Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor Precursor

Commercial lipases, from porcine pancreas (PPL), Candida rugosa (CRL), and Thermomyces lanuginosus (Lipozyme TL IM), were investigated in terms of their efficiency for the hydrolysis of safflower oil (SO) for the liberation of free linoleic acid (LA), used as a flavor precursor. Although PPL, under...

Full description

Saved in:
Bibliographic Details
Main Authors: Marya Aziz, Florence Husson, Selim Kermasha
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:International Journal of Food Science
Online Access:http://dx.doi.org/10.1155/2015/594238
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832565086672125952
author Marya Aziz
Florence Husson
Selim Kermasha
author_facet Marya Aziz
Florence Husson
Selim Kermasha
author_sort Marya Aziz
collection DOAJ
description Commercial lipases, from porcine pancreas (PPL), Candida rugosa (CRL), and Thermomyces lanuginosus (Lipozyme TL IM), were investigated in terms of their efficiency for the hydrolysis of safflower oil (SO) for the liberation of free linoleic acid (LA), used as a flavor precursor. Although PPL, under the optimized conditions, showed a high degree of hydrolysis (91.6%), its low tolerance towards higher substrate concentrations could limit its use for SO hydrolysis. In comparison to the other investigated lipases, Lipozyme TL IM required higher amount of enzyme and an additional 3 h of reaction time to achieve its maximum degree of SO hydrolysis (90.2%). On the basis of the experimental findings, CRL was selected as the most appropriate biocatalyst, with 84.1% degree of hydrolysis. The chromatographic analyses showed that the CRL-hydrolyzed SO is composed mainly of free LA.
format Article
id doaj-art-310b82f54c54492a957eb9b91e5a3299
institution Kabale University
issn 2356-7015
2314-5765
language English
publishDate 2015-01-01
publisher Wiley
record_format Article
series International Journal of Food Science
spelling doaj-art-310b82f54c54492a957eb9b91e5a32992025-02-03T01:09:13ZengWileyInternational Journal of Food Science2356-70152314-57652015-01-01201510.1155/2015/594238594238Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor PrecursorMarya Aziz0Florence Husson1Selim Kermasha2Department of Food Science and Agricultural Chemistry, McGill University, 21111 Lakeshore, Sainte-Anne-de-Bellevue, QC, H9X 3V9, CanadaAgroSup Dijon, Université de Bourgogne, 1 Esplanade Erasme, 21000 Dijon, FranceDepartment of Food Science and Agricultural Chemistry, McGill University, 21111 Lakeshore, Sainte-Anne-de-Bellevue, QC, H9X 3V9, CanadaCommercial lipases, from porcine pancreas (PPL), Candida rugosa (CRL), and Thermomyces lanuginosus (Lipozyme TL IM), were investigated in terms of their efficiency for the hydrolysis of safflower oil (SO) for the liberation of free linoleic acid (LA), used as a flavor precursor. Although PPL, under the optimized conditions, showed a high degree of hydrolysis (91.6%), its low tolerance towards higher substrate concentrations could limit its use for SO hydrolysis. In comparison to the other investigated lipases, Lipozyme TL IM required higher amount of enzyme and an additional 3 h of reaction time to achieve its maximum degree of SO hydrolysis (90.2%). On the basis of the experimental findings, CRL was selected as the most appropriate biocatalyst, with 84.1% degree of hydrolysis. The chromatographic analyses showed that the CRL-hydrolyzed SO is composed mainly of free LA.http://dx.doi.org/10.1155/2015/594238
spellingShingle Marya Aziz
Florence Husson
Selim Kermasha
Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor Precursor
International Journal of Food Science
title Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor Precursor
title_full Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor Precursor
title_fullStr Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor Precursor
title_full_unstemmed Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor Precursor
title_short Optimization of the Hydrolysis of Safflower Oil for the Production of Linoleic Acid, Used as Flavor Precursor
title_sort optimization of the hydrolysis of safflower oil for the production of linoleic acid used as flavor precursor
url http://dx.doi.org/10.1155/2015/594238
work_keys_str_mv AT maryaaziz optimizationofthehydrolysisofsaffloweroilfortheproductionoflinoleicacidusedasflavorprecursor
AT florencehusson optimizationofthehydrolysisofsaffloweroilfortheproductionoflinoleicacidusedasflavorprecursor
AT selimkermasha optimizationofthehydrolysisofsaffloweroilfortheproductionoflinoleicacidusedasflavorprecursor