Molecular detection of Enterobacter hormaechei in bovine respiratory disease
Bovine respiratory disease (BRD) develops from complex interactions among environmental, host and pathogenic factors. This study aimed to phenotypically identify Enterobacter hormaechei isolated from cattle with BRD and assess antimicrobial susceptibility and determining the molecular phylogeny of l...
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Czech Academy of Agricultural Sciences
2024-12-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202412-0002_molecular-detection-of-enterobacter-hormaechei-in-bovine-respiratory-disease.php |
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| author | HAJ Gharban |
| author_facet | HAJ Gharban |
| author_sort | HAJ Gharban |
| collection | DOAJ |
| description | Bovine respiratory disease (BRD) develops from complex interactions among environmental, host and pathogenic factors. This study aimed to phenotypically identify Enterobacter hormaechei isolated from cattle with BRD and assess antimicrobial susceptibility and determining the molecular phylogeny of local E. hormaechei strains. Between November 2023 and March 2024, nasal swabs were collected from 93 cattle with BRD, before culturing for phenotypic analysis, and performing the polymerase chain reaction (PCR) for molecular characterisation. Of the 93 samples evaluated, 15.79% and 24.56% tested positive for E. hormaechei isolates on culture and PCR, respectively. The local isolates exhibited high resistance to amoxicillin, ampicillin, amikacin, nalidixic acid and ceftazidime; high susceptibility to azithromycin, levofloxacin, gentamicin, ofloxacin, cefepime, ceftriaxone, cefotaxime, nitrofurantoin, ceftazidime and ciprofloxacin; and moderate susceptibility to ciprofloxacin, colistin, imipenem and meropenem. Multiple sequence alignment, phylogenetic tree analysis and homology sequence identification, showed that the five positive isolates were similar to the reference isolate. To the best of our knowledge, this is the first time that E. hormaechei has been isolated in cattle with BRD in Iraq. Because phenotype-based assays show limited accuracy to identify species, we recommend molecular and phylogenetic analysis be included in all similar studies in the future. |
| format | Article |
| id | doaj-art-2fb11260253c4fd5bcae4109103c24c4 |
| institution | DOAJ |
| issn | 0375-8427 1805-9392 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Veterinární Medicína |
| spelling | doaj-art-2fb11260253c4fd5bcae4109103c24c42025-08-20T02:52:52ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922024-12-01691240341210.17221/54/2024-VETMEDvet-202412-0002Molecular detection of Enterobacter hormaechei in bovine respiratory diseaseHAJ Gharban0Department of Internal and Preventive Veterinary Medicine, College of Veterinary Medicine, University of Wasit, Wasit, IraqBovine respiratory disease (BRD) develops from complex interactions among environmental, host and pathogenic factors. This study aimed to phenotypically identify Enterobacter hormaechei isolated from cattle with BRD and assess antimicrobial susceptibility and determining the molecular phylogeny of local E. hormaechei strains. Between November 2023 and March 2024, nasal swabs were collected from 93 cattle with BRD, before culturing for phenotypic analysis, and performing the polymerase chain reaction (PCR) for molecular characterisation. Of the 93 samples evaluated, 15.79% and 24.56% tested positive for E. hormaechei isolates on culture and PCR, respectively. The local isolates exhibited high resistance to amoxicillin, ampicillin, amikacin, nalidixic acid and ceftazidime; high susceptibility to azithromycin, levofloxacin, gentamicin, ofloxacin, cefepime, ceftriaxone, cefotaxime, nitrofurantoin, ceftazidime and ciprofloxacin; and moderate susceptibility to ciprofloxacin, colistin, imipenem and meropenem. Multiple sequence alignment, phylogenetic tree analysis and homology sequence identification, showed that the five positive isolates were similar to the reference isolate. To the best of our knowledge, this is the first time that E. hormaechei has been isolated in cattle with BRD in Iraq. Because phenotype-based assays show limited accuracy to identify species, we recommend molecular and phylogenetic analysis be included in all similar studies in the future.https://vetmed.agriculturejournals.cz/artkey/vet-202412-0002_molecular-detection-of-enterobacter-hormaechei-in-bovine-respiratory-disease.phpantibiotic sensitivity testenterobacter cloacae complexiraqphenotypic examinationphylogenetic analysisundifferentiated fever |
| spellingShingle | HAJ Gharban Molecular detection of Enterobacter hormaechei in bovine respiratory disease Veterinární Medicína antibiotic sensitivity test enterobacter cloacae complex iraq phenotypic examination phylogenetic analysis undifferentiated fever |
| title | Molecular detection of Enterobacter hormaechei in bovine respiratory disease |
| title_full | Molecular detection of Enterobacter hormaechei in bovine respiratory disease |
| title_fullStr | Molecular detection of Enterobacter hormaechei in bovine respiratory disease |
| title_full_unstemmed | Molecular detection of Enterobacter hormaechei in bovine respiratory disease |
| title_short | Molecular detection of Enterobacter hormaechei in bovine respiratory disease |
| title_sort | molecular detection of enterobacter hormaechei in bovine respiratory disease |
| topic | antibiotic sensitivity test enterobacter cloacae complex iraq phenotypic examination phylogenetic analysis undifferentiated fever |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202412-0002_molecular-detection-of-enterobacter-hormaechei-in-bovine-respiratory-disease.php |
| work_keys_str_mv | AT hajgharban moleculardetectionofenterobacterhormaecheiinbovinerespiratorydisease |