Purification and Characterization of Hemagglutinating Proteins from Poker-Chip Venus (Meretrix lusoria) and Corbicula Clam (Corbicula fluminea)
Hemagglutinating proteins (HAPs) were purified from Poker-chip Venus (Meretrix lusoria) and Corbicula clam (Corbicula fluminea) using gel-filtration chromatography on a Sephacryl S-300 column. The molecular weights of the HAPs obtained from Poker-chip Venus and Corbicula clam were 358 kDa and 380 kD...
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| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/906737 |
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| author | Chin-Fu Cheng Shao-Wen Hung Yung-Chung Chang Ming-Hui Chen Chen-Hsuan Chang Li-Tse Tsou Ching-Yu Tu Yu-Hsing Lin Pan-Chen Liu Shiun-Long Lin Way-Shyan Wang |
| author_facet | Chin-Fu Cheng Shao-Wen Hung Yung-Chung Chang Ming-Hui Chen Chen-Hsuan Chang Li-Tse Tsou Ching-Yu Tu Yu-Hsing Lin Pan-Chen Liu Shiun-Long Lin Way-Shyan Wang |
| author_sort | Chin-Fu Cheng |
| collection | DOAJ |
| description | Hemagglutinating proteins (HAPs) were purified from Poker-chip Venus (Meretrix lusoria) and Corbicula clam (Corbicula fluminea) using gel-filtration chromatography on a Sephacryl S-300 column. The molecular weights of the HAPs obtained from Poker-chip Venus and Corbicula clam were 358 kDa and 380 kDa, respectively. Purified HAP from Poker-chip Venus yielded two subunits with molecular weights of 26 kDa and 29 kDa. However, only one HAP subunit was purified from Corbicula clam, and its molecular weight was 32 kDa. The two Poker-chip Venus HAPs possessed hemagglutinating ability (HAA) for erythrocytes of some vertebrate animal species, especially tilapia. Moreover, HAA of the HAP purified from Poker-chip Venus was higher than that of the HAP of Corbicula clam. Furthermore, Poker-chip Venus HAPs possessed better HAA at a pH higher than 7.0. When the temperature was at 4°C–10°C or the salinity was less than 0.5‰, the two Poker-chip Venus HAPs possessed better HAA compared with that of Corbicula clam. |
| format | Article |
| id | doaj-art-30fbde295aed4c739ecc8e55f0120a08 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-30fbde295aed4c739ecc8e55f0120a082025-02-03T05:57:49ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/906737906737Purification and Characterization of Hemagglutinating Proteins from Poker-Chip Venus (Meretrix lusoria) and Corbicula Clam (Corbicula fluminea)Chin-Fu Cheng0Shao-Wen Hung1Yung-Chung Chang2Ming-Hui Chen3Chen-Hsuan Chang4Li-Tse Tsou5Ching-Yu Tu6Yu-Hsing Lin7Pan-Chen Liu8Shiun-Long Lin9Way-Shyan Wang10Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, TaiwanAgricultural Biotechnology Research Center, Academia Sinica, Taipei 115, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, TaiwanAgricultural Chemicals and Toxic Substances Research Institute, Council of Agriculture, Executive Yuan, Taichung 413, TaiwanNursing Department, Yuanpei University, Hsinchu 300, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, TaiwanDepartment of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung 402, TaiwanHemagglutinating proteins (HAPs) were purified from Poker-chip Venus (Meretrix lusoria) and Corbicula clam (Corbicula fluminea) using gel-filtration chromatography on a Sephacryl S-300 column. The molecular weights of the HAPs obtained from Poker-chip Venus and Corbicula clam were 358 kDa and 380 kDa, respectively. Purified HAP from Poker-chip Venus yielded two subunits with molecular weights of 26 kDa and 29 kDa. However, only one HAP subunit was purified from Corbicula clam, and its molecular weight was 32 kDa. The two Poker-chip Venus HAPs possessed hemagglutinating ability (HAA) for erythrocytes of some vertebrate animal species, especially tilapia. Moreover, HAA of the HAP purified from Poker-chip Venus was higher than that of the HAP of Corbicula clam. Furthermore, Poker-chip Venus HAPs possessed better HAA at a pH higher than 7.0. When the temperature was at 4°C–10°C or the salinity was less than 0.5‰, the two Poker-chip Venus HAPs possessed better HAA compared with that of Corbicula clam.http://dx.doi.org/10.1100/2012/906737 |
| spellingShingle | Chin-Fu Cheng Shao-Wen Hung Yung-Chung Chang Ming-Hui Chen Chen-Hsuan Chang Li-Tse Tsou Ching-Yu Tu Yu-Hsing Lin Pan-Chen Liu Shiun-Long Lin Way-Shyan Wang Purification and Characterization of Hemagglutinating Proteins from Poker-Chip Venus (Meretrix lusoria) and Corbicula Clam (Corbicula fluminea) The Scientific World Journal |
| title | Purification and Characterization of Hemagglutinating Proteins from Poker-Chip Venus (Meretrix lusoria) and Corbicula Clam (Corbicula fluminea) |
| title_full | Purification and Characterization of Hemagglutinating Proteins from Poker-Chip Venus (Meretrix lusoria) and Corbicula Clam (Corbicula fluminea) |
| title_fullStr | Purification and Characterization of Hemagglutinating Proteins from Poker-Chip Venus (Meretrix lusoria) and Corbicula Clam (Corbicula fluminea) |
| title_full_unstemmed | Purification and Characterization of Hemagglutinating Proteins from Poker-Chip Venus (Meretrix lusoria) and Corbicula Clam (Corbicula fluminea) |
| title_short | Purification and Characterization of Hemagglutinating Proteins from Poker-Chip Venus (Meretrix lusoria) and Corbicula Clam (Corbicula fluminea) |
| title_sort | purification and characterization of hemagglutinating proteins from poker chip venus meretrix lusoria and corbicula clam corbicula fluminea |
| url | http://dx.doi.org/10.1100/2012/906737 |
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