Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose
Lactose is a widely used pharmaceutical excipient, sometimes irreplaceable. Traces of residual proteins left during production of lactose are potential allergen to body. The present paper describes a sensitive and specific LC-MS method for the determination of α-lactalbumin (α-La) in lactose samples...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/841084 |
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| author | Rui Yan Longmei Qu Nan Luo Yang Liu Yu Liu Li Li Lijiang Chen |
| author_facet | Rui Yan Longmei Qu Nan Luo Yang Liu Yu Liu Li Li Lijiang Chen |
| author_sort | Rui Yan |
| collection | DOAJ |
| description | Lactose is a widely used pharmaceutical excipient, sometimes irreplaceable. Traces of residual proteins left during production of lactose are potential allergen to body. The present paper describes a sensitive and specific LC-MS method for the determination of α-lactalbumin (α-La) in lactose samples. Chromatographic separation was performed on an Acquity UPLC BEH300 C18 column (2.1×150 mm, 1.7 μm) with an isocratic mobile phase consisting of water containing 0.1% TFA and acetonitrile containing 0.1% TFA (80 : 20, v/v). Mass spectrometric detection was achieved by a triple-quadrupole mass spectrometer equipped with an ESI interface operating in positive ionization mode. Quantitation was performed using selected ion monitoring of m/z 2364 for α-La. The calibration curve was linear from 0.2 to 10 µg/mL. The intra- and interday precisions were less than 7.6% and the accuracy ranged from 96.4 to 104.5%. The limit of quantification (LOQ) was 0.15 µg/mL and the limit of detection (LOD) was 0.05 µg/mL. This method was then successfully applied to investigate 6 different lactose samples. The application can provide technical preparation for the development of specification of lactose. |
| format | Article |
| id | doaj-art-2fd9b4a9b15545c6bffbe4cc0baa4f31 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-2fd9b4a9b15545c6bffbe4cc0baa4f312025-02-03T01:12:26ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792014-01-01201410.1155/2014/841084841084Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant LactoseRui Yan0Longmei Qu1Nan Luo2Yang Liu3Yu Liu4Li Li5Lijiang Chen6College of Pharmacy, Liaoning University, Shenyang 110036, ChinaYichang Humanwell Pharmaceutical Co., Ltd., Yichang 443005, ChinaYichang Humanwell Pharmaceutical Co., Ltd., Yichang 443005, ChinaCollege of Pharmacy, Liaoning University, Shenyang 110036, ChinaCollege of Pharmacy, Liaoning University, Shenyang 110036, ChinaCollege of Pharmacy, Liaoning University, Shenyang 110036, ChinaCollege of Pharmacy, Liaoning University, Shenyang 110036, ChinaLactose is a widely used pharmaceutical excipient, sometimes irreplaceable. Traces of residual proteins left during production of lactose are potential allergen to body. The present paper describes a sensitive and specific LC-MS method for the determination of α-lactalbumin (α-La) in lactose samples. Chromatographic separation was performed on an Acquity UPLC BEH300 C18 column (2.1×150 mm, 1.7 μm) with an isocratic mobile phase consisting of water containing 0.1% TFA and acetonitrile containing 0.1% TFA (80 : 20, v/v). Mass spectrometric detection was achieved by a triple-quadrupole mass spectrometer equipped with an ESI interface operating in positive ionization mode. Quantitation was performed using selected ion monitoring of m/z 2364 for α-La. The calibration curve was linear from 0.2 to 10 µg/mL. The intra- and interday precisions were less than 7.6% and the accuracy ranged from 96.4 to 104.5%. The limit of quantification (LOQ) was 0.15 µg/mL and the limit of detection (LOD) was 0.05 µg/mL. This method was then successfully applied to investigate 6 different lactose samples. The application can provide technical preparation for the development of specification of lactose.http://dx.doi.org/10.1155/2014/841084 |
| spellingShingle | Rui Yan Longmei Qu Nan Luo Yang Liu Yu Liu Li Li Lijiang Chen Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose International Journal of Analytical Chemistry |
| title | Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose |
| title_full | Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose |
| title_fullStr | Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose |
| title_full_unstemmed | Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose |
| title_short | Quantitation of α-Lactalbumin by Liquid Chromatography Tandem Mass Spectrometry in Medicinal Adjuvant Lactose |
| title_sort | quantitation of α lactalbumin by liquid chromatography tandem mass spectrometry in medicinal adjuvant lactose |
| url | http://dx.doi.org/10.1155/2014/841084 |
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