A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles
Lab-on-chip technologies frequently require biological samples, such as cells or microorganisms, to be maintained inside a syringe for prolonged periods of time during operations. Challenges include preventing cell sedimentation, ensuring cell viability, and maintaining buffer rheological properties...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | HardwareX |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468067225000161 |
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| author | Maryamsadat Ghoreishi Giovanna Peruzzi Lucia Iafrate Gianluca Cidonio Noemi D’Abbondanza Giancarlo Ruocco Marco Leonetti Riccardo Reale |
| author_facet | Maryamsadat Ghoreishi Giovanna Peruzzi Lucia Iafrate Gianluca Cidonio Noemi D’Abbondanza Giancarlo Ruocco Marco Leonetti Riccardo Reale |
| author_sort | Maryamsadat Ghoreishi |
| collection | DOAJ |
| description | Lab-on-chip technologies frequently require biological samples, such as cells or microorganisms, to be maintained inside a syringe for prolonged periods of time during operations. Challenges include preventing cell sedimentation, ensuring cell viability, and maintaining buffer rheological properties (i.e. viscosity and density) constant, particularly in applications like 3D bioprinting and diagnostic assays. To address these challenges, we have developed the Syringe Electromagnetic Controller (SEC), an integrated system capable of simultaneously stirring and thermoregulating samples inside a syringe. SEC prevents sedimentation through the cyclic movement of a magnet actuated by an electromagnetic field, while maintaining a stable temperature (within ± 0.5 °C from a set-point) with a feedback loop. The system is compact, cost-effective, and easily integrated into various setups. Experimental validation shows that SEC effectively keeps living cells in suspension and at a constant temperature without compromising cell viability. Thus, we have ultimately demonstrated the functionality of SEC as a versatile solution for enhancing the reliability of lab-on-chip applications. |
| format | Article |
| id | doaj-art-304b601c43ef4fdf82142e62333dbbfc |
| institution | DOAJ |
| issn | 2468-0672 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | HardwareX |
| spelling | doaj-art-304b601c43ef4fdf82142e62333dbbfc2025-08-20T03:11:26ZengElsevierHardwareX2468-06722025-06-0122e0063810.1016/j.ohx.2025.e00638A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticlesMaryamsadat Ghoreishi0Giovanna Peruzzi1Lucia Iafrate2Gianluca Cidonio3Noemi D’Abbondanza4Giancarlo Ruocco5Marco Leonetti6Riccardo Reale7Center for Life Nano- and Neuro- Sciences, Italian Institute of Technology, 00185 Rome, Italy; Department of Basic and Applied Science for Engineering, Sapienza University of Rome 00161 Rome, ItalyCenter for Life Nano- and Neuro- Sciences, Italian Institute of Technology, 00185 Rome, ItalyCenter for Life Nano- and Neuro- Sciences, Italian Institute of Technology, 00185 Rome, ItalyCenter for Life Nano- and Neuro- Sciences, Italian Institute of Technology, 00185 Rome, Italy; Department of Mechanical and Aerospace Engineering, Sapienza University of Rome 00184 Rome, ItalyCenter for Life Nano- and Neuro- Sciences, Italian Institute of Technology, 00185 Rome, Italy; International School of Advanced Studies, University of Camerino, Via Gentile III Da Varano, 62032 Camerino, ItalyCenter for Life Nano- and Neuro- Sciences, Italian Institute of Technology, 00185 Rome, Italy; Department of Physics, Sapienza University of Rome, Piazzale A. Moro 5, 00185 Rome, ItalyCenter for Life Nano- and Neuro- Sciences, Italian Institute of Technology, 00185 Rome, Italy; Institute of Nanotechnology of the National Research Council of Italy, CNR-NANOTEC, Rome Unit, Piazzale A. Moro 5, I-00185 Rome, ItalyCenter for Life Nano- and Neuro- Sciences, Italian Institute of Technology, 00185 Rome, Italy; Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Rome, Italy; Corresponding author at: Department of Civil Engineering and Computer Science, University of Rome Tor Vergata, Rome, ItalyLab-on-chip technologies frequently require biological samples, such as cells or microorganisms, to be maintained inside a syringe for prolonged periods of time during operations. Challenges include preventing cell sedimentation, ensuring cell viability, and maintaining buffer rheological properties (i.e. viscosity and density) constant, particularly in applications like 3D bioprinting and diagnostic assays. To address these challenges, we have developed the Syringe Electromagnetic Controller (SEC), an integrated system capable of simultaneously stirring and thermoregulating samples inside a syringe. SEC prevents sedimentation through the cyclic movement of a magnet actuated by an electromagnetic field, while maintaining a stable temperature (within ± 0.5 °C from a set-point) with a feedback loop. The system is compact, cost-effective, and easily integrated into various setups. Experimental validation shows that SEC effectively keeps living cells in suspension and at a constant temperature without compromising cell viability. Thus, we have ultimately demonstrated the functionality of SEC as a versatile solution for enhancing the reliability of lab-on-chip applications.http://www.sciencedirect.com/science/article/pii/S2468067225000161SedimentationStirringHeatingSyringe thermoregulation3D bioprinting |
| spellingShingle | Maryamsadat Ghoreishi Giovanna Peruzzi Lucia Iafrate Gianluca Cidonio Noemi D’Abbondanza Giancarlo Ruocco Marco Leonetti Riccardo Reale A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles HardwareX Sedimentation Stirring Heating Syringe thermoregulation 3D bioprinting |
| title | A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles |
| title_full | A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles |
| title_fullStr | A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles |
| title_full_unstemmed | A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles |
| title_short | A compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles |
| title_sort | compact electromagnetic syringe stirrer and temperature controller for the reliable dispensing of living cells and microparticles |
| topic | Sedimentation Stirring Heating Syringe thermoregulation 3D bioprinting |
| url | http://www.sciencedirect.com/science/article/pii/S2468067225000161 |
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