A Canadian Isolate of Hepatitis D (delta) Virus
HepatitisD(delta) virus (HDV) is an infectious agent that propagates in hepatocytes only in the presence of hepatitis B virus, causing fulminant or chronic hepatitis with liver cirrhosis. HDV is a 36 nm particle that includes a circular RNA genome of 1.7 kilobases with an extensive internal compleme...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/2000/397947 |
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| _version_ | 1832556052180107264 |
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| author | Véronique Poisson Daniel B Ménard Éric Frost Jean-Pierre Perreault |
| author_facet | Véronique Poisson Daniel B Ménard Éric Frost Jean-Pierre Perreault |
| author_sort | Véronique Poisson |
| collection | DOAJ |
| description | HepatitisD(delta) virus (HDV) is an infectious
agent that propagates in hepatocytes only in the presence
of hepatitis B virus, causing fulminant or chronic hepatitis with
liver cirrhosis. HDV is a 36 nm particle that includes a circular
RNA genome of 1.7 kilobases with an extensive internal complementary
that allows it to fold into a rod-like structure. The relationships
among genotypes, sequence variability, geographical
distribution and disease severity of HDV remain unknown. Consequently,
in the present study, the complete nucleotide sequence
of anHDVisolated from a Canadian patient was determined. The
viral RNA from serum was amplified using reverse transcription
coupled to polymerase chain reaction amplification. The resulting
complementary DNA was cloned and sequenced. Sequence
analysis revealed that this new isolate contained 1672 nucleotides
corresponding to genotype 1, which has a worldwide distribution.
Sequencing of four independent clones revealed 17 substitutions,
corresponding to an overall sequence variability of 1%. Surprisingly,
seven mutations were found in the 48-nucleotide region located
between the two highly conserved self-catalytic motifs. This
is the first demonstration that many substitutions are identified in
this region of HDV, and prompts the present authors to define it
as a hypervariable region. |
| format | Article |
| id | doaj-art-2fdb763f9a6840169c7d6eaebb160d2c |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-2fdb763f9a6840169c7d6eaebb160d2c2025-02-03T05:46:22ZengWileyCanadian Journal of Gastroenterology0835-79002000-01-0114Suppl B36B41B10.1155/2000/397947A Canadian Isolate of Hepatitis D (delta) VirusVéronique Poisson0Daniel B Ménard1Éric Frost2Jean-Pierre Perreault31Département de Biochimie, Faculté de médecine, Université de Sherbrooke, Sherbrooke, Québec, CanadaDépartement de Médecine, Faculté de médecine, Université de Sherbrooke, Sherbrooke, Québec, CanadaDépartement de Microbiologie et Infectiologie, Faculté de médecine, Université de Sherbrooke, Sherbrooke, Québec, Canada1Département de Biochimie, Faculté de médecine, Université de Sherbrooke, Sherbrooke, Québec, CanadaHepatitisD(delta) virus (HDV) is an infectious agent that propagates in hepatocytes only in the presence of hepatitis B virus, causing fulminant or chronic hepatitis with liver cirrhosis. HDV is a 36 nm particle that includes a circular RNA genome of 1.7 kilobases with an extensive internal complementary that allows it to fold into a rod-like structure. The relationships among genotypes, sequence variability, geographical distribution and disease severity of HDV remain unknown. Consequently, in the present study, the complete nucleotide sequence of anHDVisolated from a Canadian patient was determined. The viral RNA from serum was amplified using reverse transcription coupled to polymerase chain reaction amplification. The resulting complementary DNA was cloned and sequenced. Sequence analysis revealed that this new isolate contained 1672 nucleotides corresponding to genotype 1, which has a worldwide distribution. Sequencing of four independent clones revealed 17 substitutions, corresponding to an overall sequence variability of 1%. Surprisingly, seven mutations were found in the 48-nucleotide region located between the two highly conserved self-catalytic motifs. This is the first demonstration that many substitutions are identified in this region of HDV, and prompts the present authors to define it as a hypervariable region.http://dx.doi.org/10.1155/2000/397947 |
| spellingShingle | Véronique Poisson Daniel B Ménard Éric Frost Jean-Pierre Perreault A Canadian Isolate of Hepatitis D (delta) Virus Canadian Journal of Gastroenterology |
| title | A Canadian Isolate of Hepatitis D (delta) Virus |
| title_full | A Canadian Isolate of Hepatitis D (delta) Virus |
| title_fullStr | A Canadian Isolate of Hepatitis D (delta) Virus |
| title_full_unstemmed | A Canadian Isolate of Hepatitis D (delta) Virus |
| title_short | A Canadian Isolate of Hepatitis D (delta) Virus |
| title_sort | canadian isolate of hepatitis d delta virus |
| url | http://dx.doi.org/10.1155/2000/397947 |
| work_keys_str_mv | AT veroniquepoisson acanadianisolateofhepatitisddeltavirus AT danielbmenard acanadianisolateofhepatitisddeltavirus AT ericfrost acanadianisolateofhepatitisddeltavirus AT jeanpierreperreault acanadianisolateofhepatitisddeltavirus AT veroniquepoisson canadianisolateofhepatitisddeltavirus AT danielbmenard canadianisolateofhepatitisddeltavirus AT ericfrost canadianisolateofhepatitisddeltavirus AT jeanpierreperreault canadianisolateofhepatitisddeltavirus |