Applicability of TB-LAMP test for diagnosis of pulmonary TB among HIV-positive individuals
BACKGROUND: The loop-mediated isothermal amplification for TB (TB-LAMP) assay is more cost-effective and accessible than the Xpert ® MTB/RIF assay. This study aimed to evaluate the diagnostic performance of the TB-LAMP assay in individuals with and without HIV infection. METHODS: Patients aged ≥15 y...
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International Union Against Tuberculosis and Lung Disease (The Union)
2025-01-01
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| Series: | IJTLD Open |
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| Online Access: | https://www.ingentaconnect.com/contentone/iuatld/ijtldo/2025/00000002/00000001/art00006 |
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| author | B. Muriithi M.M. Bundi T. Moriyasu A. Ahmed M. Mwamzuka M. Karama S. Kaneko |
| author_facet | B. Muriithi M.M. Bundi T. Moriyasu A. Ahmed M. Mwamzuka M. Karama S. Kaneko |
| author_sort | B. Muriithi |
| collection | DOAJ |
| description | BACKGROUND: The loop-mediated isothermal amplification for TB (TB-LAMP) assay is more cost-effective and accessible than the Xpert ® MTB/RIF assay. This study aimed to evaluate the diagnostic performance of the TB-LAMP assay in individuals with and without HIV infection. METHODS: Patients aged ≥15 years presenting with symptoms of TB were included in the study. The TB-LAMP assay was performed alongside routine TB diagnostic methods, including the Xpert assay and smear microscopy, to evaluate discrepancies in test results and associated factors. RESULTS: A total of 903 patients were enrolled in the study. The positive percentage agreement for smear microscopy and TB-LAMP compared with the Xpert assay was respectively 54.3% (95% CI 46.6–61.8) and 76.6% (95% CI 69.9–82.6). Among HIV-positive individuals, the positive percentage agreement was 42.5% (95% CI 32.9–52.4) for smear microscopy and 68.9% (95% CI 59.1–77.5) for TB-LAMP. Factors such as age >60 years (adjusted OR 3.29, 95% CI 0.32–33.83), loss of appetite (aOR 0.30, 95% CI 0.13–0.70), and HIV-positive status (aOR 3.29, 95% CI 1.12–9.63) were associated with discrepancies between TB-LAMP and Xpert results. CONCLUSIONS: TB-LAMP demonstrated better agreement with the Xpert assay compared with smear microscopy in detecting TB among HIV-positive patients, suggesting that TB-LAMP could effectively replace smear microscopy. |
| format | Article |
| id | doaj-art-30de87d446b1480e9366859e0760fd26 |
| institution | Kabale University |
| issn | 3005-7590 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | International Union Against Tuberculosis and Lung Disease (The Union) |
| record_format | Article |
| series | IJTLD Open |
| spelling | doaj-art-30de87d446b1480e9366859e0760fd262025-01-23T13:39:30ZengInternational Union Against Tuberculosis and Lung Disease (The Union)IJTLD Open3005-75902025-01-0121263210.5588/ijtldopen.24.04106Applicability of TB-LAMP test for diagnosis of pulmonary TB among HIV-positive individualsB. Muriithi0M.M. Bundi1T. Moriyasu2A. Ahmed3M. Mwamzuka4M. Karama5S. Kaneko6Nairobi Research Station, Nagasaki University Institute of Tropical Medicine (NUITM)-Kenya Medical Research Institute (KEMRI) Project, Nairobi, Kenya;Kenya Medical Research Institute, KEMRI Graduate School, Nairobi, Kenya;Office for International Relations, Nagasaki University, Nagasaki, Japan;Bomu Hospital, Mombasa, Kenya;Bomu Hospital, Mombasa, Kenya;African Medical and Research Foundation (AMREF) Health Africa Ethics and Scientific Research Committee, AMREF Health Africa, Nairobi, Kenya;Nairobi Research Station, Nagasaki University Institute of Tropical Medicine (NUITM)-Kenya Medical Research Institute (KEMRI) Project, Nairobi, Kenya;BACKGROUND: The loop-mediated isothermal amplification for TB (TB-LAMP) assay is more cost-effective and accessible than the Xpert ® MTB/RIF assay. This study aimed to evaluate the diagnostic performance of the TB-LAMP assay in individuals with and without HIV infection. METHODS: Patients aged ≥15 years presenting with symptoms of TB were included in the study. The TB-LAMP assay was performed alongside routine TB diagnostic methods, including the Xpert assay and smear microscopy, to evaluate discrepancies in test results and associated factors. RESULTS: A total of 903 patients were enrolled in the study. The positive percentage agreement for smear microscopy and TB-LAMP compared with the Xpert assay was respectively 54.3% (95% CI 46.6–61.8) and 76.6% (95% CI 69.9–82.6). Among HIV-positive individuals, the positive percentage agreement was 42.5% (95% CI 32.9–52.4) for smear microscopy and 68.9% (95% CI 59.1–77.5) for TB-LAMP. Factors such as age >60 years (adjusted OR 3.29, 95% CI 0.32–33.83), loss of appetite (aOR 0.30, 95% CI 0.13–0.70), and HIV-positive status (aOR 3.29, 95% CI 1.12–9.63) were associated with discrepancies between TB-LAMP and Xpert results. CONCLUSIONS: TB-LAMP demonstrated better agreement with the Xpert assay compared with smear microscopy in detecting TB among HIV-positive patients, suggesting that TB-LAMP could effectively replace smear microscopy.https://www.ingentaconnect.com/contentone/iuatld/ijtldo/2025/00000002/00000001/art00006diagnosishivtuberculosispulmonary tbloop-mediated isothermal amplification |
| spellingShingle | B. Muriithi M.M. Bundi T. Moriyasu A. Ahmed M. Mwamzuka M. Karama S. Kaneko Applicability of TB-LAMP test for diagnosis of pulmonary TB among HIV-positive individuals IJTLD Open diagnosis hiv tuberculosis pulmonary tb loop-mediated isothermal amplification |
| title | Applicability of TB-LAMP test for diagnosis of pulmonary TB among HIV-positive individuals |
| title_full | Applicability of TB-LAMP test for diagnosis of pulmonary TB among HIV-positive individuals |
| title_fullStr | Applicability of TB-LAMP test for diagnosis of pulmonary TB among HIV-positive individuals |
| title_full_unstemmed | Applicability of TB-LAMP test for diagnosis of pulmonary TB among HIV-positive individuals |
| title_short | Applicability of TB-LAMP test for diagnosis of pulmonary TB among HIV-positive individuals |
| title_sort | applicability of tb lamp test for diagnosis of pulmonary tb among hiv positive individuals |
| topic | diagnosis hiv tuberculosis pulmonary tb loop-mediated isothermal amplification |
| url | https://www.ingentaconnect.com/contentone/iuatld/ijtldo/2025/00000002/00000001/art00006 |
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