Therapeutic potential of super activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) in enhancing endometrial regeneration in rats with thin endometrium
Abstract This study aimed to establish a rat model of thin endometrium and investigate the effects of super-activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) on the thin endometrium in rats. Thin endometrium models were induced by infusing absolute ethyl alcohol into...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-10587-w |
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| author | Ling-Qi Meng Yi Zhang Chun-Xiang Liu Ihsan Ullah Tian-Qi Zhang Yi Zhang |
| author_facet | Ling-Qi Meng Yi Zhang Chun-Xiang Liu Ihsan Ullah Tian-Qi Zhang Yi Zhang |
| author_sort | Ling-Qi Meng |
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| description | Abstract This study aimed to establish a rat model of thin endometrium and investigate the effects of super-activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) on the thin endometrium in rats. Thin endometrium models were induced by infusing absolute ethyl alcohol into the uteri of female Sprague-Dawley (SD) rats. Rats were randomly assigned to several groups (Control, Model, Extracellular matrix (ECM) + sPL, ECM + cell, Gel + sPL, Gel + cell) and treated for 21 or 42 days. Histopathological structures and endometrial thickness were observed using hematoxylin-eosin (HE) staining. ELISA was used to detect PDGF-BB, TGF-β1, E2 and FSH expression levels in serum. Furthermore, Western blot and immunohistochemical staining were used to assess the expression levels of cyclin D1, CD34, pan-keratin, cytokeratin 18, and vimentin in uterine tissue. HE staining revealed improvements in endometrial thickness, gland number, and blood vessels following treatment with sPL and UCMSCs in the thin endometrium rat model. Compared to the model group, ELISA results demonstrated that the PDGF-BB, E2, TGF-β1 and FSH serum in treatment groups returned to control levels. Immunohistochemical staining and Western blot results indicated decreased keratin, cytokeratin, and vimentin expression levels in the model group, which were significantly increased by sPL perfusion or UCMSCs transplantation. Intrauterine perfusion of sPL and UCMSCs improves endometrium thickness, morphology, function, and repair capacity in rats with thin endometrium. |
| format | Article |
| id | doaj-art-2ff69616d57d497c801fac9dfe77b1f0 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-2ff69616d57d497c801fac9dfe77b1f02025-08-20T03:46:04ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-10587-wTherapeutic potential of super activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) in enhancing endometrial regeneration in rats with thin endometriumLing-Qi Meng0Yi Zhang1Chun-Xiang Liu2Ihsan Ullah3Tian-Qi Zhang4Yi Zhang5National and Local Joint Stem Cell Research and Engineering Center for Aging Diseases, Tian Qing Stem Cell Co. LtdLaboratory of Basic and Application Research of Bei yao, Heilongjiang University of Chinese Medicine, Ministry of EducationNational and Local Joint Stem Cell Research and Engineering Center for Aging Diseases, Tian Qing Stem Cell Co. LtdNational and Local Joint Stem Cell Research and Engineering Center for Aging Diseases, Tian Qing Stem Cell Co. LtdNational and Local Joint Stem Cell Research and Engineering Center for Aging Diseases, Tian Qing Stem Cell Co. LtdNational and Local Joint Stem Cell Research and Engineering Center for Aging Diseases, Tian Qing Stem Cell Co. LtdAbstract This study aimed to establish a rat model of thin endometrium and investigate the effects of super-activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) on the thin endometrium in rats. Thin endometrium models were induced by infusing absolute ethyl alcohol into the uteri of female Sprague-Dawley (SD) rats. Rats were randomly assigned to several groups (Control, Model, Extracellular matrix (ECM) + sPL, ECM + cell, Gel + sPL, Gel + cell) and treated for 21 or 42 days. Histopathological structures and endometrial thickness were observed using hematoxylin-eosin (HE) staining. ELISA was used to detect PDGF-BB, TGF-β1, E2 and FSH expression levels in serum. Furthermore, Western blot and immunohistochemical staining were used to assess the expression levels of cyclin D1, CD34, pan-keratin, cytokeratin 18, and vimentin in uterine tissue. HE staining revealed improvements in endometrial thickness, gland number, and blood vessels following treatment with sPL and UCMSCs in the thin endometrium rat model. Compared to the model group, ELISA results demonstrated that the PDGF-BB, E2, TGF-β1 and FSH serum in treatment groups returned to control levels. Immunohistochemical staining and Western blot results indicated decreased keratin, cytokeratin, and vimentin expression levels in the model group, which were significantly increased by sPL perfusion or UCMSCs transplantation. Intrauterine perfusion of sPL and UCMSCs improves endometrium thickness, morphology, function, and repair capacity in rats with thin endometrium.https://doi.org/10.1038/s41598-025-10587-wThin endometriumSuper activated platelet lysate (sPL)Umbilical cord mesenchymal stem cells (UCMSCs)Endometrium regeneration |
| spellingShingle | Ling-Qi Meng Yi Zhang Chun-Xiang Liu Ihsan Ullah Tian-Qi Zhang Yi Zhang Therapeutic potential of super activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) in enhancing endometrial regeneration in rats with thin endometrium Scientific Reports Thin endometrium Super activated platelet lysate (sPL) Umbilical cord mesenchymal stem cells (UCMSCs) Endometrium regeneration |
| title | Therapeutic potential of super activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) in enhancing endometrial regeneration in rats with thin endometrium |
| title_full | Therapeutic potential of super activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) in enhancing endometrial regeneration in rats with thin endometrium |
| title_fullStr | Therapeutic potential of super activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) in enhancing endometrial regeneration in rats with thin endometrium |
| title_full_unstemmed | Therapeutic potential of super activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) in enhancing endometrial regeneration in rats with thin endometrium |
| title_short | Therapeutic potential of super activated platelet lysate (sPL) and umbilical cord mesenchymal stem cells (UCMSCs) in enhancing endometrial regeneration in rats with thin endometrium |
| title_sort | therapeutic potential of super activated platelet lysate spl and umbilical cord mesenchymal stem cells ucmscs in enhancing endometrial regeneration in rats with thin endometrium |
| topic | Thin endometrium Super activated platelet lysate (sPL) Umbilical cord mesenchymal stem cells (UCMSCs) Endometrium regeneration |
| url | https://doi.org/10.1038/s41598-025-10587-w |
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