MicroRNA-150-3p enhances the antitumour effects of CGP57380 and is associated with a favourable prognosis in non-small cell lung cancer
Abstract MicroRNA (miRNA) dysregulation has been identified in several carcinomas, including non-small cell lung cancer (NSCLC), and is known to play a role in the development and progression of this disease. We initially conducted a miRNA microarray analysis, which revealed that the MNK inhibitor C...
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2025-01-01
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| author | Hongmei Zheng Songqing Fan Jiadi Luo Qiuyuan Wen Hongjing Zang |
| author_facet | Hongmei Zheng Songqing Fan Jiadi Luo Qiuyuan Wen Hongjing Zang |
| author_sort | Hongmei Zheng |
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| description | Abstract MicroRNA (miRNA) dysregulation has been identified in several carcinomas, including non-small cell lung cancer (NSCLC), and is known to play a role in the development and progression of this disease. We initially conducted a miRNA microarray analysis, which revealed that the MNK inhibitor CGP57380 increased the expression of miR-150-3p. A similar analysis was performed using data from The Cancer Genome Atlas (TCGA). Cell proliferation, colony formation and migration assays were validated in A549 and H157 cells treated with miR-150-3p mimics. Quantitative polymerase chain reaction (qPCR) was then used to detect potential target genes. We observed significant downregulation of miR-150-3p in NSCLC samples compared with normal samples (P = 0.035). High miR-150-3p expression was associated with longer overall survival (P = 0.005), as determined via a tissue microarray (TMA). These results were validated in the TCGA and revealed that miR-150-3p was expressed at low levels in NSCLC tissues (P < 0.0001) and that patients with high miR-150-3p expression had a better prognosis (P = 0.042). Moreover, the combination of miR-150-3p and CGP57380 exerted a synergistic inhibitory effect on colony formation, growth, and migration and induced apoptosis in NSCLC cell lines. We investigated the potential targets of miR-150-3p and successfully validated six potential target genes through qPCR analysis. High miR-150-3p expression may enhance the response to immunotherapy, cisplatin and gemcitabine. In summary, this study underscores the promising therapeutic implications of combining miR-150-3p and CGP57380 for NSCLC treatment. Additionally, this study provides valuable insights into the molecular mechanisms underlying the effects of this treatment. |
| format | Article |
| id | doaj-art-3142f96c2a244027b16b111213d0ca60 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Scientific Reports |
| spelling | doaj-art-3142f96c2a244027b16b111213d0ca602025-01-19T12:22:29ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-025-85793-7MicroRNA-150-3p enhances the antitumour effects of CGP57380 and is associated with a favourable prognosis in non-small cell lung cancerHongmei Zheng0Songqing Fan1Jiadi Luo2Qiuyuan Wen3Hongjing Zang4Department of Pathology, The Second Xiangya Hospital of Central South UniversityDepartment of Pathology, The Second Xiangya Hospital of Central South UniversityDepartment of Pathology, The Second Xiangya Hospital of Central South UniversityDepartment of Pathology, The Second Xiangya Hospital of Central South UniversityDepartment of Pathology, The Second Xiangya Hospital of Central South UniversityAbstract MicroRNA (miRNA) dysregulation has been identified in several carcinomas, including non-small cell lung cancer (NSCLC), and is known to play a role in the development and progression of this disease. We initially conducted a miRNA microarray analysis, which revealed that the MNK inhibitor CGP57380 increased the expression of miR-150-3p. A similar analysis was performed using data from The Cancer Genome Atlas (TCGA). Cell proliferation, colony formation and migration assays were validated in A549 and H157 cells treated with miR-150-3p mimics. Quantitative polymerase chain reaction (qPCR) was then used to detect potential target genes. We observed significant downregulation of miR-150-3p in NSCLC samples compared with normal samples (P = 0.035). High miR-150-3p expression was associated with longer overall survival (P = 0.005), as determined via a tissue microarray (TMA). These results were validated in the TCGA and revealed that miR-150-3p was expressed at low levels in NSCLC tissues (P < 0.0001) and that patients with high miR-150-3p expression had a better prognosis (P = 0.042). Moreover, the combination of miR-150-3p and CGP57380 exerted a synergistic inhibitory effect on colony formation, growth, and migration and induced apoptosis in NSCLC cell lines. We investigated the potential targets of miR-150-3p and successfully validated six potential target genes through qPCR analysis. High miR-150-3p expression may enhance the response to immunotherapy, cisplatin and gemcitabine. In summary, this study underscores the promising therapeutic implications of combining miR-150-3p and CGP57380 for NSCLC treatment. Additionally, this study provides valuable insights into the molecular mechanisms underlying the effects of this treatment.https://doi.org/10.1038/s41598-025-85793-7Non-small cell lung cancerMNK inhibitorMicroRNATargeted therapy |
| spellingShingle | Hongmei Zheng Songqing Fan Jiadi Luo Qiuyuan Wen Hongjing Zang MicroRNA-150-3p enhances the antitumour effects of CGP57380 and is associated with a favourable prognosis in non-small cell lung cancer Scientific Reports Non-small cell lung cancer MNK inhibitor MicroRNA Targeted therapy |
| title | MicroRNA-150-3p enhances the antitumour effects of CGP57380 and is associated with a favourable prognosis in non-small cell lung cancer |
| title_full | MicroRNA-150-3p enhances the antitumour effects of CGP57380 and is associated with a favourable prognosis in non-small cell lung cancer |
| title_fullStr | MicroRNA-150-3p enhances the antitumour effects of CGP57380 and is associated with a favourable prognosis in non-small cell lung cancer |
| title_full_unstemmed | MicroRNA-150-3p enhances the antitumour effects of CGP57380 and is associated with a favourable prognosis in non-small cell lung cancer |
| title_short | MicroRNA-150-3p enhances the antitumour effects of CGP57380 and is associated with a favourable prognosis in non-small cell lung cancer |
| title_sort | microrna 150 3p enhances the antitumour effects of cgp57380 and is associated with a favourable prognosis in non small cell lung cancer |
| topic | Non-small cell lung cancer MNK inhibitor MicroRNA Targeted therapy |
| url | https://doi.org/10.1038/s41598-025-85793-7 |
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