Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis
Aims. Nutrient deficiencies are common in patients with inflammatory bowel disease (IBD). Adipose tissue plays a critical role in regulating energy balance. Fibroblast growth factor 21 (FGF21) is an important endocrine metabolic regulator with emerging beneficial roles in lipid homeostasis. We inves...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/3089378 |
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| author | Liming Liu Cuiqing Zhao Ying Yang Xiaoxia Kong Tuo Shao Li Ren Xinyu Zhuang Baishuang Yin Gerald Dryden Craig McClain Weimin Luan Wenke Feng |
| author_facet | Liming Liu Cuiqing Zhao Ying Yang Xiaoxia Kong Tuo Shao Li Ren Xinyu Zhuang Baishuang Yin Gerald Dryden Craig McClain Weimin Luan Wenke Feng |
| author_sort | Liming Liu |
| collection | DOAJ |
| description | Aims. Nutrient deficiencies are common in patients with inflammatory bowel disease (IBD). Adipose tissue plays a critical role in regulating energy balance. Fibroblast growth factor 21 (FGF21) is an important endocrine metabolic regulator with emerging beneficial roles in lipid homeostasis. We investigated the impact of FGF21 in experimental colitis-induced epididymal white adipose tissue (eWAT) lipolysis. Methods. Mice were given 2.5% dextran sulfate sodium (DSS) ad libitum for 7 days to induce colitis. The role of FGF21 was investigated using antibody neutralization or knockout (KO) mice. Lipolysis index and adipose lipolytic enzymes were determined. In addition, 3T3-L1 cells were pretreated with IL-6, followed by recombinant human FGF21 (rhFGF21) treatment; lipolysis was assessed. Results. DSS markedly decreased eWAT/body weight ratio and increased serum concentrations of free fatty acid (FFA) and glycerol, indicating increased adipose tissue lipolysis. eWAT intracellular lipolytic enzyme expression/activation was significantly increased. These alterations were significantly attenuated in FGF21 KO mice and by circulating FGF21 neutralization. Moreover, DSS treatment markedly increased serum IL-6 and FGF21 levels. IL-6 pretreatment was necessary for the stimulatory effect of FGF21 on adipose lipolysis in 3T3-L1 cells. Conclusions. Our results demonstrate that experimental colitis induces eWAT lipolysis via an IL-6/FGF21-mediated signaling pathway. |
| format | Article |
| id | doaj-art-3144b2395f6d4048acaf524e13e70712 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-3144b2395f6d4048acaf524e13e707122025-02-03T01:09:10ZengWileyGastroenterology Research and Practice1687-61211687-630X2017-01-01201710.1155/2017/30893783089378Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue LipolysisLiming Liu0Cuiqing Zhao1Ying Yang2Xiaoxia Kong3Tuo Shao4Li Ren5Xinyu Zhuang6Baishuang Yin7Gerald Dryden8Craig McClain9Weimin Luan10Wenke Feng11College of Animal Science and Technology, Key Laboratory of Animal Production and Production Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaDepartments of Medicine, Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USASchools of Pharmacy and Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325027, ChinaSchools of Pharmacy and Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325027, ChinaDepartments of Medicine, Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USAFirst Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi 710061, ChinaInstitute of Military Veterinary Institute, Academy of Military Medical Science, Changchun, Jilin 130122, ChinaCollege of Animal Science and Technology, Key Lab of Preventive Veterinary Medicine in Jilin Province, Jilin Agricultural Science and Technology University, Jilin, Jilin 132101, ChinaDepartments of Medicine, Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USADepartments of Medicine, Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USACollege of Animal Science and Technology, Key Laboratory of Animal Production and Production Quality and Security, Ministry of Education, Jilin Agricultural University, Changchun, Jilin 130118, ChinaDepartments of Medicine, Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, USAAims. Nutrient deficiencies are common in patients with inflammatory bowel disease (IBD). Adipose tissue plays a critical role in regulating energy balance. Fibroblast growth factor 21 (FGF21) is an important endocrine metabolic regulator with emerging beneficial roles in lipid homeostasis. We investigated the impact of FGF21 in experimental colitis-induced epididymal white adipose tissue (eWAT) lipolysis. Methods. Mice were given 2.5% dextran sulfate sodium (DSS) ad libitum for 7 days to induce colitis. The role of FGF21 was investigated using antibody neutralization or knockout (KO) mice. Lipolysis index and adipose lipolytic enzymes were determined. In addition, 3T3-L1 cells were pretreated with IL-6, followed by recombinant human FGF21 (rhFGF21) treatment; lipolysis was assessed. Results. DSS markedly decreased eWAT/body weight ratio and increased serum concentrations of free fatty acid (FFA) and glycerol, indicating increased adipose tissue lipolysis. eWAT intracellular lipolytic enzyme expression/activation was significantly increased. These alterations were significantly attenuated in FGF21 KO mice and by circulating FGF21 neutralization. Moreover, DSS treatment markedly increased serum IL-6 and FGF21 levels. IL-6 pretreatment was necessary for the stimulatory effect of FGF21 on adipose lipolysis in 3T3-L1 cells. Conclusions. Our results demonstrate that experimental colitis induces eWAT lipolysis via an IL-6/FGF21-mediated signaling pathway.http://dx.doi.org/10.1155/2017/3089378 |
| spellingShingle | Liming Liu Cuiqing Zhao Ying Yang Xiaoxia Kong Tuo Shao Li Ren Xinyu Zhuang Baishuang Yin Gerald Dryden Craig McClain Weimin Luan Wenke Feng Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis Gastroenterology Research and Practice |
| title | Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis |
| title_full | Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis |
| title_fullStr | Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis |
| title_full_unstemmed | Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis |
| title_short | Fibroblast Growth Factor 21 Deficiency Attenuates Experimental Colitis-Induced Adipose Tissue Lipolysis |
| title_sort | fibroblast growth factor 21 deficiency attenuates experimental colitis induced adipose tissue lipolysis |
| url | http://dx.doi.org/10.1155/2017/3089378 |
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