Copy number variations in AMY2B gene and amylase activity in Balkan dog breeds.
This study aims to estimate the number of AMY2B gene copies and measure serum amylase activity in several Balkan dog breeds. Additionally, it explores the relationship between these genetic and biochemical parameters. Blood samples from 85 dogs representing eight breeds were collected, DNA was extra...
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0322775 |
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| author | Jasmin Katica Teufik Goletić Aida Kavazović Maja Varatanović Ćazim Crnkić |
| author_facet | Jasmin Katica Teufik Goletić Aida Kavazović Maja Varatanović Ćazim Crnkić |
| author_sort | Jasmin Katica |
| collection | DOAJ |
| description | This study aims to estimate the number of AMY2B gene copies and measure serum amylase activity in several Balkan dog breeds. Additionally, it explores the relationship between these genetic and biochemical parameters. Blood samples from 85 dogs representing eight breeds were collected, DNA was extracted, and AMY2B copy numbers were determined using droplet digital PCR. AMY2B gene copies ranged from 7.7 to 18.4, with a mean of 12.4 ± 2.2. Significant breed-related differences were observed (p = 0.025), with Istrian Wire-Haired Hounds showing the highest mean copy number (13.9 ± 1.5) and Posavatz Hounds the lowest (10.8 ± 1.5). Serum amylase activity ranged from 3.3 to 17.8 µkat/L, with a mean of 8.7 ± 2.6, and showed significant interbreed differences (p = 0.004), with Barak breed displaying the highest activity. Serum glucose levels varied widely, but no significant interbreed differences were detected (p = 0.340). No significant correlation was found between AMY2B copy numbers and serum amylase activity or glucose levels. The study concludes that Balkan dogs have AMY2B copy numbers similar to other European breeds, likely reflecting historical agricultural practices in the region, thereby facilitating better starch digestion. While significant variations exist among breeds, the lack of correlation between gene copy number and amylase activity suggests that other factors influence enzyme levels. |
| format | Article |
| id | doaj-art-2f4bd0fdceb3438f85192daf0a2e7b10 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj-art-2f4bd0fdceb3438f85192daf0a2e7b102025-08-20T02:15:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01205e032277510.1371/journal.pone.0322775Copy number variations in AMY2B gene and amylase activity in Balkan dog breeds.Jasmin KaticaTeufik GoletićAida KavazovićMaja VaratanovićĆazim CrnkićThis study aims to estimate the number of AMY2B gene copies and measure serum amylase activity in several Balkan dog breeds. Additionally, it explores the relationship between these genetic and biochemical parameters. Blood samples from 85 dogs representing eight breeds were collected, DNA was extracted, and AMY2B copy numbers were determined using droplet digital PCR. AMY2B gene copies ranged from 7.7 to 18.4, with a mean of 12.4 ± 2.2. Significant breed-related differences were observed (p = 0.025), with Istrian Wire-Haired Hounds showing the highest mean copy number (13.9 ± 1.5) and Posavatz Hounds the lowest (10.8 ± 1.5). Serum amylase activity ranged from 3.3 to 17.8 µkat/L, with a mean of 8.7 ± 2.6, and showed significant interbreed differences (p = 0.004), with Barak breed displaying the highest activity. Serum glucose levels varied widely, but no significant interbreed differences were detected (p = 0.340). No significant correlation was found between AMY2B copy numbers and serum amylase activity or glucose levels. The study concludes that Balkan dogs have AMY2B copy numbers similar to other European breeds, likely reflecting historical agricultural practices in the region, thereby facilitating better starch digestion. While significant variations exist among breeds, the lack of correlation between gene copy number and amylase activity suggests that other factors influence enzyme levels.https://doi.org/10.1371/journal.pone.0322775 |
| spellingShingle | Jasmin Katica Teufik Goletić Aida Kavazović Maja Varatanović Ćazim Crnkić Copy number variations in AMY2B gene and amylase activity in Balkan dog breeds. PLoS ONE |
| title | Copy number variations in AMY2B gene and amylase activity in Balkan dog breeds. |
| title_full | Copy number variations in AMY2B gene and amylase activity in Balkan dog breeds. |
| title_fullStr | Copy number variations in AMY2B gene and amylase activity in Balkan dog breeds. |
| title_full_unstemmed | Copy number variations in AMY2B gene and amylase activity in Balkan dog breeds. |
| title_short | Copy number variations in AMY2B gene and amylase activity in Balkan dog breeds. |
| title_sort | copy number variations in amy2b gene and amylase activity in balkan dog breeds |
| url | https://doi.org/10.1371/journal.pone.0322775 |
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