The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples
Background. Shiga toxin-producing Escherichia coli (STEC) is known as a crucial zoonotic food-borne pathogen. A total of 257 raw chicken meat samples were collected from different markets in Hamadan, west of Iran, from January 2016 to May 2017. Materials and Methods. The samples were cultured in sel...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/3333240 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547209057402880 |
|---|---|
| author | Omid Zarei Leili Shokoohizadeh Hadi Hossainpour Mohammad Yousef Alikhani |
| author_facet | Omid Zarei Leili Shokoohizadeh Hadi Hossainpour Mohammad Yousef Alikhani |
| author_sort | Omid Zarei |
| collection | DOAJ |
| description | Background. Shiga toxin-producing Escherichia coli (STEC) is known as a crucial zoonotic food-borne pathogen. A total of 257 raw chicken meat samples were collected from different markets in Hamadan, west of Iran, from January 2016 to May 2017. Materials and Methods. The samples were cultured in selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by the disk diffusion method. The genetic relatedness of the E. coli O157 isolates was analyzed by ERIC-PCR. Results. In total, 93 (36% ± 3.12) of the isolates were identified as E. coli in this study. Based on serological and microbiological tests, 36 (38.7% ± 9.9), 7 (7.5% ± 5.35), and 12 (12.9% ± 6.81) of the E. coli isolates were characterized as STEC, enteropathogenic E. coli (EPEC), and attaching and effacing E. coli (AEEC) strains, respectively. A high level of resistance to nalidixic acid (91.4% ± 5.7), tetracycline (89.2% ± 6.31), ampicillin (82.8% ± 7.67), and trimotoprime-sulfametoxazole (71% ± 9.22) was detected among the E. coli isolates. The analysis of the ERIC-PCR results showed five different ERIC types among the E. coli O157 isolates. Conclusions. Based on our findings, control and check-up of poultry meats should be considered as a crucial issue for public health. |
| format | Article |
| id | doaj-art-31063d8254444d4db530974b55445255 |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-31063d8254444d4db530974b554452552025-02-03T06:45:28ZengWileyInternational Journal of Microbiology1687-91982021-01-01202110.1155/2021/3333240The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat SamplesOmid Zarei0Leili Shokoohizadeh1Hadi Hossainpour2Mohammad Yousef Alikhani3Student Research CommitteeDepartment of MicrobiologyStudent Research CommitteeDepartment of MicrobiologyBackground. Shiga toxin-producing Escherichia coli (STEC) is known as a crucial zoonotic food-borne pathogen. A total of 257 raw chicken meat samples were collected from different markets in Hamadan, west of Iran, from January 2016 to May 2017. Materials and Methods. The samples were cultured in selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by the disk diffusion method. The genetic relatedness of the E. coli O157 isolates was analyzed by ERIC-PCR. Results. In total, 93 (36% ± 3.12) of the isolates were identified as E. coli in this study. Based on serological and microbiological tests, 36 (38.7% ± 9.9), 7 (7.5% ± 5.35), and 12 (12.9% ± 6.81) of the E. coli isolates were characterized as STEC, enteropathogenic E. coli (EPEC), and attaching and effacing E. coli (AEEC) strains, respectively. A high level of resistance to nalidixic acid (91.4% ± 5.7), tetracycline (89.2% ± 6.31), ampicillin (82.8% ± 7.67), and trimotoprime-sulfametoxazole (71% ± 9.22) was detected among the E. coli isolates. The analysis of the ERIC-PCR results showed five different ERIC types among the E. coli O157 isolates. Conclusions. Based on our findings, control and check-up of poultry meats should be considered as a crucial issue for public health.http://dx.doi.org/10.1155/2021/3333240 |
| spellingShingle | Omid Zarei Leili Shokoohizadeh Hadi Hossainpour Mohammad Yousef Alikhani The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples International Journal of Microbiology |
| title | The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples |
| title_full | The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples |
| title_fullStr | The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples |
| title_full_unstemmed | The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples |
| title_short | The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples |
| title_sort | prevalence of shiga toxin producing escherichia coli and enteropathogenic escherichia coli isolated from raw chicken meat samples |
| url | http://dx.doi.org/10.1155/2021/3333240 |
| work_keys_str_mv | AT omidzarei theprevalenceofshigatoxinproducingescherichiacoliandenteropathogenicescherichiacoliisolatedfromrawchickenmeatsamples AT leilishokoohizadeh theprevalenceofshigatoxinproducingescherichiacoliandenteropathogenicescherichiacoliisolatedfromrawchickenmeatsamples AT hadihossainpour theprevalenceofshigatoxinproducingescherichiacoliandenteropathogenicescherichiacoliisolatedfromrawchickenmeatsamples AT mohammadyousefalikhani theprevalenceofshigatoxinproducingescherichiacoliandenteropathogenicescherichiacoliisolatedfromrawchickenmeatsamples AT omidzarei prevalenceofshigatoxinproducingescherichiacoliandenteropathogenicescherichiacoliisolatedfromrawchickenmeatsamples AT leilishokoohizadeh prevalenceofshigatoxinproducingescherichiacoliandenteropathogenicescherichiacoliisolatedfromrawchickenmeatsamples AT hadihossainpour prevalenceofshigatoxinproducingescherichiacoliandenteropathogenicescherichiacoliisolatedfromrawchickenmeatsamples AT mohammadyousefalikhani prevalenceofshigatoxinproducingescherichiacoliandenteropathogenicescherichiacoliisolatedfromrawchickenmeatsamples |