Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia
Aim. To investigate optochin-resistant viridans group streptococci (VGS) strains isolated from the sputum sample of adult patients with different clinical symptoms. Materials and Methods. Optochin-resistant VGS isolates were identified by matrix-assisted laser desorption ionization time of flight ma...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/6646925 |
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| author | Wisiva Tofriska Paramaiswari Nurma Sumar Sidik Miftahuddin Majid Khoeri Wisnu Tafroji Wahyu Finasari Said Dodi Safari |
| author_facet | Wisiva Tofriska Paramaiswari Nurma Sumar Sidik Miftahuddin Majid Khoeri Wisnu Tafroji Wahyu Finasari Said Dodi Safari |
| author_sort | Wisiva Tofriska Paramaiswari |
| collection | DOAJ |
| description | Aim. To investigate optochin-resistant viridans group streptococci (VGS) strains isolated from the sputum sample of adult patients with different clinical symptoms. Materials and Methods. Optochin-resistant VGS isolates were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). recA sequencing was used to confirm identified isolates at the genus level by MALDI-TOF MS. Finding. We identified 79% of tested isolates (148/187) at the species-level identification using the MALDI-TOF MS tool. We identified that the most common species isolated from sputum specimens were S. oralis (44.9%) followed by S. mitis (25.7%), S. infantis (9.1%), S. parasanguinis (7.5%), S. peroris (3.7%), S. anginosus (2.7%), and S. sanguinis (2.1%). Discussion. The S. oralis strains were majority of optochin-resistant VGS isolates obtained from sputum of adult patients in Jakarta, Indonesia. MALDI-TOF MS showed potential for the rapid identification tool to identify optochin-resistant VGS isolates. Although there were discrepancies in identifying isolates at the genus/species level, the performance could be improved by expanding its database. |
| format | Article |
| id | doaj-art-30495b8b51e642c1b9de90698011220e |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-30495b8b51e642c1b9de90698011220e2025-02-03T07:24:04ZengWileyInternational Journal of Microbiology1687-918X1687-91982021-01-01202110.1155/2021/66469256646925Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, IndonesiaWisiva Tofriska Paramaiswari0Nurma Sumar Sidik1Miftahuddin Majid Khoeri2Wisnu Tafroji3Wahyu Finasari Said4Dodi Safari5Eijkman Institute for Molecular Biology, Jakarta, IndonesiaDepartment of Biochemistry, Bogor Agricultural University, Bogor, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaDepartment of Clinical Pathology, Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo Hospital, Jakarta, IndonesiaEijkman Institute for Molecular Biology, Jakarta, IndonesiaAim. To investigate optochin-resistant viridans group streptococci (VGS) strains isolated from the sputum sample of adult patients with different clinical symptoms. Materials and Methods. Optochin-resistant VGS isolates were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). recA sequencing was used to confirm identified isolates at the genus level by MALDI-TOF MS. Finding. We identified 79% of tested isolates (148/187) at the species-level identification using the MALDI-TOF MS tool. We identified that the most common species isolated from sputum specimens were S. oralis (44.9%) followed by S. mitis (25.7%), S. infantis (9.1%), S. parasanguinis (7.5%), S. peroris (3.7%), S. anginosus (2.7%), and S. sanguinis (2.1%). Discussion. The S. oralis strains were majority of optochin-resistant VGS isolates obtained from sputum of adult patients in Jakarta, Indonesia. MALDI-TOF MS showed potential for the rapid identification tool to identify optochin-resistant VGS isolates. Although there were discrepancies in identifying isolates at the genus/species level, the performance could be improved by expanding its database.http://dx.doi.org/10.1155/2021/6646925 |
| spellingShingle | Wisiva Tofriska Paramaiswari Nurma Sumar Sidik Miftahuddin Majid Khoeri Wisnu Tafroji Wahyu Finasari Said Dodi Safari Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia International Journal of Microbiology |
| title | Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia |
| title_full | Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia |
| title_fullStr | Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia |
| title_full_unstemmed | Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia |
| title_short | Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia |
| title_sort | isolation and identification of optochin resistant viridans group streptococci from the sputum samples of adult patients in jakarta indonesia |
| url | http://dx.doi.org/10.1155/2021/6646925 |
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