Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA
Electrochemical behavior of the anticancer drug doxorubicin hydrochloride was studied using cyclic voltammetry in aqueous medium using Hepes buffer (pH~7.4). At this pH, doxorubicin hydrochloride undergoes a reversible two-electron reduction with E1/2 value −665±5 mV (versus Ag/AgCl, saturated KCl)....
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/517371 |
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| author | Partha Sarathi Guin Saurabh Das |
| author_facet | Partha Sarathi Guin Saurabh Das |
| author_sort | Partha Sarathi Guin |
| collection | DOAJ |
| description | Electrochemical behavior of the anticancer drug doxorubicin hydrochloride was studied using cyclic voltammetry in aqueous medium using Hepes buffer (pH~7.4). At this pH, doxorubicin hydrochloride undergoes a reversible two-electron reduction with E1/2 value −665±5 mV (versus Ag/AgCl, saturated KCl). Depending on scan rates, processes were either quasireversible (at low scan rates) or near perfect reversible (at high scan rates). This difference in behavior of doxorubicin hydrochloride with scan rate studied over the same potential range speaks of differences in electron transfer processes in doxorubicin hydrochloride. Attempt was made to identify and understand the species involved using simulation. The information obtained was used to study the interaction of doxorubicin hydrochloride with calf thymus DNA. Cathodic peak current gradually decreased as more calf thymus DNA was added. The decrease in cathodic peak current was used to estimate the interaction of the drug with calf thymus DNA. Nonlinear curve fit analysis was applied to evaluate the intrinsic binding constant and site size of interaction that was compared with previous results on doxorubicin hydrochloride-DNA interaction monitored by cyclic voltammetry or spectroscopic techniques. |
| format | Article |
| id | doaj-art-306fa89fa7df4f229a9c5f297be64ce0 |
| institution | Kabale University |
| issn | 2090-3529 2090-3537 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | International Journal of Electrochemistry |
| spelling | doaj-art-306fa89fa7df4f229a9c5f297be64ce02025-02-03T01:28:47ZengWileyInternational Journal of Electrochemistry2090-35292090-35372014-01-01201410.1155/2014/517371517371Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNAPartha Sarathi Guin0Saurabh Das1Department of Chemistry, Shibpur Dinobundhoo Institution (College), 412/1 G. T. Road (South), Howrah 711102, IndiaDepartment of Chemistry, Jadavpur University, Raja SC Mullick Road, Kolkata 700032, IndiaElectrochemical behavior of the anticancer drug doxorubicin hydrochloride was studied using cyclic voltammetry in aqueous medium using Hepes buffer (pH~7.4). At this pH, doxorubicin hydrochloride undergoes a reversible two-electron reduction with E1/2 value −665±5 mV (versus Ag/AgCl, saturated KCl). Depending on scan rates, processes were either quasireversible (at low scan rates) or near perfect reversible (at high scan rates). This difference in behavior of doxorubicin hydrochloride with scan rate studied over the same potential range speaks of differences in electron transfer processes in doxorubicin hydrochloride. Attempt was made to identify and understand the species involved using simulation. The information obtained was used to study the interaction of doxorubicin hydrochloride with calf thymus DNA. Cathodic peak current gradually decreased as more calf thymus DNA was added. The decrease in cathodic peak current was used to estimate the interaction of the drug with calf thymus DNA. Nonlinear curve fit analysis was applied to evaluate the intrinsic binding constant and site size of interaction that was compared with previous results on doxorubicin hydrochloride-DNA interaction monitored by cyclic voltammetry or spectroscopic techniques.http://dx.doi.org/10.1155/2014/517371 |
| spellingShingle | Partha Sarathi Guin Saurabh Das Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA International Journal of Electrochemistry |
| title | Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA |
| title_full | Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA |
| title_fullStr | Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA |
| title_full_unstemmed | Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA |
| title_short | Exploration of Electrochemical Intermediates of the Anticancer Drug Doxorubicin Hydrochloride Using Cyclic Voltammetry and Simulation Studies with an Evaluation for Its Interaction with DNA |
| title_sort | exploration of electrochemical intermediates of the anticancer drug doxorubicin hydrochloride using cyclic voltammetry and simulation studies with an evaluation for its interaction with dna |
| url | http://dx.doi.org/10.1155/2014/517371 |
| work_keys_str_mv | AT parthasarathiguin explorationofelectrochemicalintermediatesoftheanticancerdrugdoxorubicinhydrochlorideusingcyclicvoltammetryandsimulationstudieswithanevaluationforitsinteractionwithdna AT saurabhdas explorationofelectrochemicalintermediatesoftheanticancerdrugdoxorubicinhydrochlorideusingcyclicvoltammetryandsimulationstudieswithanevaluationforitsinteractionwithdna |