The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs
Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from 111 dogs, which were a...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/587024 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551449355091968 |
|---|---|
| author | Oya Bulut Orhan Yapici Oguzhan Avci Atilla Simsek Kamil Atli Irmak Dik Sibel Yavru Sibel Hasircioglu Mehmet Kale Nuri Mamak |
| author_facet | Oya Bulut Orhan Yapici Oguzhan Avci Atilla Simsek Kamil Atli Irmak Dik Sibel Yavru Sibel Hasircioglu Mehmet Kale Nuri Mamak |
| author_sort | Oya Bulut |
| collection | DOAJ |
| description | Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from 111 dogs, which were admitted to the Internal Medicine Clinic of Selcuk University, Faculty of Veterinary Medicine, with clinical symptoms. Seventy-seven dogs were sampled from Isparta and Burdur dog shelters by random sampling, regardless of the clinical findings. Dogs showed a systemic disease, characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Two dogs had corneal opacity and photophobia. In serological studies, 188 serum samples were investigated on the presence of CAV antibodies by ELISA. Total 103 (103/188–54.7%) blood samples were detected to be positive for CAV antibodies by ELISA. However, 85 (85/188–45.2%) blood samples were negative. Blood leukocyte samples from dogs were processed and inoculated onto confluent monolayers of MDCK cells using standard virological techniques. After third passage, cells were examined by direct immunoflourescence test for virus isolation. But positive result was not detected. In conclusion, this study clearly demonstrates the high prevalence of CAV infection in dogs. |
| format | Article |
| id | doaj-art-317da4a9f7c344b1b43988009c3d5beb |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-317da4a9f7c344b1b43988009c3d5beb2025-02-03T06:01:20ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/587024587024The Serological and Virological Investigation of Canine Adenovirus Infection on the DogsOya Bulut0Orhan Yapici1Oguzhan Avci2Atilla Simsek3Kamil Atli4Irmak Dik5Sibel Yavru6Sibel Hasircioglu7Mehmet Kale8Nuri Mamak9Department of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, TurkeyDepartment of Virology, Faculty of Veterinary Medicine, University of Kyrgyzstan-Turkey Manas, Bishkek 720044, KyrgyzstanDepartment of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, TurkeyDepartment of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, TurkeyDepartment of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, TurkeyDepartment of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, TurkeyDepartment of Virology, Faculty of Veterinary Medicine, University of Selcuk, 42075 Konya, TurkeyDepartment of Virology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, 15100 Burdur, TurkeyDepartment of Virology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, 15100 Burdur, TurkeyDepartment of Internal Medicine, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, 15100 Burdur, TurkeyTwo types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from 111 dogs, which were admitted to the Internal Medicine Clinic of Selcuk University, Faculty of Veterinary Medicine, with clinical symptoms. Seventy-seven dogs were sampled from Isparta and Burdur dog shelters by random sampling, regardless of the clinical findings. Dogs showed a systemic disease, characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Two dogs had corneal opacity and photophobia. In serological studies, 188 serum samples were investigated on the presence of CAV antibodies by ELISA. Total 103 (103/188–54.7%) blood samples were detected to be positive for CAV antibodies by ELISA. However, 85 (85/188–45.2%) blood samples were negative. Blood leukocyte samples from dogs were processed and inoculated onto confluent monolayers of MDCK cells using standard virological techniques. After third passage, cells were examined by direct immunoflourescence test for virus isolation. But positive result was not detected. In conclusion, this study clearly demonstrates the high prevalence of CAV infection in dogs.http://dx.doi.org/10.1155/2013/587024 |
| spellingShingle | Oya Bulut Orhan Yapici Oguzhan Avci Atilla Simsek Kamil Atli Irmak Dik Sibel Yavru Sibel Hasircioglu Mehmet Kale Nuri Mamak The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs The Scientific World Journal |
| title | The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs |
| title_full | The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs |
| title_fullStr | The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs |
| title_full_unstemmed | The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs |
| title_short | The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs |
| title_sort | serological and virological investigation of canine adenovirus infection on the dogs |
| url | http://dx.doi.org/10.1155/2013/587024 |
| work_keys_str_mv | AT oyabulut theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT orhanyapici theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT oguzhanavci theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT atillasimsek theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT kamilatli theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT irmakdik theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT sibelyavru theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT sibelhasircioglu theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT mehmetkale theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT nurimamak theserologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT oyabulut serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT orhanyapici serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT oguzhanavci serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT atillasimsek serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT kamilatli serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT irmakdik serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT sibelyavru serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT sibelhasircioglu serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT mehmetkale serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs AT nurimamak serologicalandvirologicalinvestigationofcanineadenovirusinfectiononthedogs |