The kinetics of uracil-N-glycosylase distribution inside replication foci
Abstract Mismatched nucleobase uracil is commonly repaired through the base excision repair initiated by DNA uracil glycosylases. The data presented in this study strongly indicate that the nuclear uracil-N-glycosylase activity and nuclear protein content in human cell lines is highest in the S phas...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-84408-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585834801397760 |
|---|---|
| author | Anna Ligasová Ivo Frydrych Barbora Piskláková David Friedecký Karel Koberna |
| author_facet | Anna Ligasová Ivo Frydrych Barbora Piskláková David Friedecký Karel Koberna |
| author_sort | Anna Ligasová |
| collection | DOAJ |
| description | Abstract Mismatched nucleobase uracil is commonly repaired through the base excision repair initiated by DNA uracil glycosylases. The data presented in this study strongly indicate that the nuclear uracil-N-glycosylase activity and nuclear protein content in human cell lines is highest in the S phase of the cell cycle and that its distribution kinetics partially reflect the DNA replication activity in replication foci. In this respect, the data demonstrate structural changes of the replication focus related to the uracil-N-glycosylase distribution several dozens of minutes before end of its replication. The analysis also showed that very popular synchronisation protocols based on the double thymidine block can result in changes in the UNG2 content and uracil excision rate. In response, we propose a new method for the description of the changes of the content and the activity of different cell components during cell cycle without the necessity to use synchronisation protocols. |
| format | Article |
| id | doaj-art-2f6c5ee363f341f0bfd63a76ecedd280 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2f6c5ee363f341f0bfd63a76ecedd2802025-01-26T12:25:18ZengNature PortfolioScientific Reports2045-23222025-01-0115111910.1038/s41598-024-84408-xThe kinetics of uracil-N-glycosylase distribution inside replication fociAnna Ligasová0Ivo Frydrych1Barbora Piskláková2David Friedecký3Karel Koberna4Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University OlomoucInstitute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University OlomoucInstitute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University OlomoucLaboratory of Inherited Metabolic Disorders, Department of Clinical Chemistry, Palacký University and University Hospital OlomoucInstitute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacký University OlomoucAbstract Mismatched nucleobase uracil is commonly repaired through the base excision repair initiated by DNA uracil glycosylases. The data presented in this study strongly indicate that the nuclear uracil-N-glycosylase activity and nuclear protein content in human cell lines is highest in the S phase of the cell cycle and that its distribution kinetics partially reflect the DNA replication activity in replication foci. In this respect, the data demonstrate structural changes of the replication focus related to the uracil-N-glycosylase distribution several dozens of minutes before end of its replication. The analysis also showed that very popular synchronisation protocols based on the double thymidine block can result in changes in the UNG2 content and uracil excision rate. In response, we propose a new method for the description of the changes of the content and the activity of different cell components during cell cycle without the necessity to use synchronisation protocols.https://doi.org/10.1038/s41598-024-84408-x |
| spellingShingle | Anna Ligasová Ivo Frydrych Barbora Piskláková David Friedecký Karel Koberna The kinetics of uracil-N-glycosylase distribution inside replication foci Scientific Reports |
| title | The kinetics of uracil-N-glycosylase distribution inside replication foci |
| title_full | The kinetics of uracil-N-glycosylase distribution inside replication foci |
| title_fullStr | The kinetics of uracil-N-glycosylase distribution inside replication foci |
| title_full_unstemmed | The kinetics of uracil-N-glycosylase distribution inside replication foci |
| title_short | The kinetics of uracil-N-glycosylase distribution inside replication foci |
| title_sort | kinetics of uracil n glycosylase distribution inside replication foci |
| url | https://doi.org/10.1038/s41598-024-84408-x |
| work_keys_str_mv | AT annaligasova thekineticsofuracilnglycosylasedistributioninsidereplicationfoci AT ivofrydrych thekineticsofuracilnglycosylasedistributioninsidereplicationfoci AT barborapisklakova thekineticsofuracilnglycosylasedistributioninsidereplicationfoci AT davidfriedecky thekineticsofuracilnglycosylasedistributioninsidereplicationfoci AT karelkoberna thekineticsofuracilnglycosylasedistributioninsidereplicationfoci AT annaligasova kineticsofuracilnglycosylasedistributioninsidereplicationfoci AT ivofrydrych kineticsofuracilnglycosylasedistributioninsidereplicationfoci AT barborapisklakova kineticsofuracilnglycosylasedistributioninsidereplicationfoci AT davidfriedecky kineticsofuracilnglycosylasedistributioninsidereplicationfoci AT karelkoberna kineticsofuracilnglycosylasedistributioninsidereplicationfoci |