Characterization, cytotoxicity and cell proliferation of two Hydroxyapatite products from Lates calcarifer fish bones via thermal calcination
This study investigates the sustainable extraction of nano-Hydroxyapatite (nano-HAp) from Lates calcarifer fish bones, a by-product of the seafood industry, through thermal calcination. Nano-HAp was synthesized at two temperatures (650 °C and 700 °C), resulting in rod-like (42 nm) and spherical (98...
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IOP Publishing
2025-01-01
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| Series: | Materials Research Express |
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| Online Access: | https://doi.org/10.1088/2053-1591/adb37f |
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| author | Khanh Hy Le Ho Viet Ha Dao Xuan Ky Pham Phuong Anh Nguyen Bao Vy Phan Thi Thiet Doan Xuan Vinh Tran |
| author_facet | Khanh Hy Le Ho Viet Ha Dao Xuan Ky Pham Phuong Anh Nguyen Bao Vy Phan Thi Thiet Doan Xuan Vinh Tran |
| author_sort | Khanh Hy Le Ho |
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| description | This study investigates the sustainable extraction of nano-Hydroxyapatite (nano-HAp) from Lates calcarifer fish bones, a by-product of the seafood industry, through thermal calcination. Nano-HAp was synthesized at two temperatures (650 °C and 700 °C), resulting in rod-like (42 nm) and spherical (98 nm) particles, respectively. Both samples were identified as B-carbonated HAp, with Ca/P molar ratios of 1.842 for HAp 650-4 h and 1.856 for HAp 700-4 h, confirming the presence of B-type HAp, which has higher Ca/P ratios than the standard HAp found in bones and teeth (1.67). Cytotoxicity tests showed that both samples exceeded the ISO 10993-5:2009 standard, with L929 cell viability above 70% at concentrations up to 2 mg mL ^−1 . Notably, the study emphasizes that the size and morphology of nano-HAp play a critical role in its biological activity. Larger spherical particles (HAp 700-4 h) enhanced the proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) more effectively than smaller rod-like particles (HAp 650-4 h), suggesting their greater potential for tissue engineering and bone regeneration applications. This work demonstrates the promising biocompatibility of fish bone-derived nano-HAp and its potential for sustainable biomedical applications. |
| format | Article |
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| institution | OA Journals |
| issn | 2053-1591 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-2fc8ba79525c4e008d13fde112f86f8e2025-08-20T02:13:23ZengIOP PublishingMaterials Research Express2053-15912025-01-0112202540210.1088/2053-1591/adb37fCharacterization, cytotoxicity and cell proliferation of two Hydroxyapatite products from Lates calcarifer fish bones via thermal calcinationKhanh Hy Le Ho0https://orcid.org/0000-0002-7635-2155Viet Ha Dao1Xuan Ky Pham2https://orcid.org/0000-0001-6767-7962Phuong Anh Nguyen3https://orcid.org/0009-0004-0616-1371Bao Vy Phan4https://orcid.org/0009-0001-6768-6313Thi Thiet Doan5https://orcid.org/0009-0005-6135-4288Xuan Vinh Tran6Institute of Oceanography, Vietnam Academy of Science and Technology (VAST), 01 Cau Da St., Nha Trang City 650000, Viet NamInstitute of Oceanography, Vietnam Academy of Science and Technology (VAST), 01 Cau Da St., Nha Trang City 650000, Viet NamInstitute of Oceanography, Vietnam Academy of Science and Technology (VAST), 01 Cau Da St., Nha Trang City 650000, Viet NamInstitute of Oceanography, Vietnam Academy of Science and Technology (VAST), 01 Cau Da St., Nha Trang City 650000, Viet NamInstitute of Oceanography, Vietnam Academy of Science and Technology (VAST), 01 Cau Da St., Nha Trang City 650000, Viet NamInstitute of Oceanography, Vietnam Academy of Science and Technology (VAST), 01 Cau Da St., Nha Trang City 650000, Viet NamFaculty of Odonto-Stomatology, University of Medicine and Pharmacy , 217 Hong Bang, Ho Chi Minh City 700000, Viet NamThis study investigates the sustainable extraction of nano-Hydroxyapatite (nano-HAp) from Lates calcarifer fish bones, a by-product of the seafood industry, through thermal calcination. Nano-HAp was synthesized at two temperatures (650 °C and 700 °C), resulting in rod-like (42 nm) and spherical (98 nm) particles, respectively. Both samples were identified as B-carbonated HAp, with Ca/P molar ratios of 1.842 for HAp 650-4 h and 1.856 for HAp 700-4 h, confirming the presence of B-type HAp, which has higher Ca/P ratios than the standard HAp found in bones and teeth (1.67). Cytotoxicity tests showed that both samples exceeded the ISO 10993-5:2009 standard, with L929 cell viability above 70% at concentrations up to 2 mg mL ^−1 . Notably, the study emphasizes that the size and morphology of nano-HAp play a critical role in its biological activity. Larger spherical particles (HAp 700-4 h) enhanced the proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) more effectively than smaller rod-like particles (HAp 650-4 h), suggesting their greater potential for tissue engineering and bone regeneration applications. This work demonstrates the promising biocompatibility of fish bone-derived nano-HAp and its potential for sustainable biomedical applications.https://doi.org/10.1088/2053-1591/adb37fcell proliferationlates calcarifer fish bonesmorphologyMTT assaynano-HApsize |
| spellingShingle | Khanh Hy Le Ho Viet Ha Dao Xuan Ky Pham Phuong Anh Nguyen Bao Vy Phan Thi Thiet Doan Xuan Vinh Tran Characterization, cytotoxicity and cell proliferation of two Hydroxyapatite products from Lates calcarifer fish bones via thermal calcination Materials Research Express cell proliferation lates calcarifer fish bones morphology MTT assay nano-HAp size |
| title | Characterization, cytotoxicity and cell proliferation of two Hydroxyapatite products from Lates calcarifer fish bones via thermal calcination |
| title_full | Characterization, cytotoxicity and cell proliferation of two Hydroxyapatite products from Lates calcarifer fish bones via thermal calcination |
| title_fullStr | Characterization, cytotoxicity and cell proliferation of two Hydroxyapatite products from Lates calcarifer fish bones via thermal calcination |
| title_full_unstemmed | Characterization, cytotoxicity and cell proliferation of two Hydroxyapatite products from Lates calcarifer fish bones via thermal calcination |
| title_short | Characterization, cytotoxicity and cell proliferation of two Hydroxyapatite products from Lates calcarifer fish bones via thermal calcination |
| title_sort | characterization cytotoxicity and cell proliferation of two hydroxyapatite products from lates calcarifer fish bones via thermal calcination |
| topic | cell proliferation lates calcarifer fish bones morphology MTT assay nano-HAp size |
| url | https://doi.org/10.1088/2053-1591/adb37f |
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