Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells
The role of the L-arginine–nitric oxide metabolic pathway was explored for interleukin-2-induced proliferation in the cytotoxic T lymphocyte clone CTLL-2. Specific inhibition of nitric oxide synthase significantly diminished, in a concentration-dependent manner, 3H-thymidine uptake of CTLL-2 cells i...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1996-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935196000464 |
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| _version_ | 1832568278016327680 |
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| author | J. Padrón L. Glaría O. Martinez M. Torres E. Lopez R. Delgado L. Caveda A. Rojas |
| author_facet | J. Padrón L. Glaría O. Martinez M. Torres E. Lopez R. Delgado L. Caveda A. Rojas |
| author_sort | J. Padrón |
| collection | DOAJ |
| description | The role of the L-arginine–nitric oxide metabolic pathway was explored for interleukin-2-induced proliferation in the cytotoxic T lymphocyte clone CTLL-2. Specific inhibition of nitric oxide synthase significantly diminished, in a concentration-dependent manner, 3H-thymidine uptake of CTLL-2 cells in response to different concentrations of interleukin 2. Withdrawal of L-arginine from culture medium resulted as potent as the higher inhibition obtained when blocking nitric oxide synthase with L-arginine analogues. Furthermore, intermedial concentrations of Larginine and exogenous nitric oxide donors were found for achieving optimal IL2-induced proliferation of CTLL-2. These findings prompted us to suggest that intra- and/or inter-cellular nitric oxide signalling may contribute to the modulation of the IL2 mitogenic effect upon cytotoxic T lymphocytes. |
| format | Article |
| id | doaj-art-31798757423a4dea85825c45ca88d151 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-31798757423a4dea85825c45ca88d1512025-02-03T00:59:21ZengWileyMediators of Inflammation0962-93511466-18611996-01-015532432710.1155/S0962935196000464Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cellsJ. Padrón0L. Glaría1O. Martinez2M. Torres3E. Lopez4R. Delgado5L. Caveda6A. Rojas7Finlay Institute, Department of Basic and Clinical Immunology, P.O. Box 16017, Havana, CubaCenter of Pharmaceutical Chemistry, Department of Pharmacology and Toxicology, P.O. Box 6990, Havana, CubaCenter of Pharmaceutical Chemistry, Department of Pharmacology and Toxicology, P.O. Box 6990, Havana, CubaCenter of Pharmaceutical Chemistry, Department of Pharmacology and Toxicology, P.O. Box 6990, Havana, CubaCenter of Pharmaceutical Chemistry, Department of Pharmacology and Toxicology, P.O. Box 6990, Havana, CubaCenter of Pharmaceutical Chemistry, Department of Pharmacology and Toxicology, P.O. Box 6990, Havana, CubaCenter of Pharmaceutical Chemistry, Department of Pharmacology and Toxicology, P.O. Box 6990, Havana, CubaCenter of Pharmaceutical Chemistry, Department of Pharmacology and Toxicology, P.O. Box 6990, Havana, CubaThe role of the L-arginine–nitric oxide metabolic pathway was explored for interleukin-2-induced proliferation in the cytotoxic T lymphocyte clone CTLL-2. Specific inhibition of nitric oxide synthase significantly diminished, in a concentration-dependent manner, 3H-thymidine uptake of CTLL-2 cells in response to different concentrations of interleukin 2. Withdrawal of L-arginine from culture medium resulted as potent as the higher inhibition obtained when blocking nitric oxide synthase with L-arginine analogues. Furthermore, intermedial concentrations of Larginine and exogenous nitric oxide donors were found for achieving optimal IL2-induced proliferation of CTLL-2. These findings prompted us to suggest that intra- and/or inter-cellular nitric oxide signalling may contribute to the modulation of the IL2 mitogenic effect upon cytotoxic T lymphocytes.http://dx.doi.org/10.1155/S0962935196000464 |
| spellingShingle | J. Padrón L. Glaría O. Martinez M. Torres E. Lopez R. Delgado L. Caveda A. Rojas Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells Mediators of Inflammation |
| title | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
| title_full | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
| title_fullStr | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
| title_full_unstemmed | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
| title_short | Nitric oxide modulates interleukin-2-induced proliferation in CTLL-2 cells |
| title_sort | nitric oxide modulates interleukin 2 induced proliferation in ctll 2 cells |
| url | http://dx.doi.org/10.1155/S0962935196000464 |
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