Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples
In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expec...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-09-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2017-0029 |
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| _version_ | 1832572121558024192 |
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| author | Salmani Jumah Masoud Mohammad Wu Xue Jacob Joe Antony Fu Rong Chen Baoan |
| author_facet | Salmani Jumah Masoud Mohammad Wu Xue Jacob Joe Antony Fu Rong Chen Baoan |
| author_sort | Salmani Jumah Masoud Mohammad |
| collection | DOAJ |
| description | In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experience in vivo while pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL−1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods. |
| format | Article |
| id | doaj-art-2ed0ab38fbf64736900aae513ff8c472 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-2ed0ab38fbf64736900aae513ff8c4722025-02-02T11:50:20ZengSciendoActa Pharmaceutica1846-95582017-09-0167337338410.1515/acph-2017-0029acph-2017-0029Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samplesSalmani Jumah Masoud Mohammad0Wu Xue1Jacob Joe Antony2Fu Rong3Chen Baoan4Department of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School, Southeast University, Nanjing, People’s Republic of ChinaDepartment of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School, Southeast University, Nanjing, People’s Republic of ChinaDepartment of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School, Southeast University, Nanjing, People’s Republic of ChinaState Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention China Pharmaceutical University, Nanjing, People’s Republic of ChinaDepartment of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School, Southeast University, Nanjing, People’s Republic of ChinaIn the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experience in vivo while pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL−1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.https://doi.org/10.1515/acph-2017-0029wogoninhplc/uvstandard sample preparationsample compatibility |
| spellingShingle | Salmani Jumah Masoud Mohammad Wu Xue Jacob Joe Antony Fu Rong Chen Baoan Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples Acta Pharmaceutica wogonin hplc/uv standard sample preparation sample compatibility |
| title | Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples |
| title_full | Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples |
| title_fullStr | Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples |
| title_full_unstemmed | Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples |
| title_short | Development of a new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples |
| title_sort | development of a new hplc method for wogonin in rat plasma compatibility of standard and test samples |
| topic | wogonin hplc/uv standard sample preparation sample compatibility |
| url | https://doi.org/10.1515/acph-2017-0029 |
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