RT-PCR method for selective detection of silent gene transcripts in silencing mutants in homothallic strains of Schizosaccharomyces pombe
Here we describe a method that allows selective detection of silent copy transcripts in homothallic strains of Schizosaccharomyces pombe in the presence of the active cassettes. The method involving RT-PCR (reverse transcriptase polymerase chain reaction) exploits our observation that the silent cop...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2008-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112671 |
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