Determination of Natamycin in Turkish Yoghurt

This study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150 mm × 4.6 mm × 5 µm) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303...

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Main Authors: Dilek Bilgic Alkaya, Ozlem Karalomlu
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2016/8480963
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author Dilek Bilgic Alkaya
Ozlem Karalomlu
author_facet Dilek Bilgic Alkaya
Ozlem Karalomlu
author_sort Dilek Bilgic Alkaya
collection DOAJ
description This study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150 mm × 4.6 mm × 5 µm) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303 nm. Natamycin was spiked into handmade yoghurt samples and used for validation. The method has been fully validated according to ISO 9233-2, 2007 (IDF 140-2, 2007). It was successfully applied to determination of 28 different Turkish yoghurt products. Findings dealing with the presence of natamycin in cheese samples are presented.
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institution Kabale University
issn 1687-8760
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publishDate 2016-01-01
publisher Wiley
record_format Article
series International Journal of Analytical Chemistry
spelling doaj-art-2fd1062e6e8e4ebb932ff1a270e4f0b52025-02-03T05:48:16ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792016-01-01201610.1155/2016/84809638480963Determination of Natamycin in Turkish YoghurtDilek Bilgic Alkaya0Ozlem Karalomlu1Faculty of Pharmacy, Department of Analytical Chemistry, Marmara University, P.O. Box 34668, Haydarpasa, Istanbul, TurkeyFaculty of Pharmacy, Department of Analytical Chemistry, Marmara University, P.O. Box 34668, Haydarpasa, Istanbul, TurkeyThis study was aimed at developing RP-HPLC method for determination of natamycin in Turkish yoghurt. Chromatographic separation was achieved on a C8 column (150 mm × 4.6 mm × 5 µm) with a mobile phase of methanol : water : acetic acid (12 : 8 : 1 v/v/v), at 1 mL/min flow rate with a detection of 303 nm. Natamycin was spiked into handmade yoghurt samples and used for validation. The method has been fully validated according to ISO 9233-2, 2007 (IDF 140-2, 2007). It was successfully applied to determination of 28 different Turkish yoghurt products. Findings dealing with the presence of natamycin in cheese samples are presented.http://dx.doi.org/10.1155/2016/8480963
spellingShingle Dilek Bilgic Alkaya
Ozlem Karalomlu
Determination of Natamycin in Turkish Yoghurt
International Journal of Analytical Chemistry
title Determination of Natamycin in Turkish Yoghurt
title_full Determination of Natamycin in Turkish Yoghurt
title_fullStr Determination of Natamycin in Turkish Yoghurt
title_full_unstemmed Determination of Natamycin in Turkish Yoghurt
title_short Determination of Natamycin in Turkish Yoghurt
title_sort determination of natamycin in turkish yoghurt
url http://dx.doi.org/10.1155/2016/8480963
work_keys_str_mv AT dilekbilgicalkaya determinationofnatamycininturkishyoghurt
AT ozlemkaralomlu determinationofnatamycininturkishyoghurt