Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea
Abundant biologically active peptides have been discovered from frog skin secretions, a rich natural source of bioactive compounds with great potential in drug discovery. In this study, three Caeridin peptides, namely, Caeridin-1, S5-Caeridin-1, and Caeridin-a1, were discovered from the skin secreti...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/8158453 |
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| author | Lei Li Qing Wu Xi Wang Huimin Lu Xinping Xi Mei Zhou Chris J. Watson Tianbao Chen Lei Wang |
| author_facet | Lei Li Qing Wu Xi Wang Huimin Lu Xinping Xi Mei Zhou Chris J. Watson Tianbao Chen Lei Wang |
| author_sort | Lei Li |
| collection | DOAJ |
| description | Abundant biologically active peptides have been discovered from frog skin secretions, a rich natural source of bioactive compounds with great potential in drug discovery. In this study, three Caeridin peptides, namely, Caeridin-1, S5-Caeridin-1, and Caeridin-a1, were discovered from the skin secretion of the Australian White’s tree frog, Litoria caerulea, for the first time, by means of combining transcriptomic and peptidomic analyses. It also represents the first report on bioactive Caeridins since this family of peptides was initially studied 20 years ago. Chemically synthetic versions of each natural Caeridin demonstrated promising bioactivities either on rat smooth muscles or against microbial growth. Specifically, Caeridin-1 produced contraction of rat bladder smooth muscle, while S5-Caeridin-1 induced relaxation of rat ileum smooth muscle, both at nanomolar concentrations. Moreover, Caeridin-a1 was shown to potently inhibit the growth of the planktonic Gram-positive bacteria Staphylococcus aureus (S. aureus), methicillin-resistant S. aureus (MRSA), and Enterococcus faecalis (E. faecalis), the Gram-negative bacterium, Escherichia coli (E. coli), and the yeast, Candida albicans (C. albicans). The discovery of these Caeridins may induce further intensive and systematic studies of frog skin peptides to promote the discovery of natural templates as lead compounds for drug discovery and therapeutic application. |
| format | Article |
| id | doaj-art-30444cbc5b664775a9ec349025f74a6e |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-30444cbc5b664775a9ec349025f74a6e2025-02-03T06:11:30ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/81584538158453Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caeruleaLei Li0Qing Wu1Xi Wang2Huimin Lu3Xinping Xi4Mei Zhou5Chris J. Watson6Tianbao Chen7Lei Wang8Natural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, UKSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaNatural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, UKNatural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, UKNatural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, UKNatural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, UKCentre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University, Belfast BT9 7BL, UKNatural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, UKNatural Drug Discovery Group, School of Pharmacy, Queen’s University, Belfast BT9 7BL, UKAbundant biologically active peptides have been discovered from frog skin secretions, a rich natural source of bioactive compounds with great potential in drug discovery. In this study, three Caeridin peptides, namely, Caeridin-1, S5-Caeridin-1, and Caeridin-a1, were discovered from the skin secretion of the Australian White’s tree frog, Litoria caerulea, for the first time, by means of combining transcriptomic and peptidomic analyses. It also represents the first report on bioactive Caeridins since this family of peptides was initially studied 20 years ago. Chemically synthetic versions of each natural Caeridin demonstrated promising bioactivities either on rat smooth muscles or against microbial growth. Specifically, Caeridin-1 produced contraction of rat bladder smooth muscle, while S5-Caeridin-1 induced relaxation of rat ileum smooth muscle, both at nanomolar concentrations. Moreover, Caeridin-a1 was shown to potently inhibit the growth of the planktonic Gram-positive bacteria Staphylococcus aureus (S. aureus), methicillin-resistant S. aureus (MRSA), and Enterococcus faecalis (E. faecalis), the Gram-negative bacterium, Escherichia coli (E. coli), and the yeast, Candida albicans (C. albicans). The discovery of these Caeridins may induce further intensive and systematic studies of frog skin peptides to promote the discovery of natural templates as lead compounds for drug discovery and therapeutic application.http://dx.doi.org/10.1155/2018/8158453 |
| spellingShingle | Lei Li Qing Wu Xi Wang Huimin Lu Xinping Xi Mei Zhou Chris J. Watson Tianbao Chen Lei Wang Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea International Journal of Genomics |
| title | Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea |
| title_full | Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea |
| title_fullStr | Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea |
| title_full_unstemmed | Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea |
| title_short | Discovery of Novel Caeridins from the Skin Secretion of the Australian White’s Tree Frog, Litoria caerulea |
| title_sort | discovery of novel caeridins from the skin secretion of the australian white s tree frog litoria caerulea |
| url | http://dx.doi.org/10.1155/2018/8158453 |
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