Immunological Dynamics Associated with Direct-Acting Antiviral Therapies in Naive and Experimented HCV Chronic-Infected Patients
The therapeutic strategies used in the treatment of hepatitis C are essentially based on the combination of direct-acting antiviral agents (DAAs). This therapy has been shown to be very effective in relation to patient adherence to treatment and has shown high rates of sustained virological response...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/4738237 |
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| author | Grenda Leite Pereira Andréa Monteiro Tarragô Walter Luiz Lima Neves Pedro Vieira da Silva Neto Priscila Sarmento de Souza Juliana dos Santos Affonso Keyla Santos de Sousa Jéssica Albuquerque da Silva Allyson Guimarães Costa Flamir da Silva Victoria Marilu Barbieri Victoria Adriana Malheiro |
| author_facet | Grenda Leite Pereira Andréa Monteiro Tarragô Walter Luiz Lima Neves Pedro Vieira da Silva Neto Priscila Sarmento de Souza Juliana dos Santos Affonso Keyla Santos de Sousa Jéssica Albuquerque da Silva Allyson Guimarães Costa Flamir da Silva Victoria Marilu Barbieri Victoria Adriana Malheiro |
| author_sort | Grenda Leite Pereira |
| collection | DOAJ |
| description | The therapeutic strategies used in the treatment of hepatitis C are essentially based on the combination of direct-acting antiviral agents (DAAs). This therapy has been shown to be very effective in relation to patient adherence to treatment and has shown high rates of sustained virological response (SVR). However, the immunological dynamics of patients infected with HCV is poorly understood. This fact led us to investigate the immune system of naive and experienced patients, who we followed before the therapy and three months after the end of treatment. In this study, 35 naive and experienced Brazilian patients with chronic hepatitis C and 50 healthy donors (HD group) were studied. The analysis of the soluble immunological biomarkers was performed using the flow cytometry methodology. The SVR rate was >90% among the 35 patients. Before treatment, correlations in the naive HCV group demonstrated a mix of inflammatory response occurring with moderate correlations between chemokines, inflammatory cytokines, and Th2 profile, with a strong regulation between IL-10 and IL-17A. On the other hand, experienced patients demonstrated a poor interaction between cytokines, chemokines, and cells with a strong correlation between IL-10, IL-6, CXCL-10, and CD8+ besides the interactions between IFN-γ and IL-4. Furthermore, naive and experienced patients seem to have a distinct soluble biomarker profile; therefore, a long-term follow-up is needed to evaluate patients treated with DAAs. |
| format | Article |
| id | doaj-art-313e10d7a2c2466baca70c2962c10288 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-313e10d7a2c2466baca70c2962c102882025-02-03T01:11:29ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/47382374738237Immunological Dynamics Associated with Direct-Acting Antiviral Therapies in Naive and Experimented HCV Chronic-Infected PatientsGrenda Leite Pereira0Andréa Monteiro Tarragô1Walter Luiz Lima Neves2Pedro Vieira da Silva Neto3Priscila Sarmento de Souza4Juliana dos Santos Affonso5Keyla Santos de Sousa6Jéssica Albuquerque da Silva7Allyson Guimarães Costa8Flamir da Silva Victoria9Marilu Barbieri Victoria10Adriana Malheiro11Universidade Federal do Amazonas (UFAM), Manaus, AM, BrazilUniversidade Federal do Amazonas (UFAM), Manaus, AM, BrazilUniversidade Federal do Amazonas (UFAM), Manaus, AM, BrazilUniversidade Federal do Amazonas (UFAM), Manaus, AM, BrazilUniversidade Federal do Amazonas (UFAM), Manaus, AM, BrazilFundação Hospitalar de Hematologia e Hemoterapia do Amazonas (HEMOAM), Manaus, AM, BrazilFundação Hospitalar de Hematologia e Hemoterapia do Amazonas (HEMOAM), Manaus, AM, BrazilFundação Hospitalar de Hematologia e Hemoterapia do Amazonas (HEMOAM), Manaus, AM, BrazilUniversidade Federal do Amazonas (UFAM), Manaus, AM, BrazilUniversidade do Estado do Amazonas (UEA), Manaus, AM, BrazilUniversidade do Estado do Amazonas (UEA), Manaus, AM, BrazilUniversidade Federal do Amazonas (UFAM), Manaus, AM, BrazilThe therapeutic strategies used in the treatment of hepatitis C are essentially based on the combination of direct-acting antiviral agents (DAAs). This therapy has been shown to be very effective in relation to patient adherence to treatment and has shown high rates of sustained virological response (SVR). However, the immunological dynamics of patients infected with HCV is poorly understood. This fact led us to investigate the immune system of naive and experienced patients, who we followed before the therapy and three months after the end of treatment. In this study, 35 naive and experienced Brazilian patients with chronic hepatitis C and 50 healthy donors (HD group) were studied. The analysis of the soluble immunological biomarkers was performed using the flow cytometry methodology. The SVR rate was >90% among the 35 patients. Before treatment, correlations in the naive HCV group demonstrated a mix of inflammatory response occurring with moderate correlations between chemokines, inflammatory cytokines, and Th2 profile, with a strong regulation between IL-10 and IL-17A. On the other hand, experienced patients demonstrated a poor interaction between cytokines, chemokines, and cells with a strong correlation between IL-10, IL-6, CXCL-10, and CD8+ besides the interactions between IFN-γ and IL-4. Furthermore, naive and experienced patients seem to have a distinct soluble biomarker profile; therefore, a long-term follow-up is needed to evaluate patients treated with DAAs.http://dx.doi.org/10.1155/2019/4738237 |
| spellingShingle | Grenda Leite Pereira Andréa Monteiro Tarragô Walter Luiz Lima Neves Pedro Vieira da Silva Neto Priscila Sarmento de Souza Juliana dos Santos Affonso Keyla Santos de Sousa Jéssica Albuquerque da Silva Allyson Guimarães Costa Flamir da Silva Victoria Marilu Barbieri Victoria Adriana Malheiro Immunological Dynamics Associated with Direct-Acting Antiviral Therapies in Naive and Experimented HCV Chronic-Infected Patients Mediators of Inflammation |
| title | Immunological Dynamics Associated with Direct-Acting Antiviral Therapies in Naive and Experimented HCV Chronic-Infected Patients |
| title_full | Immunological Dynamics Associated with Direct-Acting Antiviral Therapies in Naive and Experimented HCV Chronic-Infected Patients |
| title_fullStr | Immunological Dynamics Associated with Direct-Acting Antiviral Therapies in Naive and Experimented HCV Chronic-Infected Patients |
| title_full_unstemmed | Immunological Dynamics Associated with Direct-Acting Antiviral Therapies in Naive and Experimented HCV Chronic-Infected Patients |
| title_short | Immunological Dynamics Associated with Direct-Acting Antiviral Therapies in Naive and Experimented HCV Chronic-Infected Patients |
| title_sort | immunological dynamics associated with direct acting antiviral therapies in naive and experimented hcv chronic infected patients |
| url | http://dx.doi.org/10.1155/2019/4738237 |
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