Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats
Consumption of high-fructose corn syrup (HFCS) in the diet is a causal factor in the development of abdominal obesity; however, the molecular mechanism behind this association is still up for debate. This study evaluated the metabolic disturbances that are caused by HFCS on adipose tissue as well as...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2023-05-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Kefir-Prevents-Adipose-Tissue-Growth-Through-the-Induction-of-Apoptotic-Elements,162710,0,2.html |
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| author | Esra Aslan Gokhan Sadi Hilal Guzel Cigdem Karaca Omer Adil Korkmaz Mine Kanat Pektas Mehmet Celegen Tugce Aladag Seyma Oncu Mehmet Bilgehan Pektas |
| author_facet | Esra Aslan Gokhan Sadi Hilal Guzel Cigdem Karaca Omer Adil Korkmaz Mine Kanat Pektas Mehmet Celegen Tugce Aladag Seyma Oncu Mehmet Bilgehan Pektas |
| author_sort | Esra Aslan |
| collection | DOAJ |
| description | Consumption of high-fructose corn syrup (HFCS) in the diet is a causal factor in the development of abdominal obesity; however, the molecular mechanism behind this association is still up for debate. This study evaluated the metabolic disturbances that are caused by HFCS on adipose tissue as well as the possibility of kefir as a therapy to prevent these metabolic disturbances. Male Wistar rats were divided into four groups: control, kefir, HFCS, and HFCS+kefir. HFCS (20%, w/v ) was given in drinking water and kefir (1 mL/100 g body weight) by gastric gavage daily for 8 weeks. Levels of insulin signaling, inflammation, and apoptosis-associated proteins of adipose tissues were determined with Western blot and immunohistochemical techniques. Gene expressions were evaluated with semi-quantitative real-time polymerase chain reaction (qRT-PCR). The indirect terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) method was used to assess changes in apoptotic cells, and hematoxylin/eosin staining to determine adipocyte number and diameter. Accordingly, HFCS boosted protein kinase B (Akt) and p-Akt while reducing nuclear factor κB (NF-κB), and tumor necrosis factor alpha (TNFα) levels and kefir treatment restored Akt induction in HFCS-treated rats despite raising NF-κB, and TNFα. Increased expression of Akt and B-cell lymphoma-2 gene ( Bcl2 ) was contrasted with decreased expression of Nfkb, Tnfa , tumor protein 53 gene ( p53 ), and caspase-8 gene ( Casp8 ). Furthermore, while there was a marked reduction in TUNEL-positive cells in the HFCS group, the number of such cells was greater in the HFCS+kefir group. These results show that HFCS intake suppresses apoptosis in adipose tissues, which may be responsible for tissue development and abdominal obesity and may be reversed with kefir administration due to the activation of apoptosis-associated genes and proteins. |
| format | Article |
| id | doaj-art-2f53c5a20b854e4eb4ff4356589bac96 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
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| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-2f53c5a20b854e4eb4ff4356589bac962025-02-03T06:48:52ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072023-05-0173212012910.31883/pjfns/162710162710Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed RatsEsra Aslan0https://orcid.org/0000-0002-3191-4978Gokhan Sadi1https://orcid.org/0000-0002-6422-1203Hilal Guzel2https://orcid.org/0000-0001-7692-8890Cigdem Karaca3https://orcid.org/0000-0003-2106-2422Omer Adil Korkmaz4https://orcid.org/0000-0001-5670-1314Mine Kanat Pektas5https://orcid.org/0000-0003-2862-3288Mehmet Celegen6https://orcid.org/0000-0002-6841-3675Tugce Aladag7https://orcid.org/0000-0003-3250-6113Seyma Oncu8https://orcid.org/0000-0003-2468-2416Mehmet Bilgehan Pektas9https://orcid.org/0000-0003-0055-7688Department of Histology and Embryology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, TurkeyDepartment of Biology, K.Ö. Science Faculty, Karamanoglu Mehmetbey University, 70100, Karaman, TurkeyDepartment of Anatomy, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, TurkeyDepartment of Chemistry, Faculty of Science, Yildiz Technical University, 34220, Istanbul, TurkeyDepartment of Obstetrics and Gynecology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, TurkeyDepartment of Pediatrics, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, TurkeyDepartment of Medical Pharmacology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, TurkeyDepartment of Medical Pharmacology, Faculty of Medicine, Afyonkarahisar Health Sciences University, 03200, Afyonkarahisar, TurkeyConsumption of high-fructose corn syrup (HFCS) in the diet is a causal factor in the development of abdominal obesity; however, the molecular mechanism behind this association is still up for debate. This study evaluated the metabolic disturbances that are caused by HFCS on adipose tissue as well as the possibility of kefir as a therapy to prevent these metabolic disturbances. Male Wistar rats were divided into four groups: control, kefir, HFCS, and HFCS+kefir. HFCS (20%, w/v ) was given in drinking water and kefir (1 mL/100 g body weight) by gastric gavage daily for 8 weeks. Levels of insulin signaling, inflammation, and apoptosis-associated proteins of adipose tissues were determined with Western blot and immunohistochemical techniques. Gene expressions were evaluated with semi-quantitative real-time polymerase chain reaction (qRT-PCR). The indirect terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) method was used to assess changes in apoptotic cells, and hematoxylin/eosin staining to determine adipocyte number and diameter. Accordingly, HFCS boosted protein kinase B (Akt) and p-Akt while reducing nuclear factor κB (NF-κB), and tumor necrosis factor alpha (TNFα) levels and kefir treatment restored Akt induction in HFCS-treated rats despite raising NF-κB, and TNFα. Increased expression of Akt and B-cell lymphoma-2 gene ( Bcl2 ) was contrasted with decreased expression of Nfkb, Tnfa , tumor protein 53 gene ( p53 ), and caspase-8 gene ( Casp8 ). Furthermore, while there was a marked reduction in TUNEL-positive cells in the HFCS group, the number of such cells was greater in the HFCS+kefir group. These results show that HFCS intake suppresses apoptosis in adipose tissues, which may be responsible for tissue development and abdominal obesity and may be reversed with kefir administration due to the activation of apoptosis-associated genes and proteins.http://journal.pan.olsztyn.pl/Kefir-Prevents-Adipose-Tissue-Growth-Through-the-Induction-of-Apoptotic-Elements,162710,0,2.htmladipose tissuefructosekefirapoptosisinflammation |
| spellingShingle | Esra Aslan Gokhan Sadi Hilal Guzel Cigdem Karaca Omer Adil Korkmaz Mine Kanat Pektas Mehmet Celegen Tugce Aladag Seyma Oncu Mehmet Bilgehan Pektas Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats Polish Journal of Food and Nutrition Sciences adipose tissue fructose kefir apoptosis inflammation |
| title | Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats |
| title_full | Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats |
| title_fullStr | Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats |
| title_full_unstemmed | Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats |
| title_short | Kefir Prevents Adipose Tissue Growth Through the Induction of Apoptotic Elements in High-Fructose Corn Syrup-Fed Rats |
| title_sort | kefir prevents adipose tissue growth through the induction of apoptotic elements in high fructose corn syrup fed rats |
| topic | adipose tissue fructose kefir apoptosis inflammation |
| url | http://journal.pan.olsztyn.pl/Kefir-Prevents-Adipose-Tissue-Growth-Through-the-Induction-of-Apoptotic-Elements,162710,0,2.html |
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