A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application
An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B1, B2, B5, B6, B9, B12) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradie...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/359721 |
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| author | Margherita Grotzkyj Giorgi Kevin Howland Colin Martin Adrian B. Bonner |
| author_facet | Margherita Grotzkyj Giorgi Kevin Howland Colin Martin Adrian B. Bonner |
| author_sort | Margherita Grotzkyj Giorgi |
| collection | DOAJ |
| description | An HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B1, B2, B5, B6, B9, B12) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol. Total run time was 35 minutes. Detection was performed with diode array set at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples (24 plasma and urine samples from abstinent alcohol-dependent males). Interday and intraday precision were <4% and <7%, respectively, for all vitamins. Recovery percentages ranged from 93% to 100%. |
| format | Article |
| id | doaj-art-2f44548efda842fcb0dc9ee9b4ec0bbe |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-2f44548efda842fcb0dc9ee9b4ec0bbe2025-02-03T01:28:45ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/359721359721A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and ApplicationMargherita Grotzkyj Giorgi0Kevin Howland1Colin Martin2Adrian B. Bonner3Centre for Health Services Studies, University of Kent, Canterbury, Kent CT2 7NF, UKDepartment of Biosciences, University of Kent, Canterbury, Kent CT2 7PD, UKSchool of Health, Nursing and Midwifery, University of the West of Scotland, Ayr Campus, Ayr KA8 0SX, Scotland, UKCentre for Health Services Studies, University of Kent, Canterbury, Kent CT2 7NF, UKAn HPLC method was developed and validated for the concurrent detection and quantitation of seven water-soluble vitamins (C, B1, B2, B5, B6, B9, B12) in biological matrices (plasma and urine). Separation was achieved at 30°C on a reversed-phase C18-A column using combined isocratic and linear gradient elution with a mobile phase consisting of 0.01% TFA aqueous and 100% methanol. Total run time was 35 minutes. Detection was performed with diode array set at 280 nm. Each vitamin was quantitatively determined at its maximum wavelength. Spectral comparison was used for peak identification in real samples (24 plasma and urine samples from abstinent alcohol-dependent males). Interday and intraday precision were <4% and <7%, respectively, for all vitamins. Recovery percentages ranged from 93% to 100%.http://dx.doi.org/10.1100/2012/359721 |
| spellingShingle | Margherita Grotzkyj Giorgi Kevin Howland Colin Martin Adrian B. Bonner A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application The Scientific World Journal |
| title | A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application |
| title_full | A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application |
| title_fullStr | A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application |
| title_full_unstemmed | A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application |
| title_short | A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application |
| title_sort | novel hplc method for the concurrent analysis and quantitation of seven water soluble vitamins in biological fluids plasma and urine a validation study and application |
| url | http://dx.doi.org/10.1100/2012/359721 |
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