Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.)
The plant hormone ethylene elicits crucial regulatory effects on plant growth, development, and stress resistance. As the enzyme that catalyzes the final step of ethylene biosynthesis, 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) plays a key role in precisely controlling ethylene production....
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/13/24/3490 |
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| Summary: | The plant hormone ethylene elicits crucial regulatory effects on plant growth, development, and stress resistance. As the enzyme that catalyzes the final step of ethylene biosynthesis, 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) plays a key role in precisely controlling ethylene production. However, the functional characterization of the <i>ACO</i> gene family in rice remains largely unexplored. In this study, we performed a phylogenetic analysis of seven <i>OsACO</i> genes (<i>OsACO1</i>–<i>OsACO7</i>), which were classified into three subfamilies (Types I, II, and III). The members within the same clades exhibited similar tertiary structures and conserved protein motifs. We conducted inter/intraspecies covariance assays of OsACOs to elucidate their evolutionary and duplication events. Numerous cis-acting elements identified in <i>OsACO</i> promoter regions are associated with development, hormonal stimuli, and environmental responses. The expression assay by RT-qPCR revealed that <i>OsACO</i> genes exhibited tissue-specific expression and were significantly altered under various abiotic stresses, indicating their potential involvement in these processes regulated at the transcriptional level. Additionally, we predicted candidate-targeting miRNAs and identified putative cysteine sites of S-nitrosylation (SNO) and S-sulfhydration (SSH) in OsACOs, providing insights into their post-transcriptional and post-translational regulatory mechanisms. These findings pave the way for the further investigation of OsACO functions and their potential applications in improving rice growth and stress resilience by modulating ethylene biosynthesis. |
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| ISSN: | 2223-7747 |