Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression
As shown in our previous studies, growth and metastasis of ovarian cancer can be regulated by adipose-derived mesenchymal stem cells (ADSCs). However, the underlying mechanism has not yet been revealed. In this study, a proteomics analysis was performed to compare protein expression treated with and...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/9037197 |
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| author | Yijing Chu Min You Jingjing Zhang Guoqiang Gao Rendong Han Wenqiang Luo Tingting Liu Jianxin Zuo Fuling Wang |
| author_facet | Yijing Chu Min You Jingjing Zhang Guoqiang Gao Rendong Han Wenqiang Luo Tingting Liu Jianxin Zuo Fuling Wang |
| author_sort | Yijing Chu |
| collection | DOAJ |
| description | As shown in our previous studies, growth and metastasis of ovarian cancer can be regulated by adipose-derived mesenchymal stem cells (ADSCs). However, the underlying mechanism has not yet been revealed. In this study, a proteomics analysis was performed to compare protein expression treated with and without ADSCs in ovarian cancer cells. Protein levels were altered in ovarian cancer cells due to the treatment of ADSCs. Thymosin beta 4 X-linked (TMSB4X) levels changed dramatically, and this protein was identified as one of the most important candidate molecules contributing to the tumour-promoting effects of ADSCs. Compared with the cells that are cultured in the normal growth medium, the TMSB4X levels cultured in ADSC-conditioned medium increased significantly in ovarian cancer cells. Furthermore, the growth and invasion of cancer cells were decreased, even in the ADSC-conditioned medium treatment group (P<0.05), by the inhibition of TMSB4X. As shown in the bioluminescence images captured in vivo, increased ovarian cancer’s growth and metastasis, along with elevated TMSB4X expression, were observed in the group of ADSC-conditioned medium, and the tumour-promoting effect of ADSCs was attenuated by the inhibition of TMSB4X. Based on our findings, increased TMSB4X expression may play a role in accelerating the ADSC-mediated proliferation, invasion, and migration of ovarian cancers. |
| format | Article |
| id | doaj-art-308732b559434c6fa77bd6d78f35dab8 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-308732b559434c6fa77bd6d78f35dab82025-02-03T05:58:05ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/90371979037197Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked ExpressionYijing Chu0Min You1Jingjing Zhang2Guoqiang Gao3Rendong Han4Wenqiang Luo5Tingting Liu6Jianxin Zuo7Fuling Wang8Department of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics and Gynaecology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaAs shown in our previous studies, growth and metastasis of ovarian cancer can be regulated by adipose-derived mesenchymal stem cells (ADSCs). However, the underlying mechanism has not yet been revealed. In this study, a proteomics analysis was performed to compare protein expression treated with and without ADSCs in ovarian cancer cells. Protein levels were altered in ovarian cancer cells due to the treatment of ADSCs. Thymosin beta 4 X-linked (TMSB4X) levels changed dramatically, and this protein was identified as one of the most important candidate molecules contributing to the tumour-promoting effects of ADSCs. Compared with the cells that are cultured in the normal growth medium, the TMSB4X levels cultured in ADSC-conditioned medium increased significantly in ovarian cancer cells. Furthermore, the growth and invasion of cancer cells were decreased, even in the ADSC-conditioned medium treatment group (P<0.05), by the inhibition of TMSB4X. As shown in the bioluminescence images captured in vivo, increased ovarian cancer’s growth and metastasis, along with elevated TMSB4X expression, were observed in the group of ADSC-conditioned medium, and the tumour-promoting effect of ADSCs was attenuated by the inhibition of TMSB4X. Based on our findings, increased TMSB4X expression may play a role in accelerating the ADSC-mediated proliferation, invasion, and migration of ovarian cancers.http://dx.doi.org/10.1155/2019/9037197 |
| spellingShingle | Yijing Chu Min You Jingjing Zhang Guoqiang Gao Rendong Han Wenqiang Luo Tingting Liu Jianxin Zuo Fuling Wang Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression Stem Cells International |
| title | Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression |
| title_full | Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression |
| title_fullStr | Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression |
| title_full_unstemmed | Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression |
| title_short | Adipose-Derived Mesenchymal Stem Cells Enhance Ovarian Cancer Growth and Metastasis by Increasing Thymosin Beta 4X-Linked Expression |
| title_sort | adipose derived mesenchymal stem cells enhance ovarian cancer growth and metastasis by increasing thymosin beta 4x linked expression |
| url | http://dx.doi.org/10.1155/2019/9037197 |
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