigella</i> at the genus level were extremely significantly decreased (<i>p</i> < 0.01) and those of beneficial bacteria such as Firmicutes at the phylum level (<i>p</i> < 0.01), <i>Clostridium</i> at the genus level (<i>p</i> < 0.01), and <i>Lactobacillus</i> at the genus level (<i>p</i> < 0.05) were significantly increased in the GLP group compared with the NC group. A total of 41 differentially expressed metabolites were identified. The expression of anti-inflammatory and antioxidant active substances, such as methyl cinnamate, protopanaxatriol, and isovanillic acid, was elevated in the GLP group. The ELISA assay showed increased GSH-Px activity (<i>p</i> < 0.01), T-AOC (<i>p</i> < 0.01), IgG (<i>p</i> < 0.01), IgA (<i>p</i> < 0.05), and IgM (<i>p</i> < 0.05) in the GLP group. These results indicate that dietary GLP supplementation can improve the antioxidant ability, anti-inflammatory ability, and immune level of fattening pigs by regulating fecal flora and metabolites and could be used as a functional feed additive. |
| format | Article |
| id | doaj-art-319f1a7627514143b452f3a93dc732b1 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-319f1a7627514143b452f3a93dc732b12025-01-24T13:17:47ZengMDPI AGAnimals2076-26152025-01-0115215310.3390/ani15020153Exploring the Effects of <i>Gracilaria lemaneiformis</i> Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome SequencingMingyang Jia0Qiang Ma1Hongjun Wang2Xiangzhou Yan3Lei Wang4Baosong Xing5Qingxia Lu6Jing Wang7Key Laboratory of Livestock and Poultry Breeding and Nutrition Regulation in Henan Province, Institute of Animal Husbandry, Henanmn Academy of Agricultural Sciences, Zhengzhou 450002, ChinaKey Laboratory of Livestock and Poultry Breeding and Nutrition Regulation in Henan Province, Institute of Animal Husbandry, Henanmn Academy of Agricultural Sciences, Zhengzhou 450002, ChinaAnimal Disease Prevention and Control Center of Xin’an County, Luoyang 471800, ChinaKey Laboratory of Livestock and Poultry Breeding and Nutrition Regulation in Henan Province, Institute of Animal Husbandry, Henanmn Academy of Agricultural Sciences, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Institute of Science and Technology, Xinxiang453003, ChinaKey Laboratory of Livestock and Poultry Breeding and Nutrition Regulation in Henan Province, Institute of Animal Husbandry, Henanmn Academy of Agricultural Sciences, Zhengzhou 450002, ChinaKey Laboratory of Livestock and Poultry Breeding and Nutrition Regulation in Henan Province, Institute of Animal Husbandry, Henanmn Academy of Agricultural Sciences, Zhengzhou 450002, ChinaKey Laboratory of Livestock and Poultry Breeding and Nutrition Regulation in Henan Province, Institute of Animal Husbandry, Henanmn Academy of Agricultural Sciences, Zhengzhou 450002, ChinaSixty Duroc × (Landrace × Yorkshire) castrated male finishing pigs were randomly divided into negative control (NC) and <i>gracilaria lemaneiformis</i> polysaccharides (GLP) groups to study the effects of GLP on the fecal microbiota and fecal metabolites of fattening pigs. The NC group was fed a basic diet, and the GLP group was fed a basic diet supplemented with 0.1% GLP. The diversity of the species and the fecal metabolites were analyzed using 16S rDNA sequencing and metabolome sequencing, respectively. The serum immune and antioxidant indices were analyzed using the ELISA assay. The abundances of hazardous bacteria such as Proteobacteria at the phylum level and <i>Shigella</i> at the genus level were extremely significantly decreased (<i>p</i> < 0.01) and those of beneficial bacteria such as Firmicutes at the phylum level (<i>p</i> < 0.01), <i>Clostridium</i> at the genus level (<i>p</i> < 0.01), and <i>Lactobacillus</i> at the genus level (<i>p</i> < 0.05) were significantly increased in the GLP group compared with the NC group. A total of 41 differentially expressed metabolites were identified. The expression of anti-inflammatory and antioxidant active substances, such as methyl cinnamate, protopanaxatriol, and isovanillic acid, was elevated in the GLP group. The ELISA assay showed increased GSH-Px activity (<i>p</i> < 0.01), T-AOC (<i>p</i> < 0.01), IgG (<i>p</i> < 0.01), IgA (<i>p</i> < 0.05), and IgM (<i>p</i> < 0.05) in the GLP group. These results indicate that dietary GLP supplementation can improve the antioxidant ability, anti-inflammatory ability, and immune level of fattening pigs by regulating fecal flora and metabolites and could be used as a functional feed additive.https://www.mdpi.com/2076-2615/15/2/153fattening pigs<i>gracilaria lemaneiformis</i> polysaccharides16S rDNA sequencinguntargeted metabolomics |
| spellingShingle | Mingyang Jia Qiang Ma Hongjun Wang Xiangzhou Yan Lei Wang Baosong Xing Qingxia Lu Jing Wang Exploring the Effects of <i>Gracilaria lemaneiformis</i> Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome Sequencing Animals fattening pigs <i>gracilaria lemaneiformis</i> polysaccharides 16S rDNA sequencing untargeted metabolomics |
| title | Exploring the Effects of <i>Gracilaria lemaneiformis</i> Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome Sequencing |
| title_full | Exploring the Effects of <i>Gracilaria lemaneiformis</i> Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome Sequencing |
| title_fullStr | Exploring the Effects of <i>Gracilaria lemaneiformis</i> Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome Sequencing |
| title_full_unstemmed | Exploring the Effects of <i>Gracilaria lemaneiformis</i> Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome Sequencing |
| title_short | Exploring the Effects of <i>Gracilaria lemaneiformis</i> Polysaccharides on the Fecal Microbiota and Fecal Metabolites of Fattening Pigs Based on 16S rDNA and Metabolome Sequencing |
| title_sort | exploring the effects of i gracilaria lemaneiformis i polysaccharides on the fecal microbiota and fecal metabolites of fattening pigs based on 16s rdna and metabolome sequencing |
| topic | fattening pigs <i>gracilaria lemaneiformis</i> polysaccharides 16S rDNA sequencing untargeted metabolomics |
| url | https://www.mdpi.com/2076-2615/15/2/153 |
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