Leveraging CRISPR Cas9 RNPs and Cre-loxP in Picochlorum celeri for generation of field deployable strains and selection marker recycling

Leveraging CRISPR Cas9 RNPs and Cre-loxP in Picochlorum celeri for generation of field deployable strains and selection marker recycling

As new highly productive strains of algae are discovered and developed to meet the energy, chemical, and food requirements of the future, genetic engineering of those strains in a manner that yields deployable transformants is paramount. This study introduces the novel CRoxP (Cas9 RNPs coupled with...

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Bibliographic Details
Main Authors: Tyson A. Burch, Anagha Krishnan, Matthew C. Posewitz
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Microbiology
Subjects:
picochlorum
Cas9
Cre-LoxP
CRoxP
algae
LHC
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1588625/full
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